Conditioned media from mesenchymal stromal cells and periodontal ligament fibroblasts under cyclic stretch stimulation promote bone healing in mouse calvarial defects

Ogisu, Kota; Fujio, Masahito; Tsuchiya, Shunji; Tsuboi, Masahiro; Chang, Qi; Toyama, Naoto; Kamio, Hisanobu; Hibi, Hideharu

doi:10.1016/j.jcyt.2020.05.008

Cited by 16 publications

(11 citation statements)

References 28 publications

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“…CM in a collagen sponge was administered to a mouse calvarial defect model, and the results showed that bone regeneration and angiogenesis were enhanced by CM obtained from the cyclic stretch culture group. Indeed, CM obtained from cells cultured in the cyclic stretch stimulation contained more BMP-2, BMP-4, and VEGF-A [ 114 ].…”

Section: Future Perspective: Cell-free Approachmentioning

confidence: 99%

Oral Bone Tissue Regeneration: Mesenchymal Stem Cells, Secretome, and Biomaterials

Gugliandolo

Fonticoli

Trubiani

et al. 2021

IJMS

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In the last few decades, tissue engineering has become one of the most studied medical fields. Even if bone shows self-remodeling properties, in some cases, due to injuries or anomalies, bone regeneration can be required. In particular, oral bone regeneration is needed in the dentistry field, where the functional restoration of tissues near the tooth represents a limit for many dental implants. In this context, the application of biomaterials and mesenchymal stem cells (MSCs) appears promising for bone regeneration. This review focused on in vivo studies that evaluated bone regeneration using biomaterials with MSCs. Different biocompatible biomaterials were enriched with MSCs from different sources. These constructs showed an enhanced bone regenerative power in in vivo models. However, we discussed also a future perspective in tissue engineering using the MSC secretome, namely the conditioned medium and extracellular vesicles. This new approach has already shown promising results for bone tissue regeneration in experimental models.

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Section: Future Perspective: Cell-free Approachmentioning

confidence: 99%

Oral Bone Tissue Regeneration: Mesenchymal Stem Cells, Secretome, and Biomaterials

Gugliandolo

Fonticoli

Trubiani

et al. 2021

IJMS

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“…This approach was examined using a rodent preclinical calvarial defect model [170]. Alternatively conditioned media derived from hMSC under cyclic stretch conditions may also be advantageous in stimulating cranial bone repair and vascular infiltration through the secretion of paracrine factors that promote osteogenesis and angiogenesis [171]. The use of endogenous and exogenous MSC have also been utilized to treat critical sized defects within the cranium with some success.…”

Section: Repair Of Craniofacial Bonementioning

confidence: 99%

Clinical Application of Bone Marrow Mesenchymal Stem/Stromal Cells to Repair Skeletal Tissue

Arthur

Gronthos

2020

IJMS

173

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There has been an escalation in reports over the last decade examining the efficacy of bone marrow derived mesenchymal stem/stromal cells (BMSC) in bone tissue engineering and regenerative medicine-based applications. The multipotent differentiation potential, myelosupportive capacity, anti-inflammatory and immune-modulatory properties of BMSC underpins their versatile nature as therapeutic agents. This review addresses the current limitations and challenges of exogenous autologous and allogeneic BMSC based regenerative skeletal therapies in combination with bioactive molecules, cellular derivatives, genetic manipulation, biocompatible hydrogels, solid and composite scaffolds. The review highlights the current approaches and recent developments in utilizing endogenous BMSC activation or exogenous BMSC for the repair of long bone and vertebrae fractures due to osteoporosis or trauma. Current advances employing BMSC based therapies for bone regeneration of craniofacial defects is also discussed. Moreover, this review discusses the latest developments utilizing BMSC therapies in the preclinical and clinical settings, including the treatment of bone related diseases such as Osteogenesis Imperfecta.

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“…In a dog model of bilateral maxillary sinus floor augmentation, Yu et al showed that seeding of PDLSCs or BMMSCs onto Bio-Oss can promote bone formation and mineralization and maintain the maximum volume of the augmented maxillary sinus (112). Meanwhile, several studies have shown that transplantation of PDLSC CM or various PDLSC-seeded scaffolds can significantly enhance bone repair/regeneration in calvarial defects in mice (113,114), rat (115,116), and rabbit (117). These compelling findings have demonstrated that PDLSCs have potentials in the regeneration/reconstruction of oral maxillofacial tissues, particularly, lost/damaged support tissue in the periodontium complex, including the alveolar bone, periodontal ligament, and cementum (119).…”

Section: Periodontal Ligament Stem Cells (Pdlsc)mentioning

confidence: 99%

Potential application of dental stem cells in regenerative reconstruction of oral and maxillofacial tissues: a narrative review

He¹,

Zhang²,

Motiwala³

et al. 2022

Front Oral Maxillofac Med

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Oral and maxillofacial (OMF) defects caused by congenital conditions and various injuries such as trauma, warfare, ablative surgery for benign and malignant head & neck tumor can often lead to OMF deformities and malfunctions in speech, mastication/chewing, and swallowing as well as deleterious psychological effects and socioeconomic burdens to patients. Due to the unique complex 3D geometry and complicated causes, reconstruction and rehabilitation of OMF defects remain a major challenge for OMF surgeons. Currently, microsurgical free tissue transfer remains the mainstay of care in the reconstruction of OMF defects due to their abundant blood supply and flexibility for transplantation. However, there exists several major challenges, such as the limited availability, the requirement of a secondary surgery and donor site morbidity, and the multifactorial failure of free flap transplantation. Due to the advancement in stem cell biology, biomaterial science, and tissue engineering (TE) technology, stem cell-based regenerative therapy is emerging as a promising therapeutic approach for a variety of diseases, including regenerative reconstruction and rehabilitation of OMF defects. In this narrative review, we update the information on the characteristics and biological functions of mesenchymal stem cells derived from various dental tissues (dental-MSCs) and their released cell-free products, extracellular vesicles (EVs). We then highlight their potential application in TE and regenerative reconstruction of OMF defects in animal models and clinical studies and the potential challenges in this field.

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Conditioned media from mesenchymal stromal cells and periodontal ligament fibroblasts under cyclic stretch stimulation promote bone healing in mouse calvarial defects

Cited by 16 publications

References 28 publications

Oral Bone Tissue Regeneration: Mesenchymal Stem Cells, Secretome, and Biomaterials

Oral Bone Tissue Regeneration: Mesenchymal Stem Cells, Secretome, and Biomaterials

Clinical Application of Bone Marrow Mesenchymal Stem/Stromal Cells to Repair Skeletal Tissue

Potential application of dental stem cells in regenerative reconstruction of oral and maxillofacial tissues: a narrative review

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