Conducting scaffolds for liver tissue engineering

Rad, Armin Tahmasbi; Ali, Naushad; Kotturi, Hari; Yazdimamaghani, Mostafa; Smay, James E.; Vashaee, Daryoosh; Tayebi, Lobat

doi:10.1002/jbm.a.35080

Cited by 61 publications

(40 citation statements)

References 70 publications

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“…143 PEDOT has also been explored as a scaffold material for nerve, bone, 144 and liver tissue engineering. 145 The bulk of the neural tissue engineering research surrounding PEDOT has been associated with its use in electrodes for nerve growth. Unlike PANI, PEDOT is soluble in a wide range of organic solvents and allows chemical modification, and makes it attractive for scaffolding applications.…”

Section: Conductive Polymers For Nerve Growth Conduitsmentioning

confidence: 99%

Peripheral Nerve Regeneration Strategies: Electrically Stimulating Polymer Based Nerve Growth Conduits

Anderson

Shelke

Manoukian

et al. 2015

Crit Rev Biomed Eng

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Treatment of large peripheral nerve damages ranges from the use of an autologous nerve graft to a synthetic nerve growth conduit. Biological grafts, in spite of many merits, show several limitations in terms of availability and donor site morbidity, and outcomes are suboptimal due to fascicle mismatch, scarring, and fibrosis. Tissue engineered nerve graft substitutes utilize polymeric conduits in conjunction with cues both chemical and physical, cells alone and or in combination. The chemical and physical cues delivered through polymeric conduits play an important role and drive tissue regeneration. Electrical stimulation (ES) has been applied toward the repair and regeneration of various tissues such as muscle, tendon, nerve, and articular tissue both in laboratory and clinical settings. The underlying mechanisms that regulate cellular activities such as cell adhesion, proliferation, cell migration, protein production, and tissue regeneration following ES is not fully understood. Polymeric constructs that can carry the electrical stimulation along the length of the scaffold have been developed and characterized for possible nerve regeneration applications. We discuss the use of electrically conductive polymers and associated cell interaction, biocompatibility, tissue regeneration, and recent basic research for nerve regeneration. In conclusion, a multifunctional combinatorial device comprised of biomaterial, structural, functional, cellular, and molecular aspects may be the best way forward for effective peripheral nerve regeneration.

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Section: Conductive Polymers For Nerve Growth Conduitsmentioning

confidence: 99%

Peripheral Nerve Regeneration Strategies: Electrically Stimulating Polymer Based Nerve Growth Conduits

Anderson

Shelke

Manoukian

et al. 2015

Crit Rev Biomed Eng

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“…Alternatively, other theories exist suggesting enhanced cellular communication via manipulation of a membrane potential on conductive scaffold blends of hyaluronic acid-3, 4-ethylenedioxythiophene (PEDOT)-collagen-I-gelatin-chitosan [76]. The depolarization of the neuronal cell membrane is required for initiation of action potential and it is plausible the SWCNT-hydrogel may also enhance cellular communication.…”

Section: Discussionmentioning

confidence: 99%

Robust neurite extension following exogenous electrical stimulation within single walled carbon nanotube-composite hydrogels

et al. 2016

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The use of exogenous electrical stimulation to promote nerve regeneration has achieved only limited success. Conditions impeding optimized outgrowth may arise from inadequate stimulus presentation due to differences in injury geometry or signal attenuation. Implantation of an electrically-conductive biomaterial may mitigate this attenuation and provide a more reproducible signal. In this study, a conductive nanofiller (single-walled carbon nanotubes [SWCNT]) was selected as one possible material to manipulate the bulk electrical properties of a collagen type I-10% Matrigel™ composite hydrogel. Neurite outgrowth within hydrogels (SWCNT or nanofiller-free controls) was characterized to determine if: 1) nanofillers influence neurite extension and 2) electrical stimulation of the nanofiller composite hydrogel enhances neurite outgrowth. Increased SWCNT loading (10–100-μg/ml) resulted in greater bulk conductivity (up to 1.7-fold) with no significant changes to elastic modulus. Neurite outgrowth increased 3.3-fold in 20-μg/mL SWCNT loaded biomaterials relative to the nanofiller-free control. Electrical stimulation promoted greater outgrowth (2.9-fold) within SWCNT-free control. The concurrent presentation of electrical stimulation and SWCNT-loaded biomaterials resulted in a 7.0-fold increase in outgrowth relative to the unstimulated, nanofiller-free controls. Local glia residing within the DRG likely contribute, in part, to the observed increases in outgrowth; but it is unknown which specific nanofiller properties influence neurite extension. Characterization of neuronal behavior in model systems, such as those described here, will aid the rational development of biomaterials as well as the appropriate delivery of electrical stimuli to support nerve repair.

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“…Human primary hepatocytes. Human primary hepatocytes (hPHs) are one of the most matured liver cell sources; while they comprise 60% of the liver volume and have a high proliferation capacity in vivo, 20,28 in vitro utilization of these cells leads to losing their specified functions, which is a cause of considerable difference between their native environment and the laboratory simulations. As a result, they dedifferentiate in traditional cell cultures.…”

Section: Cellsmentioning

confidence: 99%

Liver Tissue Engineering as an Emerging Alternative for Liver Disease Treatment

Mirdamadi

Kalhori

Zakeri

et al. 2020

Tissue Engineering Part B: Reviews

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Chronic liver diseases affect thousands of lives throughout the world every year. The shortage of liver donors for transplantation has been the main driving force to employ alternative methods such as liver tissue engineering (LTE) in fabricating a three-dimensional transplantable liver tissue or enhancing cell delivery techniques alleviating the need for liver donors. LTE consists of three components, cells, ECM (extracellular matrix), and signaling molecules, which we discuss the first and second. The three most common cell sources used in LTE are human and animal primary hepatocytes, and stem cells for different applications. Two major categories of ECM are used to mimic the microenvironment of these cells, named scaffolds and microbeads. Scaffolds have been made by numerous methods with a wide range of synthetic and natural biomaterials. Cell encapsulation has also been utilized by many polymeric biomaterials. To investigate their functions, many properties have been discussed in the literature, such as biochemical, geometrical, and mechanical properties, in both of these categories. Overall, LTE shows excellent potential in assisting hepatic disorders. However, some challenges exist that prevent the practical use of it clinically, making LTE an ongoing research subject in the scientific society.

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Conducting scaffolds for liver tissue engineering

Cited by 61 publications

References 70 publications

Peripheral Nerve Regeneration Strategies: Electrically Stimulating Polymer Based Nerve Growth Conduits

Peripheral Nerve Regeneration Strategies: Electrically Stimulating Polymer Based Nerve Growth Conduits

Robust neurite extension following exogenous electrical stimulation within single walled carbon nanotube-composite hydrogels

Liver Tissue Engineering as an Emerging Alternative for Liver Disease Treatment

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