Conformational behaviour and aggregation of chickpea cystatin in trifluoroethanol: Effects of epicatechin and tannic acid

Bhat, Sheraz Ahmad; Bano, Bilqees

doi:10.1016/j.abb.2014.08.015

Cited by 14 publications

(9 citation statements)

References 58 publications

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“…In the context of the present work, incubation of GBC at pH 3.0 with Kaempferol and Catechin has shown the results which are with the agreements of the previous study with different polyphenols [17]. A number of in vitro studies have used concentration or dose dependent antiaggregation properties of phenolic compounds.…”

Section: Discussionsupporting

confidence: 87%

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Evaluation of polyphenols as possible therapeutics for amyloidoses: Comparative analysis of Kaempferol and Catechin

Bhat

Bano

2015

International Journal of Biological Macromolecules

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Section: Discussionsupporting

confidence: 87%

“…Aliquots drawn from each sample (keeping protein concentration at 10 M) were scanned in a UV-vis spectrophotometer (UV-VIS 1700 Shimadzu, Japan), at 450 nm using a quartz cuvette of 1 cm path length. The increase in the absorbance value at 450 nm is a measure of the scattering of light by aggregated particles [17].…”

Section: Solution Turbidity Measurementmentioning

confidence: 99%

Evaluation of polyphenols as possible therapeutics for amyloidoses: Comparative analysis of Kaempferol and Catechin

Bhat

Bano

2015

International Journal of Biological Macromolecules

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“…Moreover, ANS fluorescence results showed unfolding of α‐crystallin to different degrees, suggesting the formation and existence of structural intermediates and thus signifying tertiary structural changes. TFE‐induced conformational changes and increased hydrophobicity have been also reported for other proteins (Bhat and Bano, ).…”

Section: Discussionsupporting

confidence: 68%

Effect of trifluoroethanol on α‐crystallin: folding, aggregation, amyloid, and cytotoxicity analysis

Khan

Tabrez

Bhat

et al. 2015

J of Molecular Recognition

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α-Crystallin, a member of small heat shock proteins, is the major structural protein within the eye lens and is believed to play an exceptional role in the stability of lens proteins and its transparency. In the current manuscript, we have investigated the effect of an organic solvent, trifluoroethanol (TFE), on the structure and function of α-crystallin isolated from camel eye lens. Incubation of this protein with TFE changed the secondary and tertiary structures, which resulted in the aggregation of α-crystallin as evidenced by intrinsic fluorescence, Rayleigh's scattering, Thioflavin T assay, and circular dichroism spectroscopic studies. The treatment with different concentrations of TFE led to increased exposure of hydrophobic domains of α-crystallin, which was observed by 8-anilino 1-napthalene sulfonic acid extrinsic fluorescence assay. These results clearly indicate that TFE induced significant changes in the secondary and tertiary structures of α-crystallin, leading to aggregation and amyloid formation. Furthermore, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay established the cytotoxicity of the aggregated α-crystallin towards HepG2 cell lines through reactive oxygen species production. In conclusion, α-crystallin protein was found to be susceptible to conformational changes by TFE, suggesting that α-crystallin, although basically acting like a heat shock protein and functionally displaying chaperone-like activity, might capitulate to change in lens environment induced by diseased conditions or age-related changes, resulting in cataract formation.

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“…Among them curcumin, the phenolic (diferuloylmethane) yellow curry pigment, has been suggested to inhibit formation of amyloid fibrils in vitro [15]. Unfortunately there are limited evidences [16] that polyphenols of plant origin might exert some protection towards development of neurodegenerative diseases.…”

Section: Introductionmentioning

confidence: 99%

Role of dietary antioxidant (−)-epicatechin in the development of β-lactoglobulin fibrils

Carbonaro

Venere

Filabozzi³

et al. 2016

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

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Conformational behaviour and aggregation of chickpea cystatin in trifluoroethanol: Effects of epicatechin and tannic acid

Cited by 14 publications

References 58 publications

Evaluation of polyphenols as possible therapeutics for amyloidoses: Comparative analysis of Kaempferol and Catechin

Evaluation of polyphenols as possible therapeutics for amyloidoses: Comparative analysis of Kaempferol and Catechin

Effect of trifluoroethanol on α‐crystallin: folding, aggregation, amyloid, and cytotoxicity analysis

Role of dietary antioxidant (−)-epicatechin in the development of β-lactoglobulin fibrils

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