Concurrent treatment of raw and aerated swine wastewater using an electrotrophic denitrification system

Prokhorova, Anna; Kainuma, Mami; Hiyane, Rie; Boerner, Susan; Goryanin, Igor

doi:10.1016/j.biortech.2020.124508

Cited by 17 publications

(9 citation statements)

References 44 publications

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“…The selected AEM was tested for the efficiency of removal of organics from raw WW and nitrate from aerated WW using a 2 L, two-chambered biocathode BEDS ( Figure 3 ); the reactors and procedures were described in our previous paper [ 11 ]. Briefly, carbon fiber (CFS-2; SO-EN Co., Ltd., Gunma, Japan) and a carbon brush with stainless steel core 800 K tips per 2.5 cm (Chieh Wang Industry and Trade Co, Hengshui, China) were used for the anode and cathode, respectively.…”

Section: Methodsmentioning

confidence: 99%

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Development of a New Hydrogel Anion Exchange Membrane for Swine Wastewater Treatment

et al. 2022

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We developed a proprietary anion exchange membrane (AEM) for wastewater treatment as an alternative to commercial products. Following the successful development of a hydrogel cation exchange membrane on a porous ceramic support, we used the same approach to fabricate an AEM. Different positively charged monomers and conditions were tested, and all AEMs were tested for nitrate and phosphate anion removal from buffers by electrodialysis. The best AEM was tested further with real swine wastewater for phosphate removal by electrodialysis and nitrate removal in a bioelectrochemical denitrification system (BEDS). Our new AEM showed better phosphate removal compared with a commercial membrane; however, due to its low permselectivity, the migration of cations was detected while operating a two-chambered biocathode BEDS in which the membrane was utilized as a separator. After improving the permselectivity of the membrane, the performance of our proprietary AEM was comparable to that of a commercial membrane. Because of the usage of a porous ceramic support, our AEM is self-supporting, sturdy, and easy to attach to various frames, which makes the membrane better suited for harsh and corrosive environments, such as swine and other animal farms and domestic wastewater.

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Section: Methodsmentioning

confidence: 99%

“…We first tested for phosphate removal and concentration by electrodialysis; concentrated phosphate can be precipitated further as a form of struvite [ 10 ]. The membrane was then tested as a separator in a bioelectrochemical denitrification system (BEDS) developed in our laboratory [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Development of a New Hydrogel Anion Exchange Membrane for Swine Wastewater Treatment

et al. 2022

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“…The dominant species with percentage of total OTUs exceeding 1% were Thauera (29.8%), Methanosaeta (6.4%), and Anaerovorax (1.7%); Halomonas (6.0%); Anaerovorax (18.4), Nitrosomonas (7.0%), and Vulcanibacillus (5.3%); and Paenibacillus (19.0%) and Anaerovorax (12.4%) on storage days 0, 60, 120, and 180, respectively. The most dominant species in the raw SWW, Thauera sp., is a heterotrophic denitrification species [ 24 ], and Thauera sp. is reported to be the functional bacteria in partial denitrification–anammox processes [ 25 , 26 ]; however, the OTUs for this species decrease to very low levels in the stored SWW.…”

Section: Resultsmentioning

confidence: 99%

“…The 16S rRNA gene and the ITS gene were quantified using a StepOnePlus™ RealTime PCR System (Thermo Fisher, Waltham, MA, USA) and TB Green™ Premix Ex Taq™ II (Tli RNaseH Plus) kit (Takara, Kyoto Japan). The qPCR conditions, primer sequences targeting these genes, and PCR protocol were based on previously reported methods [ 24 ]. Each reaction contained 0.8 µL of primers, 1 µL of DNA templates, 5 µL of TB Green Premix Ex Taq II (Takara, Japan), 0.2 µL of ROX Reference Dye (Bio-Rad), and 3 µL of ddH2O (Bio-Rad).…”

