Confirmatory Detection of <i>Escherichia coli</i> O157: H7 in Diarrhoeic and Non-Diarrhoeic Calves by using real time PCR with Studying the Antimicrobial Susceptibility of these bacteria

Hasan, Mahboob; Hussein, Mohammed Ali; Yousif, Afaf Abdulrahman

doi:10.5958/0974-360x.2019.00046.5

Cited by 6 publications

(5 citation statements)

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“…A study of Ojo et al (2014) appeared that the E. coli O157:H7 isolates found in 16.1% and 26.9% of the, fecal samples collected from dogs with and without diarrhea (7). In Iraq, Hasan et al, (8) revealed that E. coli O157:H7 isolates found in the 32 samples from 350 samples which collected from calves and dogs, (32 samples were positive for the pathogen including 4 isolates from calves with diarrhea and 28 isolates from calves without diarrhea), and included rfbO157 and flics H7 genes (8). In additional study, 32 isolates were detected in calves with and without diarrhea in Fallujah city, and contained Stx1, Stx2 and eae genes (9).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of rfbO157, shiga toxins and hemolysin genes for Escherichia coli O157: H7 from canine feces in Tikrit and Mosul cities, Iraq

Abdulrazzaq¹,

Owain²,

Majeed³

et al. 2021

IJVS

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Escherichia coli O157:H7 is considered as an important pathogen of diarrhea in adult dogs and puppies because it contains virulence genes. The study objective was to the molecular detection of the rfbO157 encoding the O-antigen specific for E. coli O157: H7,shiga toxins and hemolysin genes of E.coli O157:H7 in feces of dogs that collected from different regions in Tikrit and Mosul cities, Iraq. One hundred fecal swabs were collected from pet and K9 dogs including (72 dogs with diarrhea, and 28 without diarrhea). All the Collected swabs were cultured in the nutrient and MacConkey agars, Then the suspected colonies were cultured in the EMB agar. Metallic sheen colonies were cultured by using the chrome agar. All bacteriological identified isolates were enrolled by using the polymerase chain reaction (PCR) technique. The results of this study showed that 7(9.7%) of 72 dogs suffered from diarrhea were positive for E. coli O157:H7 that contained the rfbO157 gene (n= 6), carry stx1 gene (n= 3), carry stx2 gene (n= 3), and hlyA gene (n= 1). On the other hand, 2 (7.1%) of 28 dogs without diarrhea were positive for E. coli O157:H7 that contained the rfbO157 gene (n= 1), stx2 gene (n= 1), and hlyA gene (n= 1). In conclusion, dogs can be a significant reservoir for pathogenic E. coli O157:H7, particularly dogs with diarrhea.

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Section: Introductionmentioning

confidence: 99%

Molecular detection of rfbO157, shiga toxins and hemolysin genes for Escherichia coli O157: H7 from canine feces in Tikrit and Mosul cities, Iraq

Abdulrazzaq¹,

Owain²,

Majeed³

et al. 2021

IJVS

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“…The antibiotic resistance profile revealed that 60% were ESC resistant E. coli, while the remaining strains (40%) were ESC susceptible E. coli but resist to other non β-lactams. Variations in antibiotic resistance within E. coli O157 strains were detected by Mora et al (2005), Srinivasan et al (2007) and Hasan et al (2019). The difference in resistance patterns may be attributable to the most prevalent types of veterinary antibiotics used in different geographical regions for treatment and prevention (Saei et al 2012;El Jalil et al 2020;Abd El-Fatah et al 2020).…”

Section: Figmentioning

confidence: 98%

Volume 10, No. 1, 2021

2021

Int J Vet Sci

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E. coli O157 is one of the main food-borne pathogens that attributed to the attaching and effacing Shigatoxigenic E. coli pathotype (AE-STEC). The occurrence of E. coli O157 in diarrheic calves investigated through molecular detection of rfbEO157 encoding gene was 8.02% within E. coli strains. Detection of AE-STEC virulence genes in E. coli O157 strains using multiplex PCR showed the presence of eae, stx1, stx2 and ehylA in percentages of 93.3, 73.3, 20 and 13.3%, respectively. The virulence genes profile of E. coli O157 revealed the predominance of eae+stx1 combination in 66.7% of these strains. All E. coli O157 strains exhibited antibiotic multi-resistances with higher resistance (100%) to amoxicillin/clavulanic acid, cefalexin, cefuroxime and tetracycline, while the lowest resistance was detected for gentamicin (40%). Phenotypic resistance to extended spectrum cephalosporins (ESCs) indicated that 60% of these strains were resistant to ceftriaxone and cefotaxime, while 53.3% were resistant to cefquinome. Molecular detection of extended spectrum β-lactamases (ESBLs) encoding genes recorded the superiority of blaTEM gene (100%), whereas the blaSHV and blaCTXM genes were detected in percentages of 40 and 20%, respectively. The genetic profiling of resistance genes revealed the role of blaCTXM gene in ESCs resistance as blaTEM+blaSHV+blaCTXM combination was detected only in ESC resistant strains. Finally, B2 phylogroup was the most prevalent one (80%) within E. coli O157 strains. This implicates diarrheic calves as a source of highly pathogenic multi-resistant E. coli O157 strains.

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“…Shiga-toxigenic E. coli O157: H7 -this is a type of Shiga toxin E. coli, which can cause diseases in humans and animals. In studies [11], the sensitivity test of isolates showed that all isolated cultures are highly resistant to erythromycin (100%) and cephalotin (96.87%). Less resistance to tetracycline, trimethoprim-sulfamethoxazole and chloramphenicol at different percentages (31.25%), (12.5%) and (9.37%), respectively, and all isolates showed high sensitivity to gentamicin (100%), enrofloxacin (100%).…”

Section: Introductionmentioning

confidence: 99%

Silver-based drug effect on the body of calves with diarrhea

et al. 2020

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Diarrhea of newborn calves is a globally common disease that causes significant damage to livestock. Economic losses from diarrhea in young cattle are directly related to the cost of long-term treatment of animals and their mortality, as well as the projected decrease in productivity, which negatively affects the efficiency of maintaining the entire herd. Currently, it is an established fact that diarrhea of newborn calves has a polyethological nature, which has an interaction in pathogenesis between the calf’s body, enteropathogens and the technology of keeping animals. For the treatment of calves with diarrhea caused by enteropathogenic Escherichia coli, many methods and schemes using various antibiotics have been proposed. The need for fast and efficient treatment for acute diarrhea of calves in many cases leads to an empirical selection of the drug without taking into account the spectrum of its activity. This approach leads to the appearance and rapid spread of antibiotic resistant strains of microorganisms and as a result reduces the effectiveness of veterinary measures. The article presents the results of a study of the effect of a new complex drug containing stabilized silver particles, an immunostimulator and an antioxidant on the body of calves with diarrhea, as an alternative to the antibiotics. Studies were conducted on calves of the first week of life with a diagnosis of diarrhea, with enteropathogenic Escherichia coli confirmation.

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Confirmatory Detection of Escherichia coli O157: H7 in Diarrhoeic and Non-Diarrhoeic Calves by using real time PCR with Studying the Antimicrobial Susceptibility of these bacteria

Cited by 6 publications

References 0 publications

Molecular detection of rfbO157, shiga toxins and hemolysin genes for Escherichia coli O157: H7 from canine feces in Tikrit and Mosul cities, Iraq

Molecular detection of rfbO157, shiga toxins and hemolysin genes for Escherichia coli O157: H7 from canine feces in Tikrit and Mosul cities, Iraq

Volume 10, No. 1, 2021

Silver-based drug effect on the body of calves with diarrhea

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