Confirmation of biological effects of high dilutions. Effects of submolecular concentrations of histamine and 1-, 3- and 4-methylhistamines on human basophil activation

Inhibition of basophil activation by histamine: a sensitive and reproducible model for the study of the biological activity of high dilutions

“…In contrast to the IgE/CD63 flow cytometric protocol, the two main changes were, apart the use of another activation marker: preparation of the tested leukocytes by a buffy coat (centrifugation of the total blood and separation of the layer of white cells situated between the plasma Inhibition of basophil activation by histamine J Sainte-Laudy and P Belon and the erythrocytes pellet) and the use of the peptide fMLP instead of an anti-IgE for basophil activation. This protocol led to results [47][48][49] confirming results obtained with CD63, the magnitude of histamine 16CH being higher with CD203c (32%, p = 0.004, (Figure 10)) than with CD63. Moreover, if these results were expressed in activation index calculated after linearization of the MFI-CD203c (data are given by the cytometer in log units) the inhibition induced by histamine 16CH and histamine 2CH reached respectively, 78% and 96% These results also showed that the effect of histamine in high dilution was not significant on the IgE marker measured by the MFI-IgE, which was not susprising as IgE downregulation is closely linked to an IgE dependent activation, in contrast of fMLP-induced activation.…”

“…More recently, we demonstrated that among the different methylated histamine derivatives (1-Methyl, 3-Methyl and 4-Methyl histamine) tested at the 16CH dilution, only 16CH 4-Methyl histamine 49 (Figure 11) had a significant effect on fMLP-induced basophil activation. These results showed that inhibition of biological effects on human basophils by high dilutions of histamine was similar to the activity of H2 or H4 agonists and that the effect is specific, as the 1-Methyl and 3-Methyl derivatives had no activity in the same conditions.…”

Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study

“…Controversies over the manual method used to assess basophil activation, along with its time-consuming and subjective nature, prompted adoption of a standardized automated flow cytometry approach [ 12 ], which was able to evaluate the effect of highly diluted compounds by quantitatively following membrane expression of the investigated cells. More recently, similar effects have been confirmed by two independent laboratories [ 13 , 14 ] and by the group of Belon and Sainte-Laudy [ 15 , 16 ], who also report that CD203c is the most suitable marker for this type of effect [ 17 ]. However, the literature in this field is not always consistent, with some experiments showing that highly diluted histamine activates rather than inhibiting the same cells [ 18 ], while another group failed to replicate the reported inhibitory effects of high histamine dilutions [ 19 ].…”

Repetitions of fundamental research models for homeopathically prepared dilutions beyond 10-23: a bibliometric study

“…46,47 B Results on potentized histamine 24 were confirmed by the same working group and by others. [48][49][50][51][52][53][54][55][56][57] Isolated organs B Results on potentized atropa belladonna/atropine sulfate 27 were confirmed by others. [58][59][60] Animals B Consistent multicenter results on potentized thyroxin and frog metamorphosis were obtained 28 and subsequently confirmed by the same working group with potentized thyroxin [61][62][63] and by others with potentized thyroidinum.…”