Conformation, activity and proteolytic stability of acid phosphatase on clay minerals and soil colloids from an Alfisol

Huang, Qiaoyun; Zhu, Jun; Qiao, Xueqing; Cai, Peng; Rong, Xingmin; Liang, Wei; Chen, Wenli

doi:10.1016/j.colsurfb.2009.07.031

Cited by 21 publications

(9 citation statements)

References 23 publications

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“…Decoupled V max and K m pH optima have been observed for purified enzymes in nonsoil contexts, including calf intestinal alkaline phosphatase (Fernley & Walker, 1965), bovine milk acid phosphatase (Andrews & Pallavicini, 1973), and chymotrypsin (Renard & Fersht, 1973). Apparent kinetic properties of soil enzymes are likely to exhibit more complex relationships with pH than purified enzymes characterized in vitro because in soils multiple isozymes occur and with heterogeneously distribution across and within soil solid and liquid phases (George et al., 2007; Huang et al., 2009; Quiquampoix, 1987, 2000; Quiquampoix & Burns, 2007).…”

Section: Resultsmentioning

confidence: 99%

Apparent kinetic properties of soil phosphomonoesterase and β‐glucosidase are disparately influenced by pH

Margenot

2021

Soil Science Soc of Amer J

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Extracellular enzymes catalyze organic matter transformations in soils, yet the study of soil enzymes does not typically evaluate how enzyme kinetic properties that govern activity in situ may shift with environmental parameters such as pH. To test this, we quantified changes in apparent kinetic properties (maximum catalysis rate [AppVmax] and substrate concentration that produces 50% of Vmax [AppKm]) of soil phosphomonoesterase (PME) and β‐glucosidase (BG) as a function of pH by using dual gradients of buffer pH (3–12) and substrate concentrations in a selected set of diverse soils. A third kinetic property, apparent specificity constant (AppKa = AppVmax/AppKm) was also evaluated as a function of pH. Results indicated widely differing pH optima of soil PME and BG AppVmax and AppKm, decoupling of which entailed unpredictable pH optima of AppKa. Additionally, changes in soil enzyme activity with pH may not be driven by shifts in the specificity constant because pH–AppVmax relationships are largely unrelated to co‐occurring changes in AppKa. These results invalidate the assumption that Km of a soil enzyme is constant across pH and emphasize the importance of assaying soil enzyme activities at sufficiently high (5 × Km) substrate concentrations at a given pH to avoid the mischaracterizing pH optima.

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Section: Resultsmentioning

confidence: 99%

Apparent kinetic properties of soil phosphomonoesterase and β‐glucosidase are disparately influenced by pH

Margenot

2021

Soil Science Soc of Amer J

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“…It is important to consider a soil’s granulometry when soil enzymes are studied, because they are often immobilized onto these particles. Several authors reported that enzyme immobilization on soil particles provides stability to this biocatalyst against unfavorable soil environment. , Then, the smaller the particle size, the higher the area available for enzyme immobilization. Indeed, considering that Cerrado soils present a high amount of clay, it is possible to assume that these soils constitute a favorable environment for soil enzymes.…”

Section: Discussionmentioning

confidence: 99%

Effects of Organic and Conventional Management of Sugar Cane Crop on Soil Physicochemical Characteristics and Phosphomonoesterase Activity

Purcena

Leal

Leandro

et al. 2014

J. Agric. Food Chem.

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Soil enzymes play an important role in agriculture and particularly in nutrient cycling. They are also involved in the degradation, transformation, and mineralization of organic matter and availability of nutrients in soil. It is believed that organic agriculture causes fewer losses to soil quality and is less aggressive to the environment than conventional management. In this study, the effects of conventional (CM) and organic management (OM) on phosphomonoesterases, an important enzyme for soil fertility, were evaluated and compared to those results from native Cerrado (Brazilian Savanna) soil (NS), because they are the most common phosphatases in soils. The results showed that there were both acid (AcP) and alkaline (AkP) phosphatases in all soils tested and that AcP activity was higher than that of AkP. In contrast to AkP, AcP had its activity affected by land use. In the cultivated areas there was a reduction of almost 50% of AcP activity respect to native unexploited soils and there was no significant difference between organic and CM, demonstrating that independent of the management chosen, there was an impact of land use on AcP activity. Principal component analysis indicated that characteristics related to pH such as alkali saturation (V%), aluminum saturation (M%), Al(3+), soil total acidity (H+Al), and Ca(2+) are the main factors that permit distinguishing NS from OM and CM.

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“…In addition to providing substrates, organic colloids protect these enzymes (AR, β-G, AP, BP and FDA) from degradation by proteolytic enzymes, contributing to the maintenance of their catalytic capacity (Huang et al, 2009;Li et al, 2018).…”

Section: /9mentioning

confidence: 99%

Soil enzymatic activity and chemical attributes after continuous and interrupted application of pig slurry

Navroski¹,

Filho²,

Barbosa³

et al. 2019

Agraria

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The objective of this work was to evaluate enzymatic activities and chemical attributes of a soil under continuous application for 28 years and after interruption for 5 and 12 months of pig slurry (PS). The soil received doses of 0, 30 and 60 m 3 ha-1 year-1 of PS since 1988, and in 2016, the plots were divided in half and there was interruption of the application of PS in half plot, generating a 2 × 2 factorial scheme, being two doses of PS (30 and 60 m 3 ha-1 year-1) and two application conditions (continuous or interrupted). The available P, total organic carbon (TOC), pH, Al 3+ , H + + Al 3+ , Ca 2+ , Mg 2+ and K + and activity of the enzymes arylsulfatase (AR), β-glycosidase (β-G), acid (AP) and basic (BP) phosphatase , hydrolysis of fluorescein diacetate (FDA) of soil were assessed. The content of P, Mg 2+ and H + + Al 3+ varied with the doses of PS applied, but not with the interruption of the applications. The continuous application of PS increased the activity of the enzymes AR, β-G and BP, whereas FDA and AP were not influenced by the interruption of the applications. The interruption of PS application for up to one year did not change the chemical attributes of the soil, but reduces the activity of the enzymes AR, β-G and BP.

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Conformation, activity and proteolytic stability of acid phosphatase on clay minerals and soil colloids from an Alfisol

Cited by 21 publications

References 23 publications

Apparent kinetic properties of soil phosphomonoesterase and β‐glucosidase are disparately influenced by pH

Apparent kinetic properties of soil phosphomonoesterase and β‐glucosidase are disparately influenced by pH

Effects of Organic and Conventional Management of Sugar Cane Crop on Soil Physicochemical Characteristics and Phosphomonoesterase Activity

Soil enzymatic activity and chemical attributes after continuous and interrupted application of pig slurry

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