Conformation and activity of lysozyme on binding to two types of gold nanorods: A comparative study

Moghadam, Tahereh Tohidi; Ranjbar, Bijan; Khajeh, Khosro

doi:10.1016/j.ijbiomac.2012.04.020

Cited by 22 publications

(8 citation statements)

References 37 publications

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“…Gold Nanorods were synthesized according to seed mediated growth 31 , 32 , as modified by Tohidi Moghadam et al . 2 , 33 . Briefly, seed solution was prepared by addition of HAuCl 4 .…”

Section: Methodsmentioning

confidence: 99%

“…3H 2 O (400 μL, 0.01 M) and CTAB (9.5 mL, 0.095 M). Finally, 40 μL of seed solution was added and rod-shaped nanostructures were formed after several hours 2 .…”

Section: Methodsmentioning

confidence: 99%

“…cm 2 .dmol −1 , as depicted from the following equation: where, θ is the measured ellipticity in degrees, c is the protein concentration in mg.ml −1 , l is the path length in cm, Mw is the molecular weight of the protein and n is the number of amino acid residues. Data was smoothed and analyzed by Jasco software, after subtracting the buffer contribution from the original protein spectrum 2 .…”

Section: Methodsmentioning

confidence: 99%

“…Amongst gold nanoparticles of different morphologies, the rod shaped nanostructures are considered as a prime choice in many biomedical applications. Gold nanorods (GNRs) have shown outstanding properties over other nanoparticles, such as high stability, high absorption efficiency, tunable absorption in the biological window, and deep penetration of near-infrared light for destruction of tumor tissues 1 , 2 . The tiny nanostructures have the highest surface cross-section compared to other morphologies (i.e.…”

Section: Introductionmentioning

confidence: 99%

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Assembly of Gold Nanorods on HSA Amyloid Fibrils to Develop a Conductive Nanoscaffold for Potential Biomedical and Biosensing Applications

Taheri

Akhtari

Moghadam

et al. 2018

Sci Rep

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Today, Gold Nanorods have promised variety of applications in conjugation with biomolecules of interest. Discovery of functional amyloids has also been highlighted with possible use in designing high performance materials. To exploit dual properties of both Nano and Bio counterparts in new functional materials, this effort has focused on synthesis of a potential hybrid system of Gold nanorods (GNRs) and HSA amyloid fibrils to develop a conductive nanoscaffold. UV-Vis spectroscopy, Thioflavin T (ThT) assay, Far-UV Circular Dichroism (CD) spectropolarimetry, fluorescence and Transmission Electron microscopy were used to characterize formation of the nanostructures and amyloid fibrils. Surface plasmon resonance of GNRs was also monitored upon interaction with HSA amyloid fibrils, showing that the plasmonic component of the hybrid system has maintained its characteristic rod morphology without any perturbations. Analysis of Nyquist plots for the hybrid nanoscaffold showed that the electronic behavior of the hybrid system has been enhanced due to the presence of the assembled GNRs. Results of this investigation highlight the possibility of fabricating hybrid nano-bioscaffolds as promising candidates in versatile biomedical and biosensing applications.

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“…Gold Nanorods were synthesized according to seed mediated growth 31 , 32 , as modified by Tohidi Moghadam et al . 2 , 33 . Briefly, seed solution was prepared by addition of HAuCl 4 .…”

Section: Methodsmentioning

confidence: 99%

“…3H 2 O (400 μL, 0.01 M) and CTAB (9.5 mL, 0.095 M). Finally, 40 μL of seed solution was added and rod-shaped nanostructures were formed after several hours 2 .…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Assembly of Gold Nanorods on HSA Amyloid Fibrils to Develop a Conductive Nanoscaffold for Potential Biomedical and Biosensing Applications

Taheri

Akhtari

Moghadam

et al. 2018

Sci Rep

Self Cite

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show abstract

“…1 Such sensitivity to trace changes in the local refractive index, candidates plasmonic nanoparticles for variety of fruitful applications in nanomedicine and biosensing. 2,3 Upon modifications in synthesis conditions, shape of gold nanoparticles could be directed to form anisotropic nanoparticles of rod morphology. Gold nanorods (GNRs) with unique ability to scatter light in the near infrared region have been nominated as promising theranostic agents for different biomedical applications, such as biomedical imaging, biosensing, drug delivery, cancer therapy, regenerative medicine etc.…”

Section: Introductionmentioning

confidence: 99%

Surface Functionalization of Gold Nanorods Improves Nanostructure Assemblies on Amyloid Fibril Scaffolds

Salimian

Moghadam

Ranjbar

2020

ACSi

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A hybrid scaffold containing gold nanorods and lysozyme amyloid fibrils has been fabricated, and the effect of surface modification on improving nanostructure assembly on the biological template has been investigated. The nanohybrid system was characterized by monitoring surface plasmon resonance bands, dynamic light scattering spectroscopy, Thioflavin-T assay, and transmission electron microscopy. Surface of gold nanorods (GNRs) was modified with polystyrene sulfonate (PSS), and possible difference in assembly of the pristine and modified nanostructures was compared upon interaction with amyloid fibrils. Analysis of transmission electron microscopy showed that changing the surface charge of GNRs with biocompatible polymer improved electrostatic interactions between the nanostructures and amyloid fibril templates. Analysis of cell viability assays also showed that surface functionalization of GNRs remarkably improved biocompatibility of the nanoscaffold. Results of this study encourage utilization of modification strategies to fabricate a new generation of nanoscaffolds with fruitful applications in regenerative medicine.

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Evaluation of the antibacterial activity of selenium nanoparticles and those conjugated with lysozyme against Bacillus cereus spiked in pasteurized skim milk

Elgushi,

Mohamed,

Elbarbary

et al. 2024

Discov Food

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Conformation and activity of lysozyme on binding to two types of gold nanorods: A comparative study

Cited by 22 publications

References 37 publications

Assembly of Gold Nanorods on HSA Amyloid Fibrils to Develop a Conductive Nanoscaffold for Potential Biomedical and Biosensing Applications

Assembly of Gold Nanorods on HSA Amyloid Fibrils to Develop a Conductive Nanoscaffold for Potential Biomedical and Biosensing Applications

Surface Functionalization of Gold Nanorods Improves Nanostructure Assemblies on Amyloid Fibril Scaffolds

Evaluation of the antibacterial activity of selenium nanoparticles and those conjugated with lysozyme against Bacillus cereus spiked in pasteurized skim milk

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