Two Pinot noir clones (AM10/5 and UCD5) were analyzed
for tannin
and methoxypyrazines (MPs) in different grape tissues during berry
development using liquid chromatography and two-dimensional gas chromatography–mass
spectrometry. On a per berry basis, skin tannins reached the maximum
level about 2–3 weeks after véraison, seed tannins at
around véraison, and stem tannins 4 weeks before véraison.
Clone AM10/5 showed significantly higher levels of seed and stem tannins
on a per berry basis at harvest. Tannin concentration and composition
varied among the different tissues. On a per berry basis, stem tannin
levels were comparable to skin tannins but were 3 to 4 times lower
than seed tannins, while stem tannins had an intermediate galloylation
(5–7%) between seed tannins (12–18%) and skin tannins
(2%) and lower prodelphinidin (4–7%) than skin tannins (31–36%).
The mean degree of polymerization of stem tannins was similar to seed
tannins but lower than skin tannins. MPs, including 3-isopropyl-2-methoxypyrazine
(IPMP), 3-s-isobutyl-2-methoxypyrazine (SBMP), and
3-isobutyl-2-methoxypyrazine (IBMP), showed significantly higher concentrations
than their sensory thresholds in grape stems but not in skins. The
MPs development in stems showed an increasing trend toward véraison
and then a decreasing trend toward harvest. Compared to AM 10/5, UCD5
stems showed a higher level of MPs, especially significantly higher
concentrations of IPMP and IBMP at harvest. The extraction of MPs
from grape stems could contribute negative green and vegetative characters
to Pinot noir wines.