Conferring antioxidant capacity to cellulose based materials by using enzymatically-modified products

Abstract

Abstract:A new, industrially feasible method for conferring cellulosic substrates antioxidant properties by using enzymatic products was developed. The method allows cellulose surfaces such as those of paper sheets (PS) to be conferred antioxidant capacity by using a functionalization solution (FS) obtained from an enzymatic reaction. Various laccases and different phenolic compounds (PhC) potentially possessing antioxidant action were used to prepare the functionalization solutions. Antioxidant capacity was assessed by using UV spectrophotometry to monitor the inhibition of ABTS radical cation (ABTS •+ ) in the presence of antioxidants. Based on the results, enzymatic modification of the phenolic compound in the functionalization solution increases the bonding strength of its components to cellulosic materials. Evidences on the grafting of the FS onto the cellulosic sheets, and changes in the chemical structure of the resulting oxidized form of PhCs were assessed by using ATR-FTIR. The level of antioxidant capacity achieved depends on the enzyme type, the chemical structure of the compounds in FS, and the presence of lignosulfonates (SL) in it. The potential of the proposed method for conferring antioxidant properties to cellulose-based materials by surface application of a product obtained from an enzymatic reaction is demonstrated here for the first time.

Sustainability in e-commerce packaging: A review

“…As shown by existing literature, cellulose-based materials have a very low environmental impact relative to other choices ( Suhas et al, 2016 ). Cellulose is one of the most naturally abundant materials ( Abitbol et al, 2016 ; Bharimalla, 2017 ; Klemm, 2018 ; Li, 2015 ; Nechyporchuk et al, 2016 ; Osong et al, 2016 ; Perepelkin, 2004 ; Rajinipriya, 2018 ; Tayeb, 2018 ; Vilarinho et al, 2018 ; Yang et al, 2019 ) and one of the most important renewable materials ( Brinchi et al, 2013 ; Cusola, 2015 ; Hischier et al, 2005 ; Osong et al, 2016 ; Piselli, 2016 ).…”

Antioxidant activity of xylooligosaccharides produced from glucuronoxylan by Xyn10A and Xyn30D xylanases and eucalyptus autohydrolysates

“…The antioxidant activity was assessed by a procedure consisting in the quantification of the ABTS •+ radical decoloration described by several authors (Cusola, Valls, Vidal, & Roncero, 2015;Re et al, 1999; with some modifications. The method consists in the addition of the antioxidant compound to a pre-formed ABTS •+ radical solution and quantifying the remaining ABTS •+ after a fixed time period, by means of UV spectrophotometry.…”

“…At a concentration of 200 µg xylose reducing equivalents mL -1 the antioxidant activity was around 80% in XOS_30 and 10% in XOS_10. The antioxidant activity of a compound can be compared to that of Trolox, obtaining the TEAC (trolox equivalent antioxidant activity) value (Cusola et al, 2015;Re et al, 1999). However, this value is determined according to the molar concentration of the compound.…”

Lignin Particles for Multifunctional Membranes, Antioxidative Microfiltration, Patterning, and 3D Structuring

“…The antioxidant capacity of membranes was measured by using a variation of the method used by Serpen et. al., 42 by quantification of the ABTS •+ radical inhibition and as previously reported 43,44 . First, the ABTS radical cation was generated by reacting potassium persulfate with the ABTS salt.…”

“…Following, absorbance measures were performed by taking 1 mL of the supernatant and placing it into a methacrylate cuvette. The absorbance value of each sample at 752 nm was monitored every minute up to 6 min, or until stabilization 44 by UV-Vis spectrophotometry, using a Thermo Scientific Evolution 600 instrument. The reduction in ABTS •+ concentration, induced by a certain amount of antioxidant, was related to that of Trolox (a standard reference antioxidant compound), via a pre-made calibration curve.…”