Confirmation of aflatoxins B1 and B2 in peanuts by gas chromatography/mass spectrometry/selected ion monitoring

Rosén, Robert; Rosen, Joseph D.; Diprossimo, Vincent P

doi:10.1021/jf00122a025

Cited by 19 publications

(4 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Without chemical derivatization, experiments required an on-column mass of 10 ng for detection and no food samples were analyzed. Two similar studies were reported by Trucksess et al [251] and Rosen et al [298], in which aflatoxins were determined by the MS detector coupled with the GC but separated on different GC capillary columns. Goto et al [299,300] reported the analysis of aflatoxins using GC-FID.…”

Section: Gas Chromatographysupporting

confidence: 59%

A Review: Sample Preparation and Chromatographic Technologies for Detection of Aflatoxins in Foods

Zhang

Banerjee

2020

Toxins

102

View full text Add to dashboard Cite

As a class of mycotoxins with regulatory and public health significance, aflatoxins (e.g., aflatoxin B1, B2, G1 and G2) have attracted unparalleled attention from government, academia and industry due to their chronic and acute toxicity. Aflatoxins are secondary metabolites of various Aspergillus species, which are ubiquitous in the environment and can grow on a variety of crops whereby accumulation is impacted by climate influences. Consumption of foods and feeds contaminated by aflatoxins are hazardous to human and animal health, hence the detection and quantification of aflatoxins in foods and feeds is a priority from the viewpoint of food safety. Since the first purification and identification of aflatoxins from feeds in the 1960s, there have been continuous efforts to develop sensitive and rapid methods for the determination of aflatoxins. This review aims to provide a comprehensive overview on advances in aflatoxins analysis and highlights the importance of sample pretreatments, homogenization and various cleanup strategies used in the determination of aflatoxins. The use of liquid-liquid extraction (LLE), supercritical fluid extraction (SFE), solid phase extraction (SPE) and immunoaffinity column clean-up (IAC) and dilute and shoot for enhancing extraction efficiency and clean-up are discussed. Furthermore, the analytical techniques such as gas chromatography (GC), liquid chromatography (LC), mass spectrometry (MS), capillary electrophoresis (CE) and thin-layer chromatography (TLC) are compared in terms of identification, quantitation and throughput. Lastly, with the emergence of new techniques, the review culminates with prospects of promising technologies for aflatoxin analysis in the foreseeable future.

show abstract

Section: Gas Chromatographysupporting

confidence: 59%

A Review: Sample Preparation and Chromatographic Technologies for Detection of Aflatoxins in Foods

Zhang

Banerjee

2020

Toxins

102

View full text Add to dashboard Cite

show abstract

“…GC analysis of AFs became feasible with the arrival of fused silica capillary columns. In 1984, Rosen et al were the first to use a GC-MS approach as a confirmatory method for AFB 1 and AFB 2 in peanuts [155]. Unfortunately, the method was not developed for the quantitation of total AFs.…”

Section: Gas Chromatography (Gc)mentioning

confidence: 99%

Current Developments of Analytical Methodologies for Aflatoxins’ Determination in Food during the Last Decade (2013–2022), with a Particular Focus on Nuts and Nut Products

Schincaglia

Aspromonte

Franchina

et al. 2023

Foods

View full text Add to dashboard Cite

This review aims to provide a clear overview of the most important analytical development in aflatoxins analysis during the last decade (2013–2022) with a particular focus on nuts and nuts-related products. Aflatoxins (AFs), a group of mycotoxins produced mainly by certain strains of the genus Aspergillus fungi, are known to impose a serious threat to human health. Indeed, AFs are considered carcinogenic to humans, group 1, by the International Agency for Research on Cancer (IARC). Since these toxins can be found in different food commodities, food control organizations worldwide impose maximum levels of AFs for commodities affected by this threat. Thus, they represent a cumbersome issue in terms of quality control, analytical result reliability, and economical losses. It is, therefore, mandatory for food industries to perform analysis on potentially contaminated commodities before the trade. A full perspective of the whole analytical workflow, considering each crucial step during AFs investigation, namely sampling, sample preparation, separation, and detection, will be presented to the reader, focusing on the main challenges related to the topic. A discussion will be primarily held regarding sample preparation methodologies such as partitioning, solid phase extraction (SPE), and immunoaffinity (IA) related methods. This will be followed by an overview of the leading analytical techniques for the detection of aflatoxins, in particular liquid chromatography (LC) coupled to a fluorescence detector (FLD) and/or mass spectrometry (MS). Moreover, the focus on the analytical procedure will not be specific only to traditional methodologies, such as LC, but also to new direct approaches based on imaging and the ability to detect AFs, reducing the need for sample preparation and separative techniques.

show abstract

“…The analytical techniques used to determine AFs must be highly sensitive and accurate so trace AFs in the agro‐products can still be detected. Currently, the main analytical techniques used to detect AFs are chromatography combined with spectroscopy such as thin‐layer chromatography (TLC), gas chromatography (GC) and high‐performance liquid chromatography (HPLC) equipped with various spectroscopic detection systems …”

mentioning

confidence: 99%

“…[7] The analytical techniques used to determine AFs must be highly sensitive and accurate so trace AFs in the agro-products can still be detected. Currently, the main analytical techniques used to detect AFs are chromatography combined with spectroscopy such as thin-layer chromatography (TLC), [8][9][10] gas chromatography (GC) [11] and high-performance liquid chromatography (HPLC) equipped with various spectroscopic detection systems. [12,13] Among these techniques, gas chromatography/mass spectrometry (GC/MS) and high -performance liquid chromatography/mass spectrometry (HPLC/MS) are the most often used techniques to detect AFs due to their extremely high sensitivity and accuracy.…”

mentioning

confidence: 99%

Rapid characterization of trace aflatoxin B₁ in groundnuts, wheat and maize by dispersive liquid‐liquid microextraction followed by direct electrospray probe tandem mass spectrometry

Satarpai

Siripinyanond

et al. 2017

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

show abstract

Confirmation of aflatoxins B1 and B2 in peanuts by gas chromatography/mass spectrometry/selected ion monitoring

Cited by 19 publications

References 4 publications

A Review: Sample Preparation and Chromatographic Technologies for Detection of Aflatoxins in Foods

A Review: Sample Preparation and Chromatographic Technologies for Detection of Aflatoxins in Foods

Current Developments of Analytical Methodologies for Aflatoxins’ Determination in Food during the Last Decade (2013–2022), with a Particular Focus on Nuts and Nut Products

Rapid characterization of trace aflatoxin B₁ in groundnuts, wheat and maize by dispersive liquid‐liquid microextraction followed by direct electrospray probe tandem mass spectrometry

Contact Info

Product

Resources

About

Confirmation of aflatoxins B1 and B2 in peanuts by gas chromatography/mass spectrometry/selected ion monitoring

Cited by 19 publications

References 4 publications

A Review: Sample Preparation and Chromatographic Technologies for Detection of Aflatoxins in Foods

A Review: Sample Preparation and Chromatographic Technologies for Detection of Aflatoxins in Foods

Current Developments of Analytical Methodologies for Aflatoxins’ Determination in Food during the Last Decade (2013–2022), with a Particular Focus on Nuts and Nut Products

Rapid characterization of trace aflatoxin B1 in groundnuts, wheat and maize by dispersive liquid‐liquid microextraction followed by direct electrospray probe tandem mass spectrometry

Contact Info

Product

Resources

About

Rapid characterization of trace aflatoxin B₁ in groundnuts, wheat and maize by dispersive liquid‐liquid microextraction followed by direct electrospray probe tandem mass spectrometry