Condensin II plays an essential role in reversible assembly of mitotic chromosomes in situ

Ono, Takao; Sakamoto, Chiyomi; Nakao, Mitsuyoshi; Saitoh, Noriko; Hirano, Takeshi

doi:10.1091/mbc.e17-04-0252

Cited by 31 publications

(30 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The condensin I complex plays an important role in chromosome condensation and segregation. 12,13 The aberrant expression of the subunits of the complex causes incomplete chromosome condensation. 14 NCAPH is a part of the condensin complex.…”

Section: Discussionmentioning

confidence: 99%

Non‐SMC condensin I complex subunit H enhances proliferation, migration, and invasion of hepatocellular carcinoma

Sun

Huang

Wang

et al. 2019

Molecular Carcinogenesis

View full text Add to dashboard Cite

Non‐SMC condensing I complex subunit H (NCAPH) is a member of the Barr protein family and part of the condensin I complex. The upregulation of NCAPH is associated with poor prognosis in patients with colon cancer. However, the relationship between NCAPH and hepatocellular carcinoma (HCC) remains unclear. This study aimed to explore NCAPH expression in HCC tissues and to investigate NCAPH functions in HCC cells. In this study, we found that high expression of NCAPH in HCC indicated worse prognosis via bioinformatics analysis. Consistently, quantitative real‐time polymerase chain reaction assays in 20 pairs of HCC specimens and the immunohistochemical analysis of 100 HCC tissues showed the upregulation of NCAPH. We established stable NCAPH‐overexpressing and NCAPH knockdown cell lines. Cell Counting Kit‐8 assays and colony formation assay were performed to analyze cell proliferation. Migration and invasion were analyzed by Transwell assays. Subcutaneous xenograft models were used to explore the role of NCAPH in tumor formation in vivo. Our results showed that NCAPH promoted tumor proliferation, migration, and invasion in vitro and in vivo. In conclusion, our findings indicate that NCAPH could serve as a novel prognostic biomarker and a potential therapeutic target for patients with HCC.

show abstract

Section: Discussionmentioning

confidence: 99%

Non‐SMC condensin I complex subunit H enhances proliferation, migration, and invasion of hepatocellular carcinoma

Sun

Huang

Wang

et al. 2019

Molecular Carcinogenesis

View full text Add to dashboard Cite

show abstract

“…Depletion of condensins has been show to result in “fuzzy” chromosomes in organisms from Drosophila to human (Somma et al, 2003, Hudson et al, 2003, Ono et al, 2003), with recent experiments showing that metaphase chromosome structure is particularly sensitive to depletion of condensin II (Ono et al, 2017). It has been established that condensin I and II are differentially distributed along mitotic chromatids (Ono et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…These conclusions were mainly reached from imaging studies of fixed cells or using chromosome reconstitution in cell extracts. It is still unclear how condensins globally organize mitotic chromosome structure and quantitatively contribute to chromosome mechanical stiffness and stability, although a few studies have shown that metaphase chromosomes depleted in condensin have a more swollen conformation and are more easily deformed by hydrodynamic shear flow than unperturbed chromosomes (Green et al, 2012, Hudson et al, 2003, Ono et al, 2017). …”

Section: Introductionmentioning

confidence: 99%

Condensin controls mitotic chromosome stiffness and stability without forming a structurally contiguous scaffold

et al. 2018

View full text Add to dashboard Cite

During cell division, chromosomes must be folded into their compact mitotic form to ensure their segregation. This process is thought to be largely controlled by the action of condensin SMC protein complexes on chromatin fibers. However, how condensins organize metaphase chromosomes is not understood. We have combined micromanipulation of single human mitotic chromosomes, sub-nanonewton force measurement, siRNA interference of condensin subunit expression, and fluorescence microscopy, to analyze the role of condensin in large-scale chromosome organization. Condensin depletion leads to a dramatic (~ 10-fold) reduction in chromosome elastic stiffness relative to the native, non-depleted case. We also find that prolonged metaphase stalling of cells leads to overloading of chromosomes with condensin, with abnormally high chromosome stiffness. These results demonstrate that condensin is a main element controlling the stiffness of mitotic chromosomes. Isolated, slightly stretched chromosomes display a discontinuous condensing staining pattern, suggesting that condensins organize mitotic chromosomes by forming isolated compaction centers that do not form a continuous scaffold.

show abstract

“…31 We have also measured morphological changes of the nucleolus and mitotic chromosomes upon depletion of the cellular components in cell lines. 32,33 Here using wndchrm, we quantitatively investigated morphological features and classification of gastric cancer tissues that included heterogeneous cell populations. Our results indicated that wndchrm reliably computes morphological changes of tumors with differentiation grades, and that cancer-associated protein-based analysis emphasized a correlation between molecular expression and tissue structures.…”

mentioning

confidence: 99%

Computational analysis of morphological and molecular features in gastric cancer tissues

et al. 2020

Self Cite

View full text Add to dashboard Cite

Biological morphologies of cells and tissues represent their physiological and pathological conditions. The importance of quantitative assessment of morphological information has been highly recognized in clinical diagnosis and therapeutic strategies. In this study, we used a supervised machine learning algorithm wndchrm to classify hematoxylin and eosin (H&E)‐stained images of human gastric cancer tissues. This analysis distinguished between noncancer and cancer tissues with different histological grades. We then classified the H&E‐stained images by expression levels of cancer‐associated nuclear ATF7IP/MCAF1 and membranous PD‐L1 proteins using immunohistochemistry of serial sections. Interestingly, classes with low and high expressions of each protein exhibited significant morphological dissimilarity in H&E images. These results indicated that morphological features in cancer tissues are correlated with expression of specific cancer‐associated proteins, suggesting the usefulness of biomolecular‐based morphological classification.

show abstract

Condensin II plays an essential role in reversible assembly of mitotic chromosomes in situ

Cited by 31 publications

References 53 publications

Non‐SMC condensin I complex subunit H enhances proliferation, migration, and invasion of hepatocellular carcinoma

Non‐SMC condensin I complex subunit H enhances proliferation, migration, and invasion of hepatocellular carcinoma

Condensin controls mitotic chromosome stiffness and stability without forming a structurally contiguous scaffold

Computational analysis of morphological and molecular features in gastric cancer tissues

Contact Info

Product

Resources

About