Conformational analysis and vibrational spectroscopic investigation of l-proline–tyrosine (l-Pro–Tyr) dipeptide

Kecel, Serda; Özel, Ayşen E.; Akyüz, Sevim; Çeli̇k, Sefa; Agaeva, G. A.

doi:10.1016/j.molstruc.2011.01.030

Cited by 22 publications

(7 citation statements)

References 22 publications

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“…Raman spectroscopy studies [64] also confirmed that both oligoprolines and oligohydroxyprolines ( Figure 4) form ordered spatial structures. Peptides 1-5 were placed on glass slides and examined using a Renishaw inVia Raman microscope equipped with a 532 nm laser set up in the backscattering geometry.…”

Section: Synthesismentioning

confidence: 67%

Conjugates of Chitosan and Calcium Alginate with Oligoproline and Oligohydroxyproline Derivatives for Potential Use in Regenerative Medicine

et al. 2020

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New materials that are as similar as possible in terms of structure and biology to the extracellular matrix (external environment) of cells are of great interest for regenerative medicine. Oligoproline and oligohydroxyproline derivatives (peptides 2–5) are potential mimetics of collagen fragments. Peptides 2–5 have been shown to be similar to the model collagen fragment (H-Gly-Hyp-Pro-Ala-Hyp-Pro-OH, 1) in terms of both their spatial structure and biological activity. In this study, peptides 2–5 were covalently bound to nonwovens based on chitosan and calcium alginate. Incorporation of the peptides was confirmed by Fourier transform -infrared (FT-IR) and zeta potential measurements. Biological studies (cell metabolic activity by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and Live/Dead assay) proved that the obtained peptide-polysaccharide conjugates were not toxic to the endothelial cell line EA.hy 926. In many cases, the conjugates had a highly affirmative influence on cell proliferation. The results of this study show that conjugates of chitosan and calcium alginate with oligoproline and oligohydroxyproline derivatives have potential for use in regenerative medicine.

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Section: Synthesismentioning

confidence: 67%

Conjugates of Chitosan and Calcium Alginate with Oligoproline and Oligohydroxyproline Derivatives for Potential Use in Regenerative Medicine

et al. 2020

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“…The C‐O stretching vibration, coupled to the OH in‐plane deformation, was observed at 1,232.05 and 1,234.10 cm −1 (Socrates, 2004). Bands near 624.85 and 700.58 cm −1 were assigned to the C = O out‐plane bending vibrations (Anandan et al, 2012; Kecel, Ozel, Akyuz, Celik, & Agaeva, 2011). Bands in the range of 1,100–1,350 cm −1 represented the characteristic absorption peak of the C‐F group.…”

Section: Resultsmentioning

confidence: 99%

A new multistage counter current extraction method of removing fluoride from defatted Antarctic krill powder

Wang

Jiang

et al. 2020

J Food Process Preserv

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This study aims to find an efficient acid extraction method to remove fluoride from defatted krill powder. Considering the effects of acid species, concentration, temperature, extraction time, solvent volume on the defluoridation efficiency, and final product yield, a new multistage counter current extraction method was developed. The optimum reaction condition was obtained by adding 10 ml of 0.3 M HCl to 1 g defatted krill powder and extracting for four stages, each stage was lasting for 60 min. After this process, fluoride content per dry weight of the final product was remarkably reduced to <5 mg/kg, defluoridation efficiency was 99.70%, and water consumption was minimized. Fourier transform infrared spectroscopy also indicated a decrease in the amount of fluorine, and basic chemical composition measurement indicated krill protein was not damaged during this process. Therefore, these results could be used as theoretical guidance for removing fluoride in practical application. Practical applications Defatted Antarctic krill powder is a cheap by‐product after krill oil extraction, but it contains both high‐quality protein and high concentration of fluoride. The toxicity of fluoride in krill powder makes it not be allowed as food resource. So, a new counter current extraction method was developed to defluorinate from defatted krill powder, which was an easy and cheap process, but high defluoridation efficiency with low water consumption. It has been successfully applied to the food pilot base, and it would help to obtain the safe defatted Antarctic krill powder in krill industry.

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“…The high intensity of the asymmetrical peak at 1073 rel. cm −1 , associated with C-C and COH stretches, points to a strong influence of Pro (1075) and Cys (1085) on the spectrum [17,18,20,21]. The small peak at 1122 rel.…”

Section: Raman Spectrum Of Hcx26mentioning

confidence: 97%

Temperature-sensitive gating of hCx26: high-resolution Raman spectroscopy sheds light on conformational changes

Kniggendorf

Meinhardt‐Wollweber

Yuan

et al. 2014

Biomed. Opt. Express

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The temperature-sensitive gating of human Connexin 26 (hCx26) was analyzed with confocal Raman microscopy. High-resolution Raman spectra covering the spectral range between 400 and 1500 rel. cm(-1) with a spectral resolution of 1 cm(-1) were fully annotated, revealing notable differences between the spectrum recorded from solubilized hCx26 in Ca(2+)-buffered POPC at 10°C and any other set of protein conditions (temperature, Ca(2+) presence, POPC presence). Spectral components originating from specific amino acids show that the TM1/EL1 parahelix and probably the TM4 trans-membrane helix and the plug domain are involved in the gating process responsible for fully closing the hemichannel.

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Conformational analysis and vibrational spectroscopic investigation of l-proline–tyrosine (l-Pro–Tyr) dipeptide

Cited by 22 publications

References 22 publications

Conjugates of Chitosan and Calcium Alginate with Oligoproline and Oligohydroxyproline Derivatives for Potential Use in Regenerative Medicine

Conjugates of Chitosan and Calcium Alginate with Oligoproline and Oligohydroxyproline Derivatives for Potential Use in Regenerative Medicine

A new multistage counter current extraction method of removing fluoride from defatted Antarctic krill powder

Temperature-sensitive gating of hCx26: high-resolution Raman spectroscopy sheds light on conformational changes

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