Conditioned Medium of Human Amniotic Epithelial Cells Alleviates Experimental Allergic Conjunctivitis Mainly by IL-1ra and IL-10

Wu, Binxin; Gao, Furong; Lin, Jianhua; Lü, Lixia; Xu, Huiming; Xu, Guo‐Tong

doi:10.3389/fimmu.2021.774601

Cited by 10 publications

(6 citation statements)

References 66 publications

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“…Additional characteristics are its ability to inhibit IgE release by effector B cells and mast cell activation and function, as well as exhibit an antihistamine effect. As such, data thus far supports continued research in this area as a potential, novel treatment for AC [ 203 ].…”

Section: Potential Therapeutic Targetsmentioning

confidence: 76%

“…Wu et al hypothesized that there is a potential benefit from tissue stem cell conditioned medium (CM) on AC. In a study comparing various types of topically applied tissue stem cell CM, it was the human amniotic epithelial cell CM that demonstrated a significant reduction in the signs and symptoms of AC [ 203 ]. The utility in using tissue stem cells in general is their ability to self-renew and differentiate, thus allowing the repair of various types of damage.…”

Section: Potential Therapeutic Targetsmentioning

confidence: 99%

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Therapeutic Targets in Allergic Conjunctivitis

Labib

Chigbu

2022

Pharmaceuticals

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Allergic conjunctivitis (AC) is a common condition resulting from exposure to allergens such as pollen, animal dander, or mold. It is typically mediated by allergen-induced crosslinking of immunoglobulin E attached to receptors on primed conjunctival mast cells, which results in mast cell degranulation and histamine release, as well as the release of lipid mediators, cytokines, and chemokines. The clinical result is conjunctival hyperemia, tearing, intense itching, and chemosis. Refractory and chronic cases can result in ocular surface complications that may be vision threatening. Patients who experience even mild forms of this disease report an impact on their quality of life. Current treatment options range from non-pharmacologic therapies to ocular and systemic options. However, to adequately control AC, the use of multiple agents is often required. As such, a precise understanding of the immune mechanisms responsible for this ocular surface inflammation is needed to support ongoing research for potential therapeutic targets such as chemokine receptors, cytokine receptors, non-receptor tyrosine kinases, and integrins. This review utilized several published articles regarding the current therapeutic options to treat AC, as well as the pathological and immune mechanisms relevant to AC. This review will also focus on cellular and molecular targets in AC, with particular emphasis on potential therapeutic agents that can attenuate the pathology and immune mechanisms driven by cells, receptors, and molecules that participate in the immunopathogenesis and immunopathology of AC.

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Section: Potential Therapeutic Targetsmentioning

confidence: 76%

Section: Potential Therapeutic Targetsmentioning

confidence: 99%

Therapeutic Targets in Allergic Conjunctivitis

Labib

Chigbu

2022

Pharmaceuticals

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“…Notably, treatment with CM secreted by AECs increased the levels of anti-inflammatory factors TGF-β and IL-10 as well as the expression of Foxp3, a protein involved in immune system responses. 28 A study showed that soluble factors contained in AM-CM significantly reduced the inflammatory response on human limbal myofibroblast by inhibiting NF-κB activity and subsequently abrogating the synthesis and secretion of proinflammatory chemokines CCL2, CCL5, and CXCL10. 29 Another report has shown that the addition of rat AECs-CM to lipopolysaccharide-activated macrophages evoked anti-inflammatory effects via a decrease in TNF-α expression and inhibited tumor cell proliferation in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

Antiproliferative and apoptotic effects of conditioned medium released from human amniotic epithelial stem cells on breast and cervical cancer cells

