2016
DOI: 10.1088/1758-5090/8/2/025001
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Conformational and functional variants of CD44-targeted protein nanoparticles bio-produced in bacteria

Abstract: Biofabrication is gaining interest as a mean to produce nanostructured functional materials because of its operational versatility and full scalability. Materials based on proteins are especially appealing as protein structure and functionality are adaptable by genetic engineering. Furthermore, strategies and tools for protein production have been steadily developed and refined for more than 30 years. However, protein conformation and therefore activity might be sensitive to production conditions. We have expl… Show more

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Cited by 18 publications
(13 citation statements)
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“…Although endotoxin-free E. coli strains have been developed and appear as highly promising for protein production [ 15 , 16 ], the impact of relevant mutations in these strains on complex protein structures still remains to be fully determined [ 51 ]. In fact, features of both the genetic background of the producing strain as well as details of the purification process significantly affect the molecular organization of fine protein oligomers [ 52 , 53 ].…”
Section: Discussionmentioning
confidence: 99%
“…Although endotoxin-free E. coli strains have been developed and appear as highly promising for protein production [ 15 , 16 ], the impact of relevant mutations in these strains on complex protein structures still remains to be fully determined [ 51 ]. In fact, features of both the genetic background of the producing strain as well as details of the purification process significantly affect the molecular organization of fine protein oligomers [ 52 , 53 ].…”
Section: Discussionmentioning
confidence: 99%
“…Protein integrity was analysed by SDS electrophoresis on TGX Stain-Free gels (Bio-Rad, Hercules,CA, USA) and followed by Western blotting using an anti-His monoclonal antibody (Santa Cruz Biotechnology, Inc., Heidelberg, Germany). Protein concentration was determined by an adapted Bradford's assay [25]. Proteins are found as nanoparticles upon purification, and no specific assembling protocols were applied.…”
Section: Protein Purificationmentioning
confidence: 99%
“…2,4,16 Self-assembling NP, including virus-like particles, can be formed from a number of native proteins with high affinity of binding. [17][18][19] Water-insoluble proteins in hydro-alcoholic solvents with surfactants and phospholipids at optimal pH can also be aggregated in NP 14 that may be stabilized by cross-linking with glutaraldehyde. 11 Thermal denaturation of tobacco mosaic virus was shown to produce NP.…”
Section: Introductionmentioning
confidence: 99%