Concurrent Resolution of Chronic Diarrhea Likely Due to Crohn’s Disease and Infection with Mycobacterium avium paratuberculosis

Singh, Shoor Vir; Kuenstner, J. Todd; Davis, William C.; Agarwal, Prabhat; Kumar, Naveen; Singh, Devendra Kumar; Gupta, Saurabh; Chaubey, Kundan Kumar; Kumar, Ashok; Misri, Jyoti; Jayaraman, Sujatha; Sohal, Jagdip Singh; Dhama, Kuldeep

doi:10.3389/fmed.2016.00049

Cited by 28 publications

(15 citation statements)

References 27 publications

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“…The study also showed that one repeat (7g-4ggt) was common to the original isolates obtained by Chiodini et al (cited in [31,32]). This same repeat sequence was also found in the Map isolate obtained from the patient in the case report described here [12], and this isolate is the predominant form found in the environment, livestock, and humans in India. This similarity suggests that screening will reveal limited diversity in the isolates obtained from humans.…”

Section: The Third Stepsupporting

confidence: 78%

“…Other than the massive shedding of Map in his feces, the clinical features of the patient's disease were indistinguishable from the clinical features of patients with CD. Treatment with anti-Map antibiotics cleared the infection with Map and concurrently cured the patient of the clinical features of CD [12]. In two other case reports, patients with classic CD and blood cultures positive for Map were treated with anti-Map therapy.…”

Section: Is It Johne's Disease or Crohn's Disease?mentioning

confidence: 99%

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Resolution of Crohn’s (Johne’s) disease with antibiotics: what are the next steps?

Davis

Kuenstner

Singh

2017

Expert Review of Gastroenterology & Hepatology

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Section: The Third Stepsupporting

confidence: 78%

Section: Is It Johne's Disease or Crohn's Disease?mentioning

confidence: 99%

Resolution of Crohn’s (Johne’s) disease with antibiotics: what are the next steps?

Davis

Kuenstner

Singh

2017

Expert Review of Gastroenterology & Hepatology

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“…A strong association has been documented between MAP and Crohn's disease (Waddell et al, 2015), but important knowledge gaps to establish the causal path still exist (More et al, 2017). Genetic mimicry between protein epitopes of MAP and human proteins have been associated with autoimmune disorders (Davis, 2015;Sechi and Dow, 2015;Singh et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Association of paratuberculosis sero-status with milk production and somatic cell counts across 5 lactations, using multilevel mixed models, in dairy cows

Martins

Oliveira

et al. 2018

Journal of Dairy Science

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The aim of this work was to investigate associations between individual cow Mycobacterium avium ssp. paratuberculosis (MAP) seropositivity, 305-d corrected milk production, and somatic cell count during 5 lactations lifespan in Portuguese dairy herds using multilevel mixed models. We used MAP serum ELISA (Idexx MAP Ac, Idexx Laboratories Inc., Westbrook, ME) results (n = 23,960) from all the 20,221 adult cows present in 329 farms and corresponding 47,586 lactation records from the National Dairy Improvement Association. Cows and farms were classified as positive or negative. Multilevel mixed models were used to investigate the association of cow MAP status with variation in milk production and somatic cell count. Cow MAP status, farm status, and lactation number were considered as independent variables. A quadratic function of lactation number was used to mimic the effect of lactation order on milk production. The models considered 3 levels: measurement occasion (level 1) within cow (level 2) and cow within farm (level 3). Four final models were produced, including all herds and cows, to address the effect of farm status (models 1 and 2) or the effect of cow status (models 3 and 4) on the outcome variables. Our results show that MAP status affects milk production. Losses are detectable from third lactation onward. During the first 5 lactations, positive cows accumulated an average loss of 1,284.8 kg of milk when compared with the negative cows. We also observed that somatic cell counts were higher in positive cows and a positive interaction occurs between cow status and lactation number, suggesting a positive association between MAP infection and increased somatic cell counts. Our results are in line with previous studies, suggesting a possible positive relation between cow milk production and susceptibility to MAP infection.

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“…Similarly other workers have also reported involvement of MAP in clinical symptoms and gross pathology of Crohn's disease (CD) in human beings and Johne's disease in animals [6][7][8]. In India, MAP has also been recovered from chronic patients of CD [9], suspected animal workers [10], human population from peri-urban areas [11], mass screening of human samples [1] and of patients suffering with advance clinical stage of CD [12].…”

Section: Introductionmentioning

confidence: 95%

Molecular Diversity and Homology in Six CFPs Genes in the Novel BioType, 'Indian Bison Type' of Mycobacterium avium subspecies paratuberculosis of Goat Origin vs. other Biotypes

Sv¹,

Chaubey²,

Kumaresan³

et al. 2018

Mycobact Dis

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Objective: Characterization of novel 'Indian Bison Type' bio-type of Mycobacterium avium subspecies paratuberculosis (MAP) strain 'S5' (goat origin) was done through six MAP cultural filtrate proteins (CFPs) genes (MAP 1693c, MAP 2168c, MAP Mod D, MAP 85c, MAP Pep AN and MAP Pep AC) encoding for above six recombinant CFPs. Material and Methods: Six immunogenic MAP CFPs genes (1693c, 2168c, ModD, 85c, Pep AN and Pep AC) were cloned and were confirmed by restriction digestions and sequencing. Evolutionary Analysis of the six genes was done using phylogenetic tree constructions and Sequence Identity plot. Results and Discussion: Sequence analysis of six CFPs genes was studied extensively for their genetic composition, mutations and other variations in their open reading frames. Good percentage of homology has been found among the global biotypes/strains of the MAP along with the MAP strain 'S5' and a novel biotype (Indian Bison Type) of goat origin and other Indian isolates. Aligned sequences of the six genes have been submitted to NCBI. Genebank accessioned by following IDs (MG753462, MG753463, MG753464, MG753465, MG753466 and MG753467). However, certain insertions, deletions within the genes still required to be explored to identify the nature of the disease in Indian scenario with respect to genotypic influences in geographical entity. Mutations were observed in four genes of MAP 'S5' strain, 2168c, Mod D, Pep AN and Pep AC. Phylogenetic tree analysis of MAP 'S5' genes showed 1693c and 2168c genes were taxonomically distant, on contrary Mod D, Pep AN were located taxonomically closer, 85C was present as out group of the first branch and Pep AC slightly away. Conclusion: The study helped to understand molecular diversity and homology of CFPs genes of MAP 'S5' with other strains. Mutations may lead to modulations in the functions of the MAP metabolism thereby influencing the virulence and host response to the disease.

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Concurrent Resolution of Chronic Diarrhea Likely Due to Crohn’s Disease and Infection with Mycobacterium avium paratuberculosis

Cited by 28 publications

References 27 publications

Resolution of Crohn’s (Johne’s) disease with antibiotics: what are the next steps?

Resolution of Crohn’s (Johne’s) disease with antibiotics: what are the next steps?

Association of paratuberculosis sero-status with milk production and somatic cell counts across 5 lactations, using multilevel mixed models, in dairy cows

Molecular Diversity and Homology in Six CFPs Genes in the Novel BioType, 'Indian Bison Type' of Mycobacterium avium subspecies paratuberculosis of Goat Origin vs. other Biotypes

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