Jafari

Niknejad

Rezaei‐Tavirani

et al. 2023

Int J Immunopathol Pharmacol

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Introduction Human amniotic membrane (hAM) and its cells have been proposed for several clinical applications, including cancer therapy. However, reports on the anticancer effects of human amniotic epithelial stem cells-conditioned media (hAECs-CM) are limited. This work aims to evaluate the anticancer effects of hAECs-CM on cervical cancer and breast cancer cell lines in vitro . Methods Human term placentas were gained from uncomplicated Cesarean sections from healthy donor women. After amnion peeling from the chorion, its epithelial stem cells were isolated and cultured, and its conditioned medium (CM) was collected for experiments. MTT assay was performed to assess cancer cells viability. Migration rate of cancer cells was examined via wound healing assay. Cell-cycle distribution and apoptosis were determined using flow cytometry. Results Based on MTT assay hAECs-CM was cytotoxic against cancerous cell lines in a dose-time-dependent manner. After 48 h of treatment with hAECs-CM pure, the cell viability of breast cancer cells includes MCF-7 and MDA-MB-231 reached to 73.2% and 65.5%, respectively. In the same situation, HeLa cervical cancer cell line revealed the lowest viability by 47.3%. The wound-healing assay displayed an incomplete wound closure of scratched MDA-MB-231 cells and significant inhibition of cell migration after hAECs-CM treatment. The results also revealed that hAECs-CM exerted anti-proliferation activity by prompting cell cycle arrest and apoptosis of cancer cells. Conclusions: hAECs-CM is a potent candidate for inducing apoptosis and simultaneously inhibition of the proliferation and migration of cancer cells via inhibiting cell cycle blockade.

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“…In some preclinical studies, Oksana et al found that the secretome derived from MSCs reduces disease severity in inflammatory arthritis mice, enhances Treg function, and restores the ratio of Treg cells and Th17 cells [ 33 ]. Our recent study has demonstrated that the conditioned medium or the secretome derived from human amniotic epithelial cells (AEC-SC) attenuates experimental allergic conjunctivitis in mice [ 34 ]. However, the protective effect of secretome or paracrine factor derived from stem cells on psoriasis has not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

Topical administration of the secretome derived from human amniotic epithelial cells ameliorates psoriasis-like skin lesions in mice

et al. 2022

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Background Psoriasis is a chronic inflammatory skin disease. Tissue stem cells have exhibited a therapeutic effect on psoriatic mice. However, the therapeutic effect of topical administration of the secretome derived from tissue stem cells on psoriasis has not been reported. Methods The secretome from human amniotic epithelial cells (AEC-SC) and human umbilical cord mesenchymal stem cells (UMSC-SC) was topically administrated on the back of imiquimod-induced psoriasis-like mice. Subsequently, we observed the skin lesions and skin inflammation of psoriasis-like mice. Next, we further analyzed the paracrine factors in AEC-SC and UMSC-SC by protein chips. Lastly, the effect of the crucial paracrine factor was investigated by imiquimod-induced psoriasis-like mice. Results We found that AEC-SC had a better therapeutic effect on attenuating psoriasis-like skin lesions including skin scales, skin redness and skin thickness than UMSC-SC, and it had a better regulatory effect on keratinocyte hyperproliferation and altered differentiation. Thus, we focused on AEC-SC. Further study showed that AEC-SC reduced the infiltration of neutrophils and interleukin-17-producing T cells. Next, the analysis of AEC-SC with protein chip revealed that the levels of anti-inflammatory factor interleukin-1 receptor antagonist (IL-1ra) were much higher in AEC-SC compared to that in UMSC-SC. More importantly, the beneficial effect of AEC-SC on psoriasis-like skin lesions and skin inflammation of mice were significantly impaired when neutralizing with IL-1ra antibody, while the recombinant human IL-1ra showed a less protective effect than AEC-SC. Conclusions The present study demonstrated that AEC-SC could efficiently ameliorate psoriasis-like skin lesions and skin inflammation and IL-1ra plays an essential role. Therefore, topical administration of AEC-SC may provide a novel strategy for treating psoriasis-like inflammatory skin diseases.

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Conditioned Medium of Human Amniotic Epithelial Cells Alleviates Experimental Allergic Conjunctivitis Mainly by IL-1ra and IL-10

Cited by 10 publications

References 66 publications

Therapeutic Targets in Allergic Conjunctivitis

Therapeutic Targets in Allergic Conjunctivitis

Antiproliferative and apoptotic effects of conditioned medium released from human amniotic epithelial stem cells on breast and cervical cancer cells

Topical administration of the secretome derived from human amniotic epithelial cells ameliorates psoriasis-like skin lesions in mice

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