Conditions Affecting Social Space in &lt;em&gt;Drosophila melanogaster&lt;/em&gt;

McNeil, Alison R.; Jolley, Sam N.; Akinleye, Adesanya A.; Nurilov, Marat; Rouzyi, Zulekha; Milunovich, Austin J; Chambers, Moria C.; Simon, Anne F.

doi:10.3791/53242-v

Cited by 7 publications

(4 citation statements)

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“…The social space assay was performed as previously described [41,42,66]. In short, once flies have settled at their preferred inter-individual distance, a photo was taken (after 20–50 min, depending on their genotype).…”

Section: Methodsmentioning

confidence: 99%

Recovery from social isolation requires dopamine in males, but not the autism-related gene nlg3 in either sex

Yost,

Scott,

Kurbaj

et al. 2024

R. Soc. Open Sci.

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Social isolation causes profound changes in social behaviour in a variety of species. However, the genetic and molecular mechanisms modulating behavioural responses to social isolation and social recovery remain to be elucidated. Here, we quantified the behavioural response of vinegar flies to social isolation using two distinct protocols (social space preference and sociability, the spontaneous tendencies to form groups). We found that social isolation increased social space and reduced sociability. These effects of social isolation were reversible and could be reduced after 3 days of group housing. Flies with a loss of function of neuroligin3 (orthologue of autism-related neuroligin genes) with known increased social space in a socially enriched environment were still able to recover from social isolation. We also show that dopamine (DA) is needed for a response to social isolation and recovery in males but not in females. Furthermore, only in males, DA levels are reduced after isolation and are not recovered after group housing. Finally, in socially enriched flies mutant for neuroligin3 , DA levels are reduced in males, but not in females. We propose a model to explain how DA and neuroligin3 are involved in the behavioural response to social isolation and its recovery in a dynamic and sex-specific manner.

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Section: Methodsmentioning

confidence: 99%

Recovery from social isolation requires dopamine in males, but not the autism-related gene nlg3 in either sex

Yost,

Scott,

Kurbaj

et al. 2024

R. Soc. Open Sci.

View full text Add to dashboard Cite

show abstract

“…The social space assay: Performed as previously described (Simon et al, 2012;McNeil et al, 2015;Yost et al, 2020). In short: once flies have settled at their preferred inter-individual distance, a photo was taken (after 20-50 minutes, depending on their genotype).…”

Section: Methodsmentioning

confidence: 99%

“…The social space assay was conducted under uniform light in the same room between 1:00 PM and 5:00 PM (ZT 4-8) to decrease behavioural variation linked to diel periodicity. The social space assay: Performed as previously described (Simon et al, 2012;McNeil et al, 2015;Yost et al, 2020). In short: once flies have settled at their preferred inter-individual distance, a photo was taken (after 20-50 minutes, depending on their genotype).…”

mentioning

confidence: 99%

Recovery from social isolation requires dopamine in males, but not the autism-related genenlg3in either sex

Yost,

Scott,

Kurbaj

et al. 2023

Preprint

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Social isolation causes profound changes in social behaviour in a variety of species including humans, monkeys, mice, bees, and vinegar flies. However, the genetic and molecular mechanisms modulating behavioural responses to both social isolation and social recovery remain to be elucidated. In this study, we quantified the behavioural response of vinegar flies to social isolation through the use of two distinct protocols, one involving flies’ social space preference and the other assessing flies’ sociability, defined as their spontaneous tendencies to form groups. We found that social isolation increased social space and reduced sociability. These effects of social isolation, however, were reversible and could be reduced after 3 days of group housing. Flies with a loss of function ofneuroligin3(ortholog of autism-relatedneuroligingenes) with known increased social space in a socially enriched environment, were still able to recover from social isolation. Using aUAS-TH-RNAi driven in all neurons, we show that dopamine is important for a response to social isolation and recovery in males but not in females. Furthermore, only in males, dopamine levels are reduced after isolation and are not recovered after group housing. Finally, in socially enriched flies with a loss of function ofneuroligin3, dopamine levels are reduced in males, but not in females. We propose a model to explain how dopamine andneuroligin3are involved in the behavioural response to social isolation and its recovery in a dynamic and sex-specific manner.

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“…The fly density in the chamber, recorded video length and experimental time also varied. Recent researches have shown the chamber size and fly density had a strong effect on fly social network topology [ 31 ] and social distances [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

DVT: a high-throughput analysis pipeline for locomotion and social behavior in adult Drosophila melanogaster

Mi,

Li,

Yang

et al. 2023

Cell Biosci

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Background Drosophila melanogaster is excellent animal model for understanding the molecular basis of human neurological and motor disorders. The experimental conditions and chamber design varied between studies. Moreover, most previously established paradigms focus on fly trace detection algorithm development. A comprehensive understanding on how fly behaves in the chamber is still lacking. Results In this report, we established 74 unique behavior metrics quantifying spatiotemporal characteristics of adult fly locomotion and social behaviors, of which 49 were newly proposed. By the aiding of the developed analysis pipeline, Drosophila video tracking (DVT), we identified siginificantly different patterns of fly behavior confronted with different chamber height, fly density, illumination and experimental time. Meanwhile, three fly strains which are widely used as control lines, Canton-S(CS), w1118 and Oregon-R (OR), were found to exhibit distinct motion explosiveness and exercise endurance. Conclusions We believe the proposed behavior metrics set and pipeline should help identify subtle spatial and temporal differences of drosophila behavior confronted with different environmental factors or gene variants.

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Conditions Affecting Social Space in <em>Drosophila melanogaster</em>

Cited by 7 publications

References 0 publications

Recovery from social isolation requires dopamine in males, but not the autism-related gene nlg3 in either sex

Recovery from social isolation requires dopamine in males, but not the autism-related gene nlg3 in either sex

Recovery from social isolation requires dopamine in males, but not the autism-related genenlg3in either sex

DVT: a high-throughput analysis pipeline for locomotion and social behavior in adult Drosophila melanogaster

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