2022
DOI: 10.1101/2022.03.19.484972
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Configurable Compartmentation Enables In Vitro Reconstitution of Sustained Synthetic Biology Systems

Abstract: The compartmentalized and communicative nature of biological cells contributes to the complexity and endurance of living organisms. We present a general compartmentalization strategy for in vitro systems that inherits the passive transport phenomenon of biology. The strategy incorporates layered, micrometer-sized, hydrogel-based compartments featuring configurability in composition, functionality and selective permeability of biomolecules. We demonstrated the utility of the strategy by reconstituting a compart… Show more

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“…67 Similarly, bacterial microcompartments have been employed for propanediol utilization. 68 Synthetic biology toolkits have also been used to perform in vitro compartmentalization 69 and light-based synthetic organelle assembly 70 to cluster enzymes together. Currently, most of the work in the field primarily focuses on targeting pathways to compartments.…”
Section: Knowledge-guided Designmentioning
confidence: 99%
“…67 Similarly, bacterial microcompartments have been employed for propanediol utilization. 68 Synthetic biology toolkits have also been used to perform in vitro compartmentalization 69 and light-based synthetic organelle assembly 70 to cluster enzymes together. Currently, most of the work in the field primarily focuses on targeting pathways to compartments.…”
Section: Knowledge-guided Designmentioning
confidence: 99%