Confirmation of the Fire Blight QTL of Malus × Robusta 5 on Linkage Group 3

Peil, Andreas; Hanke, Magda‐Viola; Flachowsky, Henryk; Richter, Klaus; Garcia-Libreros, T.; Celton, Jean‐Marc; Gardiner, Susan E.; Horner, M.; Bus, Vincent G. M.

doi:10.17660/actahortic.2008.793.44

Cited by 39 publications

(34 citation statements)

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“…The cultivars in this subset were screened with 12 SSR loci to obtain a data set for estimation of similarity (and putative relatedness) among all the cultivars in general, as well as similarity to 'Cox's Orange Pippin' in particular. Some additional samples, references M. robusta 5, 'Discovery' and 'Prima' (Peil et al 2007(Peil et al , 2008 and 8 cultivars, 'Elise', 'Filippa', 'Goldparmäne', 'Laxton's Superb', 'Mio', 'Norrstack', 'Ribston' and 'Spässerud' that had been inoculated with fire blight for a previous study (Sehic et al 2009) were screened for one of the SSR loci, CH03e03, which has proved to be associated with a fire blight QTL on LG 3 in M. robusta 5 (Peil et al 2007(Peil et al , 2008.…”

Section: Materials For Ssr Analysis and Inoculation Testsmentioning

confidence: 99%

“…Since many cultivars obviously have only one of the markers, some of the cultivars with both markers may carry these on separate chromosomes and therefore may still lack the QTL. One SSR locus, CH03e03, which has previously proved to be associated with a fire blight QTL on LG 3 in M. robusta (Peil et al 2007(Peil et al , 2008, was screened in the 23 cultivars chosen for inoculation tests in the present study, as well as in 8 additional cultivars and three references. We found a total of 10 alleles in this material; 184,185,188,198,200,202,204,206,213 and 217 bp.…”

Section: Dna Marker Datamentioning

confidence: 99%

“…The CH03e03 locus, previously shown to be associated with a fire blight QTL on LG 3 in M. robusta (Peil et al 2007(Peil et al , 2008, was screened in the 23 cultivars chosen for inoculation tests in the present study as well as in eight additional cultivars and three references. As expected, we found the allele denoted as 184 bp only in Malus robusta 5 (184.57 and 184.61).…”

Section: Dna Markers For Lgmentioning

confidence: 99%

“…Considerable differences among apple cultivars in susceptibility to fire blight have been reported, and heritability estimates for field resistance have been determined (Hampson and Sholberg 2008). Major QTLs for fire blight resistance have been found on linkage group (LG) 3 in M. robusta 5 explaining up to 80% of the phenotypic variation (Peil et al 2007(Peil et al , 2008) and on LG 12 in the ornamental cultivar 'Evereste' (possibly a derivative of a wild Malus species) and in M. floribunda clone 821 (Durel et al 2009). Promising sources of fire blight resistance in M. fusca and M. sieversii are also being investigated at present Peil et al 2010;Baumgartner et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of fire blight tolerance in apple based on plant inoculations with Erwinia amylovora and DNA markers

Nybom

Mikiciński

Garkava-Gustavsson

et al. 2011

Trees

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Fire blight (Erwinia amylovora) causes serious damage to pome fruit orchards, and identification of germplasm with heritable disease resistance is therefore crucial. Two dominant SCAR (sequence characterised amplified region) marker alleles (AE10-375 and GE-8019), flanking a previously identified QTL (quantitative trait locus) for resistance to fire blight on 'Fiesta' linkage group 7 in apple cultivars related to 'Cox's Orange Pippin', were screened on 205 apple cultivars. Both marker alleles were present in 22% of the cultivars, indicating presence of the QTL allele for tolerance, and both were lacking in 25%, indicating homozygosity for absence of the QTL tolerance allele. However, 33% had only the marker allele AE10-375, while 20% had only GE-8019, suggesting that some cultivars with the dominant alleles for both of the flanking markers can carry these on separate chromosomes and may lack the QTL allele for tolerance. In 2009 and 2010, terminal shoots of greenhouse-grown grafted trees of 21 cultivars (only 20 in 2010) were inoculated with Erwinia amylovora. 'Idared' (susceptible) and 'Enterprise' (tolerant) were included as controls. Disease severity for each cultivar was expressed as percentage of necrosis in relation to entire length of shoot, and the ranking of cultivars in 2009 and 2010 was compared with a Spearman rank correlation test, P \ 0.01. A relationship between presence of both flanking marker alleles for tolerance and level of fire blight tolerance was confirmed with a Mann-Whitney U-test, P \ 0.01 in 2009, and P \ 0.05 in 2010. A PCO (principal coordinate) analysis based on band profiles obtained with 12 SSR (simple sequence repeat) loci produced three loose clusters, two of which contained known offspring of 'Cox's Orange Pippin', and one with cultivars that were either unrelated or had an unknown origin. Cases where DNA markers did not predict level of fire blight damage as expected, were, however, as common among descendants of 'Cox's Orange Pippin' as among apparently unrelated cultivars. Obviously the 'Fiesta' LG 7 QTL has some predictive value, both for known 'Cox' relatives and others, but more efficient markers would be desirable for marker-assisted selection.

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Section: Materials For Ssr Analysis and Inoculation Testsmentioning

confidence: 99%

Section: Dna Marker Datamentioning

confidence: 99%

Section: Dna Markers For Lgmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of fire blight tolerance in apple based on plant inoculations with Erwinia amylovora and DNA markers

Nybom

Mikiciński

Garkava-Gustavsson

et al. 2011

Trees

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“…The major fire blight QTL has been mapped to linkage group 3 in M. robusta, and explains up to 80% of the phenotypic variation [74] [75]. However, the QTL mapped to linkage group 7 for fire blight resistance in 'Fiesta' (M. domestica) explains up to 46% of the phenotypic variation, and has been tracked back to its parent, 'Cox's Orange Pippin' [76] [77].…”

Section: Resistance Indicesmentioning

confidence: 99%

Excised Leaf Bioassay: Phenotypic Expression of Natural Resistance in Apple against the Obliquebanded Leafroller

Schwarz¹,

Brunner²

2017

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Different approaches were assessed in the development of a bioassay method that would provide data necessary to evaluate resistance of apple genotypes to the obliquebanded leafroller (OBLR) Choristoneura rosaceana (Harris). A leaf disc method used to evaluate the impact of pesticides on leafrollers resulted in high levels of leaf-abandonment of first and second instar larvae. Inserting leaf petioles into water soaked Oasis foam resulted in a significant reduction in overall leaf weight and diminished the appearance of leaf quality. When leaf petioles were inserted into waterfilled Eppendorf vials, leaf weight increased and overall leaf quality appeared to be good. We opted to adopt this latter whole-leaf bioassay method, which though very time consuming, provided a robust assessment of OBLR life history parameters against different apple genotypes. Across all apple genotypes evaluated, male larvae developed faster than females to pupation as well as to adulthood, while females developed faster as pupae and were heavier than male pupae. OBLR larvae reared on leaves from different apple genotypes displayed significant variability in development time, pupal weight, larval survivorship, and number of offspring produced. We utilized three indices to characterize host acceptance, sub-lethal effects, and lethal effects of OBLR to apple genotypes. Our results indicated that 'Lady', 'Viking', and 'Northern Spy' show some of the highest levels of resistance against OBLR, while 'Granny Smith' was quite susceptible to OBLR and could be used in future studies identifying apple genotypes with relative resistance to OBLR.

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