Section: Methodsmentioning

confidence: 99%

Anaerobic Storage Completely Removes Suspected Fungal Pathogens but Increases Antibiotic Resistance Gene Levels in Swine Wastewater High in Sulfonamides

Zhao

Zhang

Sun

et al. 2023

IJERPH

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Wastewater storage before reuse is regulated in some countries. Investigations of pathogens and antibiotic resistance genes (ARGs) during wastewater storage are necessary for lowering the risks for wastewater reuse but are still mostly lacking. This study aimed to investigate pathogens, including harmful plant pathogens, and ARGs during 180 d of swine wastewater (SWW) storage in an anaerobic storage experiment. The contents of total organic carbon and total nitrogen in SWW were found to consistently decrease with the extension of storage time. Bacterial abundance and fungal abundance significantly decreased with storage time, which may be mainly attributed to nutrient loss during storage and the long period of exposure to a high level (4653.2 μg/L) of sulfonamides in the SWW, which have an inhibitory effect. It was found that suspected bacterial pathogens (e.g., Escherichia–Shigella spp., Vibrio spp., Arcobacter spp., Clostridium_sensu_stricto_1 spp., and Pseudomonas spp.) and sulfonamide-resistant genes Sul1, Sul2, Sul3, and SulA tended to persist and even become enriched during SWW storage. Interestingly, some suspected plant fungal species (e.g., Fusarium spp., Ustilago spp. and Blumeria spp.) were detected in SWW. Fungi in the SWW, including threatening fungal pathogens, were completely removed after 60 d of anaerobic storage, indicating that storage could lower the risk of using SWW in crop production. The results clearly indicate that storage time is crucial for SWW properties, and long periods of anaerobic storage could lead to substantial nutrient loss and enrichment of bacterial pathogens and ARGs in SWW.

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“…Moreover, it was demonstrated that the anammox process (i.e., anaerobic

oxidation to N 2 ) could be linked to electricity production (Shaw et al, 2020 ). Among the nitrogen pathways, bioelectrochemical denitrification is the most researched, and its suitability for treating, e.g., process water from the nutrition industry (Prokhorova et al, 2021 ), and also its robustness against other contaminants (Ceballos-Escalera et al, 2021 ) were demonstrated.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical and Microbial Dissection of Electrified Biotrickling Filters

Korth

Pous²,

Honig³

et al. 2022

Front. Microbiol.

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Electrified biotrickling filters represent sustainable microbial electrochemical technology for treating organic carbon-deficient ammonium-contaminated waters. However, information on the microbiome of the conductive granule bed cathode remains inexistent. For uncovering this black box and for identifying key process parameters, minimally invasive sampling units were introduced, allowing for the extraction of granules from different reactor layers during reactor operation. Sampled granules were analyzed using cyclic voltammetry and molecular biological tools. Two main redox sites [−288 ± 18 mV and −206 ± 21 mV vs. standard hydrogen electrode (SHE)] related to bioelectrochemical denitrification were identified, exhibiting high activity in a broad pH range (pH 6–10). A genome-centric analysis revealed a complex nitrogen food web and the presence of typical denitrifiers like Pseudomonas nitroreducens and Paracoccus versutus with none of these species being identified as electroactive microorganism so far. These are the first results to provide insights into microbial structure-function relationships within electrified biotrickling filters and underline the robustness and application potential of bioelectrochemical denitrification for environmental remediation.

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Concurrent treatment of raw and aerated swine wastewater using an electrotrophic denitrification system

Cited by 17 publications

References 44 publications

Development of a New Hydrogel Anion Exchange Membrane for Swine Wastewater Treatment

Development of a New Hydrogel Anion Exchange Membrane for Swine Wastewater Treatment

Anaerobic Storage Completely Removes Suspected Fungal Pathogens but Increases Antibiotic Resistance Gene Levels in Swine Wastewater High in Sulfonamides

Electrochemical and Microbial Dissection of Electrified Biotrickling Filters

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