Confocal laser endomicroscopy for in vivo diagnosis of gastric intraepithelial neoplasia: a feasibility study

Flexible spectral imaging color enhancement plus probe‐based confocal laser endomicroscopy for gastric intestinal metaplasia detection

“…This combined technique provided comparable sensitivity (96%), specificity (90%), PPV (86%), NPV (97%), and accuracy (92%) to the previous eCLE study . The technique is practical because the mean duration of each procedure was approximately 29 min and required no more time than the previous studies that used standard random biopsy …”

Dipeptidase 1 (DPEP1) is a marker for the transition from low-grade to high-grade intraepithelial neoplasia and an adverse prognostic factor in colorectal cancer

“…In the present study, we have identified DPEP1 as a highly specific marker of high-grade IEN and CRCs. On-going improvements in the field of gastrointestinal endoscopic surveillance including real-time histology, endocytoscopy, chromoscopy and confocal laser endomicroscopy (Kiesslich et al , 2007; Li et al , 2010) may in the future provide a routine diagnostic platform to screen for DPEP1 in vivo as a predictive marker for high-grade IEN. Using DPEP1's enzymatic activity or its shed CRD for visualising high-grade IEN as potential diagnostic determinants in vivo may improve the efficacy of endoscopic surveillance.…”

Confocal Laser Endomicroscopy and Molecular Imaging in Barrett Esophagus and Stomach

“…A prospective, double-blind, feasibility study on the use of eCLE with fluorescein as the fluorescent agent showed low diagnostic accuracy (79.8%) for gastric intraepithelial neoplasia and low sensitivity (66.7%) in distinguishing between low- and high-grade intraepithelial neoplasia 30. This result can be explained based on the inability of CLE using fluorescein to accurately assess the nuclear-cytoplasmic ratio, nuclear pleomorphism, and hyperchromatism, which are important pathologic features for grading the degree of neoplasia.…”