Confocal imaging of protein distributions in porous silicon optical structures

Stefano, Luca De; D’Auria, Sabato

doi:10.1088/0953-8984/19/39/395009

Cited by 12 publications

(5 citation statements)

References 15 publications

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“…Figure 8D quantifies the intensity profiles of the average fluorescence signal and it could be clearly seen that the labeled protein was distributed as a Gaussian function having its maximum value close to the center of the layer. This result further confirm that the protein was penetrated inside the pores (De Stefano and D'Auria, 2007 ).…”

Section: Resultssupporting

confidence: 81%

Toward Multi-Parametric Porous Silicon Transducers Based on Covalent Grafting of Graphene Oxide for Biosensing Applications

Moretta¹,

Terracciano

Dardano

et al. 2018

Front. Chem.

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Graphene oxide (GO) is a two-dimensional material with peculiar photoluminescence emission and good dispersion in water, that make it an useful platform for the development of label-free optical biosensors. In this study, a GO-porous silicon (PSi) hybrid device is realized using a covalent chemical approach in order to obtain a stable support for biosensing applications. Protein A, used as bioprobe for biosensing purposes, is covalently linked to the GO, using the functional groups on its surface, by carbodiimide chemistry. Protein A bioconjugation to GO-PSi hybrid device is investigated by atomic force microscopy (AFM), scanning electron microscopy (SEM), water contact angle (WCA) measurements, Fourier transform infrared (FTIR) spectroscopy, steady-state photoluminescence (PL), and fluorescence confocal microscopy. PSi reflectance and GO photoluminescence changes can thus be simultaneously exploited for monitoring biomolecule interactions as in a multi-parametric hybrid biosensing device.

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Section: Resultssupporting

confidence: 81%

Toward Multi-Parametric Porous Silicon Transducers Based on Covalent Grafting of Graphene Oxide for Biosensing Applications

Moretta¹,

Terracciano

Dardano

et al. 2018

Front. Chem.

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“…The etching depth and accordingly the pore length can be simply controlled via the etching time [25,61]. Moreover, on demand, the porous matrix can be detached from the underlying silicon wafer after finishing the etching process rendering this porous structure particularly versatile for studies of nanopore-condensed matter [4,29,[62][63][64][65][66][67][68][69][70][71][72][73][74][75].…”

Section: Film Adsorption and Capillary Condensationmentioning

confidence: 99%

Soft matter in hard confinement: phase transition thermodynamics, structure, texture, diffusion and flow in nanoporous media

Huber

2015

J. Phys.: Condens. Matter

250

287

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Spatial confinement in nanoporous media affects the structure, thermodynamics and mobility of molecular soft matter often markedly. This article reviews thermodynamic equilibrium phenomena, such as physisorption, capillary condensation, crystallisation, self-diffusion, and structural phase transitions as well as selected aspects of the emerging field of spatially confined, non-equilibrium physics, i.e. the rheology of liquids, capillarity-driven flow phenomena, and imbibition front broadening in nanoporous materials. The observations in the nanoscale systems are related to the corresponding bulk phenomenologies. The complexity of the confined molecular species is varied from simple building blocks, like noble gas atoms, normal alkanes and alcohols to liquid crystals, polymers, ionic liquids, proteins and water. Mostly, experiments with mesoporous solids of alumina, gold, carbon, silica, and silicon with pore diameters ranging from a few up to 50 nm are presented. The observed peculiarities of nanopore-confined condensed matter are also discussed with regard to applications. A particular emphasis is put on texture formation upon crystallisation in nanoporous media, a topic both of high fundamental interest and of increasing nanotechnological importance, e.g. for the synthesis of organic/inorganic hybrid materials by melt infiltration, the usage of nanoporous solids in crystal nucleation or in template-assisted electrochemical deposition of nano structures.

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“…In this work, we evaluate mesoporous silicon microparticles as a nontoxic − delivery system for the more effective delivery of anthelmintic protein Cry5B . Cry5B toxin is a large (140 kDa) protein composed of three distinct domains, with the overall dimensions of 85 × 65 × 45 Å 3 . , The pore dimensions in electrochemically generated mesoporous Si can be adjusted to a size adequate to admit this protein ,, but sufficiently small to inhibit the action of proteolytic enzymes. − For this study, we investigated a partially oxidized form of pSi because the oxide surface has been shown to be compatible with sensitive proteins, ,− and the negatively charged surface allows concentration of positively charged proteins in the pores via electrostatic forces. , Furthermore, oxidized pSi has been shown to be stable under acidic conditions but readily dissolves at pH >7, which is advantageous for an oral route of delivery to the intestines. , …”

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confidence: 99%

Protection and Delivery of Anthelmintic Protein Cry5B to Nematodes Using Mesoporous Silicon Particles

Miller

et al. 2015

ACS Nano

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The ability of nano- and microparticles of partially oxidized mesoporous silicon (pSi) to sequester, protect, and deliver the anthelmintic pore-forming protein Cry5B to nematodes is assessed in vitro and in vivo. Thermally oxidized pSi particles are stable under gastric conditions and show relatively low toxicity to nematodes. Fluorescence images of rhodamine-labeled pSi particles within the nematodes Caenorhabditis elegans and Ancylostoma ceylanicum show that ingestion is dependent on particle size: particles of a 0.4 ± 0.2 μm size are noticeably ingested by both species within 2 h of introduction in vitro, whereas 5 ±2 μm particles are excluded from C. elegans but enter the pharynx region of A. ceylanicum after 24 h. The anthelmintic protein Cry5B, a pore-forming crystal (Cry) protein derived from Bacillus thuringiensis, is incorporated into the pSi particles by aqueous infiltration. Feeding of Cry5B-loaded pSi particles to C. elegans leads to significant intoxication of the nematode. Protein-loaded particles of size 0.4 μm display the highest level of in vitro toxicity toward C. elegans on a drug-mass basis. The porous nanostructure protects Cry5B from hydrolytic and enzymatic (pepsin) degradation in simulated gastric fluid (pH 1.2) for time periods up to 2 h. In vivo experiments with hookworm-infected hamsters show no significant reduction in worm burden with the Cry5B-loaded particles, which is attributed to slow release of the protein from the particles and/or short residence time of the particles in the duodenum of the animal.

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Confocal imaging of protein distributions in porous silicon optical structures

Cited by 12 publications

References 15 publications

Toward Multi-Parametric Porous Silicon Transducers Based on Covalent Grafting of Graphene Oxide for Biosensing Applications

Toward Multi-Parametric Porous Silicon Transducers Based on Covalent Grafting of Graphene Oxide for Biosensing Applications

Soft matter in hard confinement: phase transition thermodynamics, structure, texture, diffusion and flow in nanoporous media

Protection and Delivery of Anthelmintic Protein Cry5B to Nematodes Using Mesoporous Silicon Particles

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