Conductive Thread-Based Immunosensor for Pandemic Influenza A (H1N1) Virus Detection

Abstract

Infectious agents such as viruses pose significant threats to human health, being transmitted via direct contact as well as airborne transmission without direct contact, thus requiring rapid detection to prevent the spread of infectious diseases. In this study, we developed a conductive thread-based immunosensor (CT-IS), a biosensor to easily detect the presence of airborne viruses. CT-IS utilizes an antibody that specifically recognizes the HA protein of the pandemic influenza A (pH1N1) virus, which is incorporated into the conductive thread. The antigen–antibody interaction results in increased strain on the conductive thread in the presence of the pH1N1 virus, resulting in increased electrical resistance of the CT-IS. We evaluated the performance of this sensor using the HA protein and the pH1N1 virus, in addition to samples from patients infected with the pH1N1 virus. We observed a significant change in resistance in the pH1N1-infected patient samples (positive: n = 11, negative: n = 9), whereas negligible change was observed in the control samples (patients not infected with the pH1N1 virus; negative). Hence, the CT-IS is a lightweight fiber-type sensor that can be used as a wearable biosensor by combining it with textiles, to detect the pH1N1 virus in a person’s vicinity.

Development of disposable electrode for the detection of mosquito‐borne viruses

“…The electrochemical-based biosensor has recently been advanced, providing a high signal-to-noise in analyte detection. This is accomplished by the developing various nanomaterials as the signal transducer [25][26][27][28] with its signal amplification strategy. [29,30] However, the complex composition of the electrode, starting from the signal transducer, advanced nanomaterial, and bioconjugation, mostly fails to be adapted for POC diagnostic tools for on-site biosensor.…”

Quenched Electrochemical Biosensor Based on Adsorption Between GO and Aptamer for the Detection of ERα

“…16 In electrochemical biosensors, antigen-antibody reaction is often used in the detection of various biomolecules due to its strong specificity and high sensitivity. 17,18 However, antibodies are usually expensive and susceptible to environmental denaturation. 19 Aptamer is a DNA or RNA sequence screened in vitro through systematic evolution of ligands by exponential enrichment (SELEX), which can be used as a probe to specifically combine with the target to achieve the capture of the target.…”