Confirmation of the synteny of the human genes for mannose phosphate isomerase and pyruvate kinase and of their assignment to chromosome 15

Chern, Ching J.; Croce, C.M.

doi:10.1159/000130527

Cited by 16 publications

(6 citation statements)

References 7 publications

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“…Since p58 is a subunit of PKMz, our results confirmed the earlier findings in which PKM z was reported to be on 15q22-qter (15)(16)(17) 15q24-25 raises the possibility that this gene may serve as a useful marker for Tay-Sachs disease. Hexosaminidase A, the enzyme lacking in patients with Tay-Sachs disease, is also located in the same chromosomal region (15@3-24) as the p58 gene (22,23).…”

Section: Discussionsupporting

confidence: 90%

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Chromosomal localization of the gene for a human cytosolic thyroid hormone binding protein homologous to the subunit of pyruvate kinase, subtype M2

Popescu

Cheng

1990

Somat Cell Mol Genet

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Section: Discussionsupporting

confidence: 90%

“…Using a human-mouse hybrid clone, human PKM 2 has been assigned on 15@2-qter (15)(16)(17). However, these previous studies have not assigned its subregional location.…”

Section: Introductionmentioning

confidence: 99%

Chromosomal localization of the gene for a human cytosolic thyroid hormone binding protein homologous to the subunit of pyruvate kinase, subtype M2

Popescu

Cheng

1990

Somat Cell Mol Genet

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“…Other laboratories, using mouse X human or Chinese hamster X human hybrids, have demonstrated the independent segregation of Hex A and B (19,26,28). These and other studies (16)(17)(18)(19) have linked expression of Hex A to chromosome 15. No individual has ever been described in whom Hex A is present in the absence of Hex B. The electrophoretic demonstration of "Hex A"+/Hex B-hybrid clones by several laboratories (19,26,28), including our own, however, is not an anomaly but appears to result from the cell hybridization process itself.…”

Section: Resultsmentioning

confidence: 71%

“…(24). Cells for mannosephosphate isomerase, pyruvate kinase, and glucose-6-phosphate dehydrogenase electrophoresis were prepared as reported before (18).…”

mentioning

confidence: 99%

“…Starch gel electrophoresis was used to identify the human isozymes of mannosephosphate isomerase, pyruvate kinase, and glucose-6-phosphate dehydrogenase in the hybrid cell samples as described (18). Cellulose acetate gel [Cellogel; (17 A and B (,B chain) were prepared as described (14) and purified by beads was similar to that described for antibody against Hex A chain bound to Sepharose beads.…”

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Characterization of heteropolymeric hexosaminidase A in human X mouse hybrid cells.

Chern¹,

Beutler²,

Kühl³

et al. 1976

Proc. Natl. Acad. Sci. U.S.A.

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(19,20) suggested that there was no structural relationship between these two isozymes. We have therefore considered the possibility that the "Hex A" that occurs in mouse X human hybrids in the absence of human Hex B is, in point of fact, not human Hex A. Accordingly, we have investigated the "Hex A" activity that is found in the absence of Hex B in a human X mouse hybrid cell line that contains human chromosome 15 but not human chromosome 5. Immunological studies indicate that this "Hex A" material contains a normal human a subunit and is most probably a hybrid molecule containing human and mouse components. The genetic control of Tay-Sachs and Sandhoff diseases is discussed in the light of our findings. (23) and the genes for mannosephosphate isomerase, pyruvate kinase, and "Hex A" have been assigned to chromosome 15 (16-19). Five of the resulting subclones were analyzed for the continued expression of the human enzyme markers.Electrophoresis. Cells for Hex electrophoresis were grown in McCoy's 5A medium with 10% fetal calf serum added. They were harvested with 0.25% trypsin, centrifuged down, and resuspended in a 0.02 M citrate buffer, pH 5.2. The cell suspension was frozen and thawed five times and centrifuged at 14,500 X g for 15 min. The supernatant was concentrated to dryness by lyophilization. The final samples were dissolved in small volumes of 0.02 M citrate buffer, pH 5.2 (24). Cells for mannosephosphate isomerase, pyruvate kinase, and glucose-6-phosphate dehydrogenase electrophoresis were prepared as reported before (18).Starch gel electrophoresis was used to identify the human isozymes of mannosephosphate isomerase, pyruvate kinase, and glucose-6-phosphate dehydrogenase in the hybrid cell samples as described (18). Cellulose acetate gel [Cellogel; (17 cm X 17 cm X 0.5 mm) Kalex Scientific Co., Manhasset, N.Y.] was used to identify Hex A and B using modifications of the method of Okada and O'Brien (11). The sample solution (approximately 0.4 Al) was applied to the Cellogel with the aid of a multiple sample applicator (Shandon Scientific Co., Inc., Sewickley, Pa.). Electrophoresis was performed at 250 V for 2 hr, and the gel was incubated with 4-methylumbelliferyl-f.-D-glucosami iide (Sigma Chemical Co., St. Louis, Mo.), 0.2 mg/ml of 0.5 M citrate-phosphate buffer, pH 4.5. Hex activity was visualized as fluorescence under long wave UV light. Column Chromatography. Ion exchange chroma-. tography of Hex was carried out as described by Beutler et al. (8), except that the maximal NaCI concentration in the gradient was increased from 0.4 to 0.6 M.Immunological Studies. Rabbit antisera against human Hex

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Insulin-like growth factor I receptor primary structure: comparison with insulin receptor suggests structural determinants that define functional specificity.

Ullrich¹,

Gray²,

Tam³

et al. 1986

The EMBO Journal

1,748

883

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To identify structural characteristics of the closely related cell surface receptors for insulin and IGF‐I that define their distinct physiological roles, we determined the complete primary structure of the human IGF‐I receptor from cloned cDNA. The deduced sequence predicts a 1367 amino acid receptor precursor, including a 30‐residue signal peptide, which is removed during translocation of the nascent polypeptide chain. The 1337 residue, unmodified proreceptor polypeptide has a predicted Mr of 151,869, which compares with the 180,000 Mr IGF‐I receptor precursor. In analogy with the 152,784 Mr insulin receptor precursor, cleavage of the Arg‐Lys‐Arg‐Arg sequence at position 707 of the IGF‐I receptor precursor will generate alpha (80,423 Mr) and beta (70,866 Mr) subunits, which compare with approximately 135,000 Mr (alpha) and 90,000 Mr (beta) fully glycosylated subunits.

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Confirmation of the synteny of the human genes for mannose phosphate isomerase and pyruvate kinase and of their assignment to chromosome 15

Cited by 16 publications

References 7 publications

Chromosomal localization of the gene for a human cytosolic thyroid hormone binding protein homologous to the subunit of pyruvate kinase, subtype M2

Chromosomal localization of the gene for a human cytosolic thyroid hormone binding protein homologous to the subunit of pyruvate kinase, subtype M2

Characterization of heteropolymeric hexosaminidase A in human X mouse hybrid cells.

Insulin-like growth factor I receptor primary structure: comparison with insulin receptor suggests structural determinants that define functional specificity.

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