Confocal imaging of autonomic preganglionic neurons in the spinal cord of the caecilian Typhlonectes natans (Amphibia: Gymnophiona)

Zaccone, Daniele; Lauriano, Eugenia Rita; Capillo, Gioele; Żuwała, Krystyna; Budzik, Karolina A.; Kuciel, Michał; Zaccone, Giacomo

doi:10.1016/j.acthis.2014.09.004

Cited by 6 publications

(7 citation statements)

References 23 publications

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“…Techniques for immunolabeling were similar to those previously described for a wide variety of tissues from fish visceral organs and skin [20][21][22][23][24][25][26][27]. Double immunostainings were carried out at room temperature.…”

Section: Immunohistochemistry and Confocal Immunofluorescence Microscopymentioning

confidence: 99%

Expression of acetylcholine, its contribution to regulation of immune function and O2 sensing and phylogenetic interpretations of the African butterfly fish Pantodon buchholzi (Osteoglossiformes, Pantodontidae)

Capillo

Zaccone

Cupello

et al. 2021

Fish & Shellfish Immunology

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Section: Immunohistochemistry and Confocal Immunofluorescence Microscopymentioning

confidence: 99%

Expression of acetylcholine, its contribution to regulation of immune function and O2 sensing and phylogenetic interpretations of the African butterfly fish Pantodon buchholzi (Osteoglossiformes, Pantodontidae)

Capillo

Zaccone

Cupello

et al. 2021

Fish & Shellfish Immunology

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“…Techniques for immunolabeling were similar to those previously described for a wide variety of fish tissues (Zaccone et al, 2014. Double immunostainings were carried out at room temperature.…”

Section: Immunohistochemistry Confocal Immunofluorescence Microscopymentioning

confidence: 99%

Identification and distribution of neuronal nitric oxide synthase and neurochemical markers in the neuroepithelial cells of the gill and the skin in the giant mudskipper, Periophthalmodon schlosseri

Zaccone

Lauriano

Kuciel

et al. 2017

Zoology

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“…; Zaccone et al . ). ChAT antibodies were monoclonals produced in mouse (Product number WH0001103M1‐100UG; Sigma‐Aldrich).…”

Section: Methodsmentioning

confidence: 97%

“…; Zaccone et al . ). Pre‐absorption of these primary antisera according to the guidelines of the suppliers led to complete elimination of the immunostaining in all cases.…”

Section: Methodsmentioning

confidence: 97%

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Polymorphous granular cells in the lung of the primitive fish, the bichir Polypterus senegalus

et al. 2015

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Zaccone, D., Icardo, J. M., Kuciel, M., Alesci, A., Pergolizzi, S., Satora, L., Lauriano, E. R., Zaccone, G. 2015. Polymorphous granular cells in the lung of the primitive fish, the bichir Polypterus senegalus. -Acta Zoologica (Stockholm) 00: 000-000.This study describes the distribution and the coexpression of specific neurochemical markers in both neuroendocrine-like cells (NEC-like) and polymorphous granular cells (PGCs) that populate the mucociliated epithelium of the lung in the air-breathing fish Polypterus senegalus, using confocal immunohistochemistry. Using confocal immunohistochemistry, we determined the coexpression of specific neurochemical markers. Colocalization studies showed that 5HT is coexpressed with calbindin and nNOS and choline acetyltransferase (ChAT) is coexpressed with nNOS in the PGCs. This study also shows for the first time the simultaneous occurrence of piscidin 1 and 5HT in the PGCs. The function of these cells being equivalent to ones found in fish gill subepithelial parenchyma is still not known. Due to the importance of piscidin 1 in local immune defence, more research is to be useful to understand a possible interaction of PGCs with immune response in the bichir lung. In fact, the capacity of PGCs to produce NO and other neuroactive substances found in immune cells of fish may represent a primitive form of immunoregulation of innate immunity and specifically antimicrobial function as NO induction and respiratory burst activity are correlated with immune response.

show abstract

Confocal imaging of autonomic preganglionic neurons in the spinal cord of the caecilian Typhlonectes natans (Amphibia: Gymnophiona)

Cited by 6 publications

References 23 publications

Expression of acetylcholine, its contribution to regulation of immune function and O2 sensing and phylogenetic interpretations of the African butterfly fish Pantodon buchholzi (Osteoglossiformes, Pantodontidae)

Expression of acetylcholine, its contribution to regulation of immune function and O2 sensing and phylogenetic interpretations of the African butterfly fish Pantodon buchholzi (Osteoglossiformes, Pantodontidae)

Identification and distribution of neuronal nitric oxide synthase and neurochemical markers in the neuroepithelial cells of the gill and the skin in the giant mudskipper, Periophthalmodon schlosseri

Polymorphous granular cells in the lung of the primitive fish, the bichir Polypterus senegalus

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