Conditions Affecting Transformation by Polyoma Virus

Stoker, M. G. P.; Abel, Pamela

doi:10.1101/sqb.1962.027.001.035

Cited by 69 publications

(14 citation statements)

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“…(41) Polyoma induces tumors in various organs of mice, and transforms a small fraction of cells in a clonally derived population of hamster fibroblasts. (42) Cells that were isolated from a transformed colony failed to multiply when added to a contact-inhibited, confluent sheet of uninfected hamster or mouse cells but did multiply on a sparse layer or a bare surface. (41) Growth inhibition of the polyoma cells requires contact with the confluent sheet of non-transformed cells.…”

Section: How Many Mutations Per Normal Cell In Vivomentioning

confidence: 99%

What keeps cells in tissues behaving normally in the face of myriad mutations?

Rubin

2006

BioEssays

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SummaryThe use of a reporter gene in transgenic mice indicates that there are many local mutations and large genomic rearrangements per somatic cell that accumulate with age at different rates per organ and without visible effects. Dissociation of the cells for monolayer culture brings out great heterogeneity of size and loss of function among cells that presumably reflect genetic and epigenetic differences among the cells, but are masked in organized tissue. The regulatory power of a mass of contiguous normal cells is expressed in its capacity to normalize the appearance and growth behavior of solitary homophilic neoplastic cells, and to redirect differentiation of solitary heterophilic stem-like cells. Intimate contact between the interacting cells is required to induce these changes. The normalization of the neoplastic phenotype does not require gap junctional communication between cells, though transdifferentiation might. These varied relationships are manifestations of the unifying biological principle of ''order in the large over heterogeneity in the small''.

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Section: How Many Mutations Per Normal Cell In Vivomentioning

confidence: 99%

What keeps cells in tissues behaving normally in the face of myriad mutations?

Rubin

2006

BioEssays

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“…The transformation process was therefore inefficient in comparison with the polyoma virus + hamster cell system in which I in IO a infective particles or I in IO 5 physical particles may cause transformation (Stoker & Abel, 1962;Macpherson & Montagnier, I964). Further modifications of the conditions of the test might improve the observed rate of transformation.…”

Section: Discussionmentioning

confidence: 99%

“…In an attempt to quantify the transformation process experiments were made by a method based on a polyoma virus transformation assay using feeder layers of Xirradiated mouse embryo cells (Stoker & Abel, 1962 ). Preliminary observations on the growth of transformed cells at low densities indicated that their growth was enhanced by high densities of X-irradiated mouse feeder cells.…”

Section: Quantitative Assay Of Transjbrmationmentioning

confidence: 99%

Transformation of Hamster Cells in vitro by Adenovirus Type 12

Reed

1967

Journal of General Virology

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SUMMARYHamster brain and kidney cells used as secondary cultures were transformed by adenovirus type 12. Transformed brain cells established in serial culture contained adenovirus tumour antigen and produced tumours when injected into hamsters. Conditions under which transformation may be assayed were determined. In such an assay up to 1/20,000 infected cells gave rise to transformed foci. At high virus input the rate of transformation was decreased probably owing to development of cytopathic effect.

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“…This location also suggests that the protein is not involved in mutagenesis in our assay, We made numerous attempts to demonstrate a dose-response curve for transformation by either HSV or HSV DNA. Such a curve would illustrate one-hit kinetics (Stoker & Abel, 1962). However, our efforts were not successful and this suggests to us that the method of transformation is complex and may be dependent upon promotional insertion.…”

Section: Detection Of Hsv Dna Sequences In Cells Transformed By Shearmentioning

confidence: 97%

Herpes Simplex Virus Sequences Involved in the Initiation of Oncogenic Morphological Transformation of Rat Cells Are Not Required for Maintenance of the Transformed State

Cameron

Park

Dutia

et al. 1985

Journal of General Virology

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SUMMARYWe have determined the herpes simplex virus (HSV) type 2 DNA sequences responsible for the initiation of morphological transformation and have investigated the retention and expression of these sequences in morphologically transformed cells and in tumours derived from these cells. All the transformed cells analysed were selected by a focus formation assay and are oncogenic in the inbred host rat. Cloned HindIII and BglII fragments from the HSV-2 genome were assayed for the ability to initiate morphological transformation of rat embryo cells. Only the HindIII a (map units 0.52 to 0-72) and the BgllI n (0'582 to 0.612) clones gave transformed foci. This shows that the BglII n region is responsible for initiation of transformation. Southern blot analysis of DNA extracted from these transformed cells and from turnouts derived from these transformed cells revealed that neither the BgllI n fragment nor fragments of 500 bp mapping within it are detected at the level of one copy per cell and therefore need not be retained in the cell to maintain the oncogenic phenotype. In addition there was no evidence of expression of the HSV-specified ribonucleotide reductase activity which is partially encoded within the BglII n fragment of HSV-2. We also analysed DNA from rat embryo cells transformed by ts mutants of HSV-2 (HG52) or HSV-1 (HFEM or 17) at non-permissive temperature or by virus at supraoptimal temperature or by sheared virus DNA and DNA from tumours derived from lines of these transformed cells. In addition, we cloned both transformed and tumour cell lines and analysed these similarly. In no case could we detect HSV DNA sequences at the level of one copy per cell.

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Conditions Affecting Transformation by Polyoma Virus

Cited by 69 publications

References 0 publications

What keeps cells in tissues behaving normally in the face of myriad mutations?

What keeps cells in tissues behaving normally in the face of myriad mutations?

Transformation of Hamster Cells in vitro by Adenovirus Type 12

Herpes Simplex Virus Sequences Involved in the Initiation of Oncogenic Morphological Transformation of Rat Cells Are Not Required for Maintenance of the Transformed State

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