Concurrent treatment with ursolic acid and low-intensity treadmill exercise improves muscle atrophy and related outcomes in rats

Jang

et al. 2020

Sci Rep

Damage to lower limb muscles requires accurate analysis of the muscular condition via objective microscopic diagnosis. However, microscopic tissue analysis may cause deformation of the tissue structure due to injury induced by external factors during tissue sectioning. to substantiate these muscle injuries, we used synchrotron X-ray imaging technology to project extremely small objects, provide three-dimensional microstructural analysis as extracted samples. in this study, we used mice as experimental animals to create soleus muscle models with various nerve injuries. We morphologically analyzed and quantified the damaged Section and Crush muscles, respectively, via three-dimensional visualization using synchrotron radiation X-ray imaging to diagnose muscle injury. Results of this study can also be used as basic data in the medical imaging field.

mentioning

confidence: 99%

Synchrotron radiation imaging analysis of neural damage in mouse soleus muscle

Jang

et al. 2020

Sci Rep

“…UA treatment was carried out as per the same method used in a previous study 11 . UA was dissolved in distilled water containing 0.1% Tween 80 (Sigma, USA) and administered by intraperitoneal injection (5 mg/kg) once per day (Liu et al, 2013).…”

Section: Methodsmentioning

confidence: 99%

“…UA improves insulin/insulin-like growth factor-1 (IGF-1) signaling, reduces atrophy by decreasing the expression of muscle RING-finger protein-1 (MuRF1) and muscle atrophy F-box (MAFbx) 7 , and induces muscle hypertrophy 7 - 10 . The effects of UA treatment when used alone have not been demonstrated in an animal model of atrophy with hindlimb immobilization (IM), but UA in combination with other treatments such as low-intensity treadmill exercise is effective in increasing skeletal muscle mass 11 . If UA shows beneficial effects similar to those exhibited by resistance exercise, it can be proposed as an exercise pill for the prevention of osteoporosis because resistance exercise is one of the best methods to strengthen bones 12 .…”

Section: Introductionmentioning

confidence: 99%

Effects of ursolic acid on muscle mass and bone microstructure in rats with casting-induced muscle atrophy

Kang

Noh

Kim

2019

JENB

Self Cite

[Purpose]Recent studies suggest that ursolic acid (UA) is a potential candidate for a resistance exercise mimetic that can increase muscle mass and alleviate the deleterious effect of skeletal muscle atrophy on bone health. However, these studies evaluated the effects of UA on skeletal muscle and bone tissues, and they have not verified whether such effect could occur concurrently on muscle and bone, as is the case with resistance exercise. Thus, the aim of this study was to analyze the effect of UA injection on muscle mass and bone microstructure using an animal model of atrophy to demonstrate the potential of UA as a resistance exercise mimetic. [Methods]The immobilization (IM) method was used on the left hindlimb of Sprague Dawley (SD) rats for 10 days to induce muscle atrophy, whereas the right hindlimb was used as an internal control (IC). The animal models were divided into two groups, SED (sedentary, n=6) and UA (n=6) to demonstrate the effect of UA on atrophic skeletal muscles. The UA group received a daily intraperitoneal injection of UA (5 mg/kg/day) for 8 weeks. After 10 days of IM, the data collected for the IC were compared with that of IM to determine whether muscle atrophy might occur.[Results]Muscle atrophy was induced and bone mineral density (BMD) decreased significantly. The 8-week UA treatment significantly increased the gastrocnemius muscle mass compared to the SED group. In regard to the effect of UA on bones, negative results such as a decrease in BMD, trabecular bone volume fraction, and trabecular number, and an increase in trabecular separation, were observed in the SED group, but no such difference was observed in the UA group. No significant difference was observed in atrophic hindlimbs between SED and UA groups.[Conclusion]These results alone are insufficient to suggest that UA is a potential resistance exercise mimetic for atrophic skeletal muscle and weakened bone. However, this study will help determine the potential of UA as a resistance exercise mimetic.

“…Another positive action of triterpenoids is the stimulation and maintaining of physical activity. In rodents, treatment with ursolic acid or celastrol improves weight loss, condition of muscle tissue and muscle mass elevation, increases stamina, helps to maintain high motor performance for a longer time [ 260 , 277 , 278 , 279 , 280 ]. In Drosophila , ursolic acid increases in climbing activity.…”

Section: Terpenoids As Potential Geroprotectorsmentioning

confidence: 99%

Terpenoids as Potential Geroprotectors

et al. 2020

Terpenes and terpenoids are the largest groups of plant secondary metabolites. However, unlike polyphenols, they are rarely associated with geroprotective properties. Here we evaluated the conformity of the biological effects of terpenoids with the criteria of geroprotectors, including primary criteria (lifespan-extending effects in model organisms, improvement of aging biomarkers, low toxicity, minimal adverse effects, improvement of the quality of life) and secondary criteria (evolutionarily conserved mechanisms of action, reproducibility of the effects on different models, prevention of age-associated diseases, increasing of stress-resistance). The number of substances that demonstrate the greatest compliance with both primary and secondary criteria of geroprotectors were found among different classes of terpenoids. Thus, terpenoids are an underestimated source of potential geroprotectors that can effectively influence the mechanisms of aging and age-related diseases.