Conductive hydrogel based on chitosan-aniline pentamer/gelatin/agarose significantly promoted motor neuron-like cells differentiation of human olfactory ecto-mesenchymal stem cells

Bagher, Zohreh; Atoufi, Zhaleh; Alizadeh, Rafieh; Farhadi, Mohammad; Zarrintaj, Payam; Moroni, Lorenzo; Setayeshmehr, Mohsen; Komeili, Ali; Kamrava, Seyed Kamran

doi:10.1016/j.msec.2019.03.068

Cited by 95 publications

(41 citation statements)

References 74 publications

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“…We agree with other groups suggesting using a cell carrier matrix to avoid immediate host rejection [26,28]. In addition to hydrogels , silk fibroin biomaterials , chitosan-gelatin-agarose or poly-caprolactone, an autologous fibrin matrix is a good candidate for cell transplantations [34,[69][70][71][72][73][74][75][76][77][78][79][80]. In this study, we evaluated the loading of a plasma-clot matrix with the purified OSCs for future treatment of SCI.…”

Section: Discussionsupporting

confidence: 57%

Olfactory Stem Cells for the Treatment of Spinal Cord Injury: Isolation, Purification and Behaviour in a Plasma Clot Matrix

Rövekamp¹,

Volkenstein²,

Minovi³

et al. 2020

RGM

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Cell therapies represent promising strategies to improve neurological functions after spinal cord injury (SCI). Olfactory mucosa (OM) might be an attractive source of multipotent cells for neuroregeneration because olfactory stem cells (OSCs) are resident. The regenerative capacity of OSCs has been demonstrated in animal models and some clinical case reports. Up to now, there are no standard methods for purification, characterization, and delivery of OSCs to the injury site. However, purification and characterization of the grafted cells are prerequisites for clinical use to ensure maximum safety for the patients. In this study, we isolated and purified OSCs from human OM using the neurosphere assay. Subsequently, the cells were characterized, and the behavior of purified OSCs in a plasma clot was investigated. Our study demonstrated that isolated cells from OM form neurospheres, which cells are positive for CD105 (98%) and CD90 (99%) and negative for Epcam (<1%) and MUC5AC (<1%). Purified OSCs were positive for Nestin, CD44 as well as GFAP and showed a lack of CD34 and CD45 expression. OSCs differentiated into neuron-like cells expressing ß-III tubulin. However, differentiation into adipocytes, chondrocytes or osteoblast could not be observed. In addition, OSCs stayed viable and were able to proliferate within the plasma clot. These results highlight OSC as a candidate for autologous transplantation in combination with the plasma clot as a cell carrier in SCI and neurodegenerative disease.

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Section: Discussionsupporting

confidence: 57%

Olfactory Stem Cells for the Treatment of Spinal Cord Injury: Isolation, Purification and Behaviour in a Plasma Clot Matrix

Rövekamp¹,

Volkenstein²,

Minovi³

et al. 2020

RGM

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“…Therefore, the mechanical behavior of the intended material should match with its nearby cartilage in order to keep its function [10,11]. Biopolymers such as chitosan [12,13], alginate [14,15], gelatin [16,17], and agarose [18] have been widely used for tissue engineering [19,20] because of their proper biocompatibility, controllable degradability, and tissue-mimicking performance.…”

Section: Introductionmentioning

confidence: 99%

Agarose-Based Biomaterials: Opportunities and Challenges in Cartilage Tissue Engineering

et al. 2020

Self Cite

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The lack of adequate blood/lymphatic vessels as well as low-potential articular cartilage regeneration underlines the necessity to search for alternative biomaterials. Owing to their unique features, such as reversible thermogelling behavior and tissue-like mechanical behavior, agarose-based biomaterials have played a key role in cartilage tissue repair. Accordingly, the need for fabricating novel highly efficient injectable agarose-based biomaterials as hydrogels for restoration of injured cartilage tissue has been recognized. In this review, the resources and conspicuous properties of the agarose-based biomaterials were reviewed. First, different types of signals together with their functionalities in the maintenance of cartilage homeostasis were explained. Then, various cellular signaling pathways and their significant role in cartilage tissue engineering were overviewed. Next, the molecular structure and its gelling behavior have been discussed. Eventually, the latest advancements, the lingering challenges, and future ahead of agarose derivatives from the cartilage regeneration perspective have been discussed.

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“…3D cultures embedded in hydrogels represent a challenging opportunity to advance in tissue engineering and 3D in vitro functional models (Ashammakhi et al, 2019 ). The advent of new technologies, such as 3D printing and bioprinting, allows the production of artificial 3D cell microenvironments, provided that a wide range of printable hydrogels are available (Moroni et al, 2018 ; Bagher et al, 2019 ). Such hydrogels must be biocompatible and able to provide 3D scaffolds with the appropriate structural and chemical features, such as stiffness, viscosity, and capacity to interact with cells providing them with the required biological signals to address their fate (Ooi et al, 2017 ; Neves et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Design and Synthesis of Chitosan—Gelatin Hybrid Hydrogels for 3D Printable in vitro Models

et al. 2020

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The development of 3D printable hydrogels based on the crosslinking between chitosan and gelatin is proposed. Chitosan and gelatin were both functionalized with methyl furan groups. Chemical modification was performed by reductive amination with methyl furfural involving the lysine residues of gelatin and the amino groups of chitosan to generate hydrogels with tailored properties. The methyl furan residues present in both polymers were exploited for efficient crosslinking via Diels-Alder ligation with PEG-Star-maleimide under cell-compatible conditions. The obtained chitosan-gelatin hybrid was employed to formulate hydrogels and 3D printable biopolymers and its processability and biocompatibility were preliminarily investigated.

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Conductive hydrogel based on chitosan-aniline pentamer/gelatin/agarose significantly promoted motor neuron-like cells differentiation of human olfactory ecto-mesenchymal stem cells

Abstract: Conductive hydrogel based on chitosan-aniline pentamer/gelatin/agarose significantly promoted motor neuron-like cells differentiation of human olfactory ecto-mesenchymal stem cells.

Cited by 95 publications

References 74 publications

Olfactory Stem Cells for the Treatment of Spinal Cord Injury: Isolation, Purification and Behaviour in a Plasma Clot Matrix

Olfactory Stem Cells for the Treatment of Spinal Cord Injury: Isolation, Purification and Behaviour in a Plasma Clot Matrix

Agarose-Based Biomaterials: Opportunities and Challenges in Cartilage Tissue Engineering

Design and Synthesis of Chitosan—Gelatin Hybrid Hydrogels for 3D Printable in vitro Models

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