The objective of this present study is to point out some biochemical features of three reared trouts (brook trout, rainbow trout, and Black Sea trout) which are economically important and reared fish species for Eastern Black Sea region. Rearing the rainbow trout has a long history with a great success but brook trout and Black Sea trout are relatively new fish species with an exciting hope for their possible potential in the region. In order to assess the differences among the fish, proximate composition (protein, lipid, moisture, and ash), carbohydrate, energy, carotenes (Vitamin A), fatty acid levels, and lipid indices [atherogenic index (AI), thrombogenic index (TI) and polyene index (PI)] were determined. Additionally, the color of the fillets was also measured in this study. The average levels of moisture and protein differed while those of lipid and ash were close to each other. The highest levels of carbohydrate, energy, and carotenes were determined in muscle of brook trout in this study. The highest meat yield was also obtained from brook trout followed by Black Sea and rainbow trout. A total of 19 fatty acids were determined for Black Sea trout and brook trout and 17 fatty acids for rainbow trout in the study. The omega-3 levels of the all trout used for the study were roughly twice as many as that of the omega-6, except rainbow trout.