Conformational Changes of Fibrinogen Adsorption onto Hydroxyapatite and Titanium Oxide Nanoparticles

Chen, Yongli; Zhang, Xiufang; Gong, Yu; Zhao, Nanming; Tingying, Zeng; Xinqi