Conditional filamentation as an adaptive trait of bacteria and its ecological significance in soils

Karasz, David C.; Weaver, Anna; Buckley, Daniel H.; Wilhelm, Roland C.

doi:10.1111/1462-2920.16228

Cited by 2 publications

(3 citation statements)

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“…3 Filamentation is also prevalent in soil bacteria to promote mutualistic interactions between bacteria and nutrient uptake in heterogeneous soil environments. 5 Sorting of these elongated cells from the rest of the population will facilitate the study of the mechanisms behind these phenotypes and give more insight into population heterogeneity. Notably, coupling cell sorting from infectious samples with phenotypic characterization and RNA-sequencing techniques can give interesting insights into the virulence of important pathogens.…”

Section: Discussionmentioning

confidence: 99%

“…1 One such phenotype is cell filamentation, a naturally occurring phenomenon in response to stress but also a fitness strategy to prevent phagocytosis by predators, optimize colonization of a niche, and facilitate symbiotic interactions. [2][3][4][5] Size and shape-based sorting can therefore be applied in the study of filamentation in contexts where only a subpopulation of microorganisms is filamentous.…”

Section: Introductionmentioning

confidence: 99%

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Shear-enhanced sorting of ovoid and filamentous bacterial cells using pinch flow fractionation

Timary¹,

Rousseau²,

Melderen³

et al. 2023

Lab Chip

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Shear-enhanced sorting of ovoid and filamentous bacterial cells using pinch flow fractionation

Timary¹,

Rousseau²,

Melderen³

et al. 2023

Lab Chip

View full text Add to dashboard Cite

show abstract

“…B). Drug-induced filamentation can be triggered by multiple molecular mechanisms 55 , including a two-component signal transduction system 56 that blocks FtsZ polymerization and prevents septation in the presence of AMP. After the stress is removed, filamented cells rearrange the FtsZ ring, divide, and resume normal growth 57,58 .…”

Section: Plasmid-driven Phenotypic Noise Produces a Heterogeneous Str...mentioning

confidence: 99%

Plasmid-mediated phenotypic noise leads to transient antibiotic resistance in bacteria

Hernandez-Beltran,

Rodríguez-Beltrán,

Aguilar-Luviano

et al. 2024

Nat Commun

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The rise of antibiotic resistance is a critical public health concern, requiring an understanding of mechanisms that enable bacteria to tolerate antimicrobial agents. Bacteria use diverse strategies, including the amplification of drug-resistance genes. In this paper, we showed that multicopy plasmids, often carrying antibiotic resistance genes in clinical bacteria, can rapidly amplify genes, leading to plasmid-mediated phenotypic noise and transient antibiotic resistance. By combining stochastic simulations of a computational model with high-throughput single-cell measurements of blaTEM-1 expression in Escherichia coli MG1655, we showed that plasmid copy number variability stably maintains populations composed of cells with both low and high plasmid copy numbers. This diversity in plasmid copy number enhances the probability of bacterial survival in the presence of antibiotics, while also rapidly reducing the burden of carrying multiple plasmids in drug-free environments. Our results further support the tenet that multicopy plasmids not only act as vehicles for the horizontal transfer of genetic information between cells but also as drivers of bacterial adaptation, enabling rapid modulation of gene copy numbers. Understanding the role of multicopy plasmids in antibiotic resistance is critical, and our study provides insights into how bacteria can transiently survive lethal concentrations of antibiotics.

show abstract

Conditional filamentation as an adaptive trait of bacteria and its ecological significance in soils

Cited by 2 publications

References 1 publication

Shear-enhanced sorting of ovoid and filamentous bacterial cells using pinch flow fractionation

Shear-enhanced sorting of ovoid and filamentous bacterial cells using pinch flow fractionation

Plasmid-mediated phenotypic noise leads to transient antibiotic resistance in bacteria

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