2001
DOI: 10.1038/35093537
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Conditional control of gene expression in the mouse

Abstract: One of the most powerful tools that the molecular biology revolution has given us is the ability to turn genes on and off at our discretion. In the mouse, this has been accomplished by using binary systems in which gene expression is dependent on the interaction of two components, resulting in either transcriptional transactivation or DNA recombination. During recent years, these systems have been used to analyse complex and multi-staged biological processes, such as embryogenesis and cancer, with unprecedente… Show more

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Cited by 716 publications
(487 citation statements)
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“…SLICK is based on the popular cre/loxP system that has been used extensively to achieve conditional gene knockout in mice 17,34,35 . The availability of mice harboring conditional knockout alleles is continually increasing and a large scale project to generate "floxed" conditional knockout alleles is underway 1 .…”
Section: Discussionmentioning
confidence: 99%
“…SLICK is based on the popular cre/loxP system that has been used extensively to achieve conditional gene knockout in mice 17,34,35 . The availability of mice harboring conditional knockout alleles is continually increasing and a large scale project to generate "floxed" conditional knockout alleles is underway 1 .…”
Section: Discussionmentioning
confidence: 99%
“…More recently, conditional gene targeting using the Cre/loxP system has emerged as an especially powerful method in reproductive genetics, particularly in the study of genes whose systemic inactivation is lethal. Critical to the success this approach is the availability of suitable Cre transgenes expressed in a wide range of distinct cell lineages at appropriate developmental timepoints (Kwan, 2002;Lewandoski, 2001). Previous germ cell Cre lines include ZP3-Cre, which is not active in primordial follicles but becomes induced following follicle growth; this line is thus useful for analyses of follicle growth or maternal contributions to the embryo (de Vries et al, 2000).…”
mentioning
confidence: 99%
“…These data demonstrate an important role for C/EBPb in keratinocytes survival and skin tumorigenesis. However, phenotypes of germline knockouts can be complicated by pleiotropic effects of the protein under study in various tissues and organs (Lewandoski, 2001). For example, germline deletion of Rb results in severe defects in neurogenesis involving increased apoptosis.…”
mentioning
confidence: 99%