Confocal Microscopy Improves 3D Microdosimetry Applied to Nanoporation Experiments Targeting Endoplasmic Reticulum

Angelis, Annalisa De; Denzi, Agnese; Merla, Caterina; Andre, Frank M; Mir, Lluis M.; Apollonio, Francesca; Liberti, Micaela

doi:10.3389/fbioe.2020.552261

Cited by 18 publications

(18 citation statements)

References 32 publications

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“…To obtain the 3D realistic model of MSC, with both ER and nuclear compartments, a set of images at different z-quotes was acquired using a confocal microscope and specific fluorescent dye to decorate ER and nuclear compartments as described in [22]. Each image was processed with a custom automated routine computed in MATLAB software, able to convert the RGB images into a 3D STL file, as detailed in [15]. This 3D STL file has been directly imported in Comsol Multiphysics software v. 5.3 to perform the simulation.…”

Section: Modelling a 3d Realistic Cell A Microdosimetric Investigatio...mentioning

confidence: 99%

“…The latter is currently used to build up reliable geometric models able to follow the shape of cellular and sub-cellular structures, thus affecting the local EM quantities. Together with the shape and spatial orientation of the cell compartments, dielectric properties play an important role on the EMFs exposure as recently demonstrated for different types of cells and intracellular compartments such as 2D neurons [9], 2D neuroblastoma cells [10], 2D hamster fibroblasts [11], 3D keratinocyte [12,13], 3D erythrocyte [14] and 3D endoplasmic reticulum [15].…”

Section: Introductionmentioning

confidence: 95%

“…In this work, we present a 3D realistic model of a human mesenchymal stem cell (MSC), including irregular details of the endoplasmic reticulum (ER) and the nucleus. The geometrical model is derived from the confocal microscopy images of MSC cells decorated to show both the ER and the nucleus compartments, as previously described in [15].…”

Section: Introductionmentioning

confidence: 99%

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A microdosimetric study at the cellular and intracellular level using a 3D realistic cell model

Caramazza

Angelis

Haider

et al. 2022

2021 51st European Microwave Conference (EuMC)

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Section: Modelling a 3d Realistic Cell A Microdosimetric Investigatio...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

See 1 more Smart Citation

A microdosimetric study at the cellular and intracellular level using a 3D realistic cell model

Caramazza

Angelis

Haider

et al. 2022

2021 51st European Microwave Conference (EuMC)

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“…Lastly, E distribution was computed from the induced electric potential distribution in the cell and organelles (E = −∇V ). The numerical method used in this work was previously validated in various studies [18]- [21], [24]- [26].…”

Section: Numerical Modelmentioning

confidence: 99%

“…The MTNM and DNM employ equivalent distributed circuits to represent spatial variation of various cellular compartments. Moreover, FEM has been extensively employed to compute the transmembrane potential (TMP) of 2D neurons [18], three dimensional (3D) Chinese hamster ovary cell [19], 3D erythrocyte [20], super formula based 3D keratinocytes [21], 2D nucleus [22], 2D [23], and 3D [24] endoplasmic reticulum (ER) and axisymmetric model of isolated mitochondria [25]. Additionally, the numerical investigations performed in [18] and [20] employed dispersive electromagnetic cell model.…”

Section: Introductionmentioning

confidence: 99%

Local Dosimetry at Cellular and Subcellular Level in HF and Millimeter-Wave Bands

Haider¹,

Nikolayev²,

Dréan³

et al. 2021

IEEE J. Microw.

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The aim of this study was to investigate quantitatively local sub-cellular power deposition at frequencies upcoming for wireless power transfer (WPT) and millimeter-wave (mmWave) technologies. The study was performed on a realistic two-dimensional keratinocyte cell model, designed based on electron microscopy images and experimental data on surface area fraction of keratinocyte to explicitly represent nucleus, mitochondria, endoplasmic reticulum, Golgi apparatus and vesicles. The average power loss density (PLD avg ) and electric field (E avg ) were computed by solving Laplace's equation under quasi-static approximation using the finite element method. The numerical results for the spherical cell model were validated with corresponding analytical solutions. The results showed that E avg and PLD avg inside the organelles increased with frequency. Nearly, 51.8% and 98.9% of the incident field on the cell penetrated inside the organelles at 6.78 MHz and 60 GHz, respectively. The PLD avg within the organelles in average was 35.7% (6.78 MHz) and 1.95% (60 GHz) lower than in the cytoplasm. The E avg induced inside nuclear pores (N p ) exceeded the incident field by 5 times and 1.1 times at 6.78 MHz and 60 GHz, respectively. The corresponding PLD avg within N p was 32.7 times (6.78 MHz) and 1.2 times (60 GHz) higher than that of the cytoplasm. The enhancement of PLD avg in N p suggests that the intracellular traffic is locally exposed to higher exposure levels compared to the background PLD avg in cytosol.INDEX TERMS Dosimetry, finite element method, human skin, millimeter waves.This article has been accepted for inclusion in a future issue of this journal. Content is final as presented, with the exception of pagination.

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The Importance of Subcellular Structures to the Modeling of Biological Cells in the Context of Computational Bioelectromagnetics Simulations

Jerbic

Svejda

Sievert

et al. 2023

Bioelectromagnetics

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Numerical investigation of the interaction of electromagnetic fields with eukaryotic cells requires specifically adapted computer models. Virtual microdosimetry, used to investigate exposure, requires volumetric cell models, which are numerically challenging. For this reason, a method is presented here to determine the current and volumetric loss densities occurring in single cells and their distinct compartments in a spatially accurate manner as a first step toward multicellular models within the microstructure of tissue layers. To achieve this, 3D models of the electromagnetic exposure of generic eukaryotic cells of different shape (i.e. spherical and ellipsoidal) and internal complexity (i.e. different organelles) are performed in a virtual, finite element method-based capacitor experiment in the frequency range from 10 Hz to 100 GHz. In this context, the spectral response of the current and loss distribution within the cell compartments is investigated and any effects that occur are attributed either to the dispersive material properties of these compartments or to the geometric characteristics of the cell model investigated in each case. In these investigations, the cell is represented as an anisotropic body with an internal distributed membrane system of low conductivity that mimics the endoplasmic reticulum in a simplified manner. This will be used to determine which details of the cell interior need to be modeled, how the electric field and the current density will be distributed in this region, and where the electromagnetic energy is absorbed in the microstructure regarding electromagnetic microdosimetry. Results show that for 5 G frequencies, membranes make a significant contribution to the absorption losses. Bioelectromagnetics. 44:26-46, 2023.

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Confocal Microscopy Improves 3D Microdosimetry Applied to Nanoporation Experiments Targeting Endoplasmic Reticulum

Cited by 18 publications

References 32 publications

A microdosimetric study at the cellular and intracellular level using a 3D realistic cell model

A microdosimetric study at the cellular and intracellular level using a 3D realistic cell model

Local Dosimetry at Cellular and Subcellular Level in HF and Millimeter-Wave Bands

The Importance of Subcellular Structures to the Modeling of Biological Cells in the Context of Computational Bioelectromagnetics Simulations

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