Condensed tannin biosynthesis genes are regulated separately from other flavonoid biosynthesis genes in apple fruit skin

Takos, Adam M.; Ubi, Benjamin Ewa; Robinson, Simon P.; Walker, Amanda R.

doi:10.1016/j.plantsci.2005.10.001

Cited by 99 publications

(90 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…5B). Skin sector samples were taken at 48 and 147 d after full bloom (DAFB), which we found in a previous study coincided with two distinct phases of anthocyanin synthesis in Cripps' Red fruit (Takos et al, 2006b). At 48 DAFB MdMYB1 transcript levels were approximately 24-fold higher in red skin than green skin sectors and at 147 DAFB were approximately 4.5-fold higher.…”

Section: Mdmyb1 Expression Analysismentioning

confidence: 69%

“…In apple, there could be orthologs of these MYB regulators that can stimulate transcription from promoters of some of the early flavonoid pathway genes so as to generate the precursor compounds required for flavonol and CT synthesis. These regulators would be expressed early in fruit development when these compounds are made, and in particular transcripts of the genes required for flavonol and CT synthesis are abundant (Takos et al, 2006b). …”

Section: Activity Of the Flavonoid Pathway In Apple Cultivarsmentioning

confidence: 99%

“…However, both Lister et al (1996) and Li et al (2002) found that MdCHI enzyme activity was much lower in non-red than red skin cultivars at fruit ripening. We analyzed one isoform of MdCHI but Southern analysis has indicated that there are at least two sequences similar to MdCHI in the apple genome (Takos et al, 2006b). It is possible that another MdCHI gene isoform we have not analyzed is involved in anthocyanin synthesis and so analysis of other putative MdCHI genes is needed to clarify whether this step is coregulated.…”

Section: Activity Of the Flavonoid Pathway In Apple Cultivarsmentioning

confidence: 99%

“…This is because CT synthesis and MdLAR and MdANR transcripts are distributed almost equally between red and green skin sectors of the same fruit (Takos et al, 2006b) and so would probably require a regulator that is expressed in both these tissue types. Furthermore, transcripts of MdMYB1 were more abundant in the red skin compared to the non-red skin apple cultivars (Fig.…”

Section: Characterization Of Mdmyb1mentioning

confidence: 99%

“…cv Cripps' Red) were collected at the Primary Industries and Resources South Australia Site at Lenswood in the Adelaide Hills, South Australia from November, 2002 until April, 2003 as described by Takos et al (2006b). Fruit bagging experiments were conducted on cultivars Cripps' Red in April, 2003 andCripps' Pink in April, 2004 at the Lenswood site as described by Takos et al (2006a).…”

Section: Apple Fruit Samplesmentioning

confidence: 99%

See 4 more Smart Citations

Light-Induced Expression of a MYB Gene Regulates Anthocyanin Biosynthesis in Red Apples

Takos¹,

Jaffé²,

Jacob³

et al. 2006

Plant Physiol.

908

776

View full text Add to dashboard Cite

Anthocyanins are secondary metabolites found in higher plants that contribute to the colors of flowers and fruits. In apples (Malus domestica Borkh.), several steps of the anthocyanin pathway are coordinately regulated, suggesting control by common transcription factors. A gene encoding an R2R3 MYB transcription factor was isolated from apple (cv Cripps' Pink) and designated MdMYB1. Analysis of the deduced amino acid sequence suggests that this gene encodes an ortholog of anthocyanin regulators in other plants. The expression of MdMYB1 in both Arabidopsis (Arabidopsis thaliana) plants and cultured grape cells induced the ectopic synthesis of anthocyanin. In the grape (Vitis vinifera) cells MdMYB1 stimulated transcription from the promoters of two apple genes encoding anthocyanin biosynthetic enzymes. In ripening apple fruit the transcription of MdMYB1 was correlated with anthocyanin synthesis in red skin sectors of fruit. When dark-grown fruit were exposed to sunlight, MdMYB1 transcript levels increased over several days, correlating with anthocyanin synthesis in the skin. MdMYB1 gene transcripts were more abundant in red skin apple cultivars compared to non-red skin cultivars. Several polymorphisms were identified in the promoter of MdMYB1. A derived cleaved amplified polymorphic sequence marker designed to one of these polymorphisms segregated with the inheritance of skin color in progeny from a cross of an unnamed red skin selection (a sibling of Cripps' Pink) and the non-red skin cultivar Golden Delicious. We conclude that MdMYB1 coordinately regulates genes in the anthocyanin pathway and the expression level of this regulator is the genetic basis for apple skin color.

show abstract

Section: Mdmyb1 Expression Analysismentioning

confidence: 69%

Section: Activity Of the Flavonoid Pathway In Apple Cultivarsmentioning

confidence: 99%