Conformational change of adsorbed and desorbed bovine serum albumin on nano-sized magnetic particles

Peng, Z.G.; Hidajat, K.; Uddin, M.S.

doi:10.1016/j.colsurfb.2003.08.007

Cited by 74 publications

(39 citation statements)

References 30 publications

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“…1. It showed that the size of magnetic particles was about 10-15 nm, which was similar to the previous reports [17,18]. It is known that magnetic particles less than 30 nm will exhibit superparamagnetism [18].…”

Section: Characterization Of Magnetic Particles and Magnetic Microsphsupporting

confidence: 75%

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Effect of hydrophobic/hydrophilic characteristics of magnetic microspheres on the immobilization of BSA

Zhang

Zhi

et al. 2011

Colloids and Surfaces B: Biointerfaces

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Section: Characterization Of Magnetic Particles and Magnetic Microsphsupporting

confidence: 75%

“…Magnetic particles were prepared by chemical co-precipitation method [17,18]. A complete precipitation of Fe 3 O 4 was achieved under alkaline condition (25% NH 3 H 2 O) maintaining a molar ratio of Fe 2+ :Fe 3+ = 1:1.6.…”

Section: Preparation Of Fe 3 O 4 Magnetic Particlesmentioning

confidence: 99%

Effect of hydrophobic/hydrophilic characteristics of magnetic microspheres on the immobilization of BSA

Zhang

Zhi

et al. 2011

Colloids and Surfaces B: Biointerfaces

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“…These results are in agreement with earlier reported values where the maximum adsorption of 418.9 lg/mg at pH value of 4.64 for Fe 3 O 4 nanoparticles with 10 nm particle size was reported in the presence of carbodiimide [2]. Thus the maximum adsorption of BSA is obtained at pH value of 5.58 for both the NFO particles.…”

Section: Protein Adsorption Analysissupporting

confidence: 96%

“…It has therefore promoted widespread interest in many research areas [1] involving the separation of biomolecules from the organic and inorganic matrix. Here the bio-molecules are attached onto the magnetic particles and then separated from the bulk solution by using magnetic field gradient [2,3]. The main advantage of using magnetic separation for bio-molecules is that the separation process can be used without large changes in the environmental factors [4].…”

Section: Introductionmentioning

confidence: 99%

Micro-structural analysis of NiFe2O4 nanoparticles synthesized by thermal plasma route and its suitability for BSA adsorption

Bhosale

Kanhe

Bhoraskar

et al. 2015

J Mater Sci: Mater Med

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The paper presents the experimental studies pertaining to the adsorption of bovine serum albumin (BSA) on the nanoparticles of nickel ferrite (NiFe2O4) with a view of correlating the adsorption properties to their microstructure and zeta potentials. Physical properties of two kinds of nickel ferrites, one synthesized by thermal plasma route and the other by chemical co-precipitation method, are compared. Maximum adsorption (231.57 μg/mg) of BSA onto nickel ferrite nanoparticles, at body temperature (37 °C) was observed at pH-value of 5.58 for the thermal plasma synthesized particles showing its higher adsorption capacity than those synthesized by wet chemical means (178.71 μg/mg). Under the same physical conditions the value of zeta potential, obtained for the former, was higher than that of the latter over a wide range of pH values (3.64-9.66). This is attributed to the differences in the specific surface energies of the two kinds of nanoparticles arising from the degree of crystallinity. The paper presents the experimental evidence for the single crystalline nature of the individual nanoparticles, with mean size of 32 nm, for the thermal plasma synthesized particles as evidenced from the high resolution transmission electron microscopy and electron diffraction analysis. The measurements also reveal the poor crystalline morphology in the chemically prepared particles (mean size of 28 nm) although the X-ray diffraction patterns are not much different. The atomic force microscopy images confirm that the surfaces of plasma synthesized nanoparticles possesses higher surface roughness than that of chemically synthesized one. Presence of adsorbed protein was confirmed by vibrational spectroscopy. The Langmuir adsorption model is found to fit into the experimental data better than the Freundlich adsorption model.

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“…Pepsin adsorption to the nanoparticle and enzymatic activity was examined even over successive reuse cycles. Peng et al [42] studied the conformational changes of BSA adsorbed on nanoparticles. The structures of native BSA, adsorbed BSA, and desorbed BSA were studied by several methods, circular dichroism, fluorescence spectroscopy, and differential scanning calorimetry.…”

Section: Gold Nanoparticlesmentioning

confidence: 99%

Separation of biomolecules using electrophoresis and nanostructures

Kist¹,

Mandaji²

2004

Electrophoresis

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A large number of nanostructures have the potential to be used together with electrophoresis as separation media or separation additive in capillary electrophoresis, micellar electrokinetic chromatography, capillary electrochromatography, and other analytical techniques. Among those structures are nanotubes, nanocavities, nanowires, nanoposts, nanocones, nanospheres, molecular imprints, nanoparachutes (conical monodendrons), and general nanoparticles with random structures. This review is focused only on publications describing experimental works using molecular imprints, nanoposts, and nanospheres that are fabricated and applied for the purpose of separation media in electrophoresis-driven separations. The review follows an approximate chronological order in each section. As shown, the most popular are those resulting from molecular imprinting technologies. These biomimetic receptors are used in a great variety of fields, which includes electrophoresis, micellar electrokinetic chromatography, capillary electrochromatography, and other fields not reviewed in this work. A few examples of these other fields are, e.g., liquid chromatography, membranes, extractor or preconcentration techniques, immunosorbent assays, and sensing devices. The second topic scanned in the present work is the nanostructures that are used as obstacles to replace gels or polymers solutions in electrophoresis. Finally, the nascent field of nanospheres of gold and other materials as separation media is also reviewed.

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Conformational change of adsorbed and desorbed bovine serum albumin on nano-sized magnetic particles

Cited by 74 publications

References 30 publications

Effect of hydrophobic/hydrophilic characteristics of magnetic microspheres on the immobilization of BSA

Effect of hydrophobic/hydrophilic characteristics of magnetic microspheres on the immobilization of BSA

Micro-structural analysis of NiFe2O4 nanoparticles synthesized by thermal plasma route and its suitability for BSA adsorption

Separation of biomolecules using electrophoresis and nanostructures

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