Conditioned medium from the human umbilical cord-mesenchymal stem cells stimulate the proliferation of human keratinocytes

Abstract

<jats:title>Abstract</jats:title><jats:sec id="j_jbcpp-2019-0283_abs_001_w2aab3b7b7b1b6b1aab1c12b2Aa"><jats:title>Objectives</jats:title><jats:p>Wound healing is a complex process with a sequence of restoring and inhibition events such as cell proliferation, differentiation, migration as well as adhesion. Mesenchymal stem cells (MSC) derived conditioned medium (CM) has potent therapeutic functions and promotes cell proliferation, anti-oxidant, immunosuppressive, and anti-apoptotic effects. The main aim of this research is to study the role of human umbilical cord-mesenchymal stem cells (UC-MSCs) derived CM in stimulating the proliferation of human keratinocytes (HaCaT).</jats:p></jats:sec><jats:sec id="j_jbcpp-2019-0283_abs_002_w2aab3b7b7b1b6b1aab1c12b3Aa"><jats:title>Methods</jats:title><jats:p>Firstly, MSC were isolated from human umbilical cords (UC) and the cells were then cultured in proliferative medium. We prepared and collected the CM after 72 h. Morphological changes were observed after the treatment of HaCaT cells with CM. To validate the findings, proliferation rate, clonal efficiency and also gene expression studies were performed.</jats:p></jats:sec><jats:sec id="j_jbcpp-2019-0283_abs_003_w2aab3b7b7b1b6b1aab1c12b4Aa"><jats:title>Results</jats:title><jats:p>Increased proliferation rate was observed and confirmed with the expression of Proliferating Cell Nuclear Antigen (<jats:italic>PCNA</jats:italic>) after treatment with HaCaT cells. Cell-cell strap formation was also observed when HaCaT cells were treated with CM for a period of 5–6 days which was confirmed by the increased expression of Collagen Type 1 Alpha 1 chain (<jats:italic>Col1A1</jats:italic>).</jats:p></jats:sec><jats:sec id="j_jbcpp-2019-0283_abs_004_w2aab3b7b7b1b6b1aab1c12b5Aa"><jats:title>Conclusions</jats:title><jats:p>Our results from present study depicts that the secretory components in the CM might play a significant role by interacting with keratinocytes to promote proliferation and migration. Thus, the CM stimulates cellular proliferation, epithelialization and migration of skin cells which might be the future promising application in wound healing.</jats:p></jats:sec>

Umbilical cord‐derived mesenchymal stem cell secretome promotes skin regeneration and rejuvenation: From mechanism to therapeutics

“…26,27 Because the UCMSC secretome has been found to enhance cutaneous wound healing, it is essential to understand the wound healing process. 23,28 Wound healing is an intricate, dynamic, and harmonious process that involves 4 distinct and overlapping stages: haemostasis, inflammation, proliferation, and remodelling. During the haemostasis stage, damaged tissue and cells release vasoactive chemicals that promote local vasoconstriction, while platelets coagulate, activating the blood coagulation system and forming clots that stop bleeding and protect the wounded area.…”

“…Keratinocytes, the predominant component of the skin epithelium, have significance for re‐epithelialization. The UCMSC secretome mainly promotes the proliferation and migration of keratinocytes 28,47,66 . Keratinocytes will form cell–cell straps when they migrate to the wound surface 80 .…”

“…The cell–cell strap is a collagen strap formed by the cell traction force between keratinocytes. For assistance in cell migration, the cells pull the collagen fibres into a sort of bundle around themselves, which UCMSC‐CM can facilitate 28,81 . Furthermore, UCMSC‐CM has an even greater capacity to promote keratinocyte migration than fibroblasts.…”

“…Acute wound is often caused by physical or chemical damage, as well as surgical treatments, whereas chronic wound is typically caused by diseases such as infections, diabetes, and vascular disease 26,27 . Because the UCMSC secretome has been found to enhance cutaneous wound healing, it is essential to understand the wound healing process 23,28 …”

In vitro characterization of umilical cord-dervied MSC's supplemented with PLAY®:A potential FBS substitute

“…The source of MSCs dictates their various properties. Umbilical cord-derived MSCs (UC-MSCs) are considered to have a more potent proliferative, immunomodulatory, and self-renewal capacity compared to MSCs derived from other tissues (20,21). Furthermore, recent evidence has demonstrated the safety of UC-MSCs in clinical usage and immunocompromised patients (22,23).…”