Confirmation of the transmission of barley yellow mosaic virus (BaYMV) by the fungus <i>Polymyxa graminis</i>

Adams, Michael J.; Swaby, A. G.; Jones, Philip W.

doi:10.1111/j.1744-7348.1988.tb02048.x

Cited by 72 publications

(28 citation statements)

References 11 publications

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“…This suggests that the 73 kDa protein may be important for enabling the transmission of BaMMV by its natural vector. This hypothesis is supported by the findings of Adams et al (1988) who have demonstrated that repeated mechanical transmission of the Streatley isolate of barley yellow mosaic virus, which has now been classified as BaMMV (Andersen et al~ 1993), resulted in a strain that could not be acquired and/or transmitted by the vector. It seems reasonable to speculate that the 73 kDa protein possesses at least one additional function because all the deletions known so far are restricted to the C-terminal half of the protein.…”

Section: P2t>supporting

confidence: 73%

In vitro transcripts of a full-length cDNA of a naturally deleted RNA2 of barley mild mosaic virus (BaMMV) replicate in BaMMV-infected plants

Timpe

Kühne

1995

Journal of General Virology

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The RNA2 of a German isolate of the bipartite barley mild mosaic bymovirus (BaMMV-ASL 1) is 3524 nucleotides long excluding the 3'-terminal poly(A) tail. The isolate was propagated by mechanical inoculation for several years. Electrophoretic comparison of viral nucleic acids during this period revealed that a spontaneous reduction in the length of the RNA2 occurred, resulting in the isolate BaMMV-ASLla. The deleted RNA2 of BaMMV lacked a fragment that was 630 nucleotides long. The deletion occurred in the 3' half of the single open reading flame (ORF) found in RNA2, this ORF encodes a polyprotein that has a molecular mass of 98 kDa, which is assumed to be processed autocatalytically into proteins of 25 kDa and 73 kDa. A full-length eDNA of the deleted RNA2 was synthesized and cloned under the control of the phage T7 promoter. In vitro transcripts of the BaMMV-ASLIa clone replicated in barley plants after co-inoculation with a wildtype-like isolate of BaMMV. The deletions in RNA2 of BaMMV-ASLla and those of a number of other isolates that were examined were found to affect a domain of the putative 73 kDa protein that is obviously not essential for replication but may be important for the transmission of BaMMV by its natural vector Polymyxa graminis.

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Section: P2t>supporting

confidence: 73%

In vitro transcripts of a full-length cDNA of a naturally deleted RNA2 of barley mild mosaic virus (BaMMV) replicate in BaMMV-infected plants

Timpe

Kühne

1995

Journal of General Virology

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“…Barley mild mosaic virus (BaMMV) (Rao and Brakke 1969;Adams et al 1988). Virus acquisition occurs as the fungus develops within a virus-infected host cell, even within the first vegetative generation of the vector (Campbell and Grogan 1964).…”

Section: P Graminis-soilborne Wheat Mosaic Virus (Sbwmv) Andmentioning

confidence: 99%

The Fungal Transmitted Viruses

2008

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“…Barley yellow mosaic virus (BaYMV-1, BaYMV-2) and barley mild mosaic virus (BaMMV) (Adams et al, 1988) from where they spread systemically throughout the plant. Due to the soil-borne transmission of the viruses, barley plants cannot be protected by chemical means and cultivation of winter barley in infested areas depends entirely on the availability of disease-resistant cultivars.…”

Section: Introductionmentioning

confidence: 99%