Conditioned medium of E17 rat brain cells induced differentiation of primary colony of mice blastocyst into neuron-like cells

Abstract

Background Conditioned medium is the medium obtained from certain cultured cells and contained secretome from the cells. The secretome, which can be in the form of growth factors, cytokines, exosomes, or other proteins secreted by the cells, can induce the differentiation of cells that still have pluripotent or multipotent properties. Objectives This study examined the effects of conditioned medium derived from E17 rat brain cells on cells with pluripotent properties. Methods The conditioned medium used in this study originated from E17 rat brain cells. The CM was used to induce the differentiation of primary colonies of mice blastocysts. Primary colonies were stained with alkaline phosphatase to analyze the pluripotency. The morphological changes in the colonies were examined, and the colonies were stained with GFAP and Neu-N markers on days two and seven after adding the conditioned medium. Results The conditioned medium could differentiate the primary colony, beginning with the formation of embryoid-body-like structure; round GFAP positive cells were identified. Finally, neuron-like cells testing positive for Neu-N were observed on the seventh day after adding the conditioned medium. Conclusions Conditioned medium from different species, in this case, E17 rat brain cells, induced and promoted the differentiation of the primary colony from mice blastocysts into neuron-like cells. The addition of CM mediated neurite growth in the differentiation process.

Immunocytochemistry Studies Using Microtubule-Associated Protein-2 (MAP-2) Markers on Neural Differentiation of Mesenchymal Stem Cells from Rat Bone Marrow

“…Rinendyaputri et al (2018) reported that growth factors including bFGF and NGF were present in conditioned medium-mesenchymal stem cells (CM-MSCs), which can prevent neuronal cells from degenerating and promote the differentiation of neural stem/progenitor cells (NPCs) into astrocytes and neurons [16]. CM from neural progenitor cells (NPC) is also reported can stimulate differentiation to astrocytes and neurons and can maintain cell growth [17,18]. showed that the addition of bFGF, EGF, and BDNF to the medium induced the differentiation of rat bone marrow MSCs into neurons and glia and characterized neural markers like A2B5, beta-III-tubulin, PSA-NCAM, and MAP-2) [19].…”