Cone contributions to the photopic spectral sensitivity 
of the zebrafish ERG

Hughes, A.D.; Saszik, Shannon; Bilotta, Joseph; DeMarco, Paul J.; Patterson, Warren F.

doi:10.1017/s095252389815602x

Cited by 80 publications

(161 citation statements)

References 25 publications

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“…Its development closely follows the morphologic maturation of the retina. By 5 dpf, electroretinograms (ERG) containing all the components of a typical vertebrate ERG can be recorded (Branchek, 1984;Brockerhoff et al, 1995;Hughes et al, 1998;Saszik et al, 1999;Bilotta et al, 2001;Seeliger et al, 2002).…”

Section: Maturation Of the Visual System And Emergence Of Form Visionmentioning

confidence: 99%

Behavioral genetic approaches to visual system development and function in zebrafish

2002

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ABSTRACT:The zebrafish is a recent vertebrate model system that shows great potential for a genetic analysis of behavior. Early development is extraordinarily rapid, so that larvae already display a range of behaviors 5 days after fertilization. In particular the visual system develops precociously, supporting a number of visually mediated behaviors in the larva. This provides the opportunity to use these visually mediated behaviors to screen chemically mutagenized strains for defects in vision. Larval optokinetic and optomotor responses have already been successfully employed to screen for mutant strains with defects in the visual system. In the adult zebrafish a visually mediated escape response has proved useful for screening for dominant mutations of the visual system. Here, I summarize visually mediated behaviors of both larval and adult zebrafish and their applicability for genetic screens, and present, the approaches and results of visual behavior carried out to date.

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Section: Maturation Of the Visual System And Emergence Of Form Visionmentioning

confidence: 99%

Behavioral genetic approaches to visual system development and function in zebrafish

2002

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“…To derive a spectral sensitivity function, the reciprocal of the log stimulus irradiance that produced a 50 V criterion response was determined for each stimulus wavelength . To derive sensitivity values, log irradiance vs. log response functions were generated for each stimulus wavelength and linear regression was used to interpolate to find the stimulus irradiance that produced the criterion response (Hughes et al, 1998). The 50-V criterion response was chosen because it fell within the linear portion of the log irra- Fig.…”

Section: Resultsmentioning

confidence: 99%

“…dures have been used to derive the photoreceptor contributions to the spectral sensitivity functions of lightadapted and dark-adapted adult zebrafish ERG (Hughes et al, 1998;. Table 1 shows the resulting weights from the model incorporating all four cone types and rods across ages.…”

Section: Resultsmentioning

confidence: 99%

“…Recent physiological work has examined adult zebrafish spectral sensitivity under both light-adapted (Hughes et al, 1998) and dark-adapted conditions. Under photopic conditions, the spectral sensitivity derived from the b-wave response of the ERG appears to receive contributions from the four known cone types (U-, S-, M-, and Lcones; max ϭ 362, 415, 480, and 570 nm, respectively (Robinson et al, 1993) and appears to be the result of both nonopponent and opponent interactions (Hughes et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…Under photopic conditions, the spectral sensitivity derived from the b-wave response of the ERG appears to receive contributions from the four known cone types (U-, S-, M-, and Lcones; max ϭ 362, 415, 480, and 570 nm, respectively (Robinson et al, 1993) and appears to be the result of both nonopponent and opponent interactions (Hughes et al, 1998). Under dark-adapted conditions, the spectral sensitivity function of the adult zebrafish appears to receive input from rod photoreceptors containing rhodopsin as well as from U-cones .…”

Section: Introductionmentioning

confidence: 99%

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Rod contributions to the electroretinogram of the dark‐adapted developing zebrafish

Bilotta

Saszik

Sutherland

2001

Developmental Dynamics

Self Cite

131

119

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Anatomical studies of the developing zebrafish retina have shown that rods approach maturity at about 15 days postfertilization (dpf). Past work has examined the photopic spectral sensitivity function of the developing zebrafish, but not spectral sensitivity under darkadapted conditions. This study examined rod contributions to the dark-adapted spectral sensitivity function of the ERG b-wave component in developing zebrafish. ERG responses to stimuli of various wavelengths and irradiances were obtained from dark-adapted fish at 6-8, 13-15, 21-24, and 27-29 dpf. The results show that dark-adapted spectral sensitivity varied with age. Spectral sensitivity functions of the 6-8 and 13-15 dpf groups appeared to be cone dominated and contained little or no rod contributions. Spectral sensitivity functions of the 21-24 and 27-29 dpf groups appeared to have both rod and cone contributions. Even at the oldest age group tested, the darkadapted spectral sensitivity function did not match the adult function. Thus, consistent with anatomical findings, the rod contributions to the ERG spectral sensitivity function appear to develop with age; however, these contributions are still not adult-like by 29 dpf, which is contrary to anatomical work. These results illustrate that the zebrafish is an excellent model for visual development.

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Ontogeny of ultraviolet‐sensitive cones in the retina of rainbow trout (Oncorhynchus mykiss)

Allison

Dann

Helvik

et al. 2003

J of Comparative Neurology

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In order to facilitate emerging models of retinal development, we developed electroretinogram and in situ hybridization protocols to examine the ontogeny of photoreceptors in the retina of a land-locked salmonid, the rainbow trout (Oncorhynchus mykiss). We cloned cDNA fragments corresponding to the rod opsin and each of the four cone opsin gene families, which we utilized to produce riboprobes. We established the specificity of the in situ hybridization protocol by examining subcellular signal localization and through double-labeling experiments. We confirm the assumption that the accessory corner cones in the square mosaic are the ultraviolet wavelength-sensitive (UVS) cone photoreceptor (i.e., they express an SWS1 opsin) and observed UVS cones throughout the retina of small trout. Larger fish have a decrease in sensitivity to short wavelength light stimuli and the distribution of UVS cones in the mature retina is limited to the dorsal-temporal quadrant. These larger fish also possess differentiated UVS cones in the peripheral germinal zone (PGZ), including within areas peripheral to mature retina lacking UVS cones. These data are consistent with the loss of putative UVS cones from the PGZ of a migratory salmonid of another genus, and thus the disappearance of UVS cones appears to be general to the Family Salmonidae, regardless of life history strategy. The generation, differentiation, and subsequent loss of UVS cones in the smolt PGZ is a dramatic example of the supposition that the mechanisms of PGZ development recapitulate the retinal embryogenesis of that species.

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Cone contributions to the photopic spectral sensitivity of the zebrafish ERG

Cited by 80 publications

References 25 publications

Behavioral genetic approaches to visual system development and function in zebrafish

Behavioral genetic approaches to visual system development and function in zebrafish

Rod contributions to the electroretinogram of the dark‐adapted developing zebrafish

Ontogeny of ultraviolet‐sensitive cones in the retina of rainbow trout (Oncorhynchus mykiss)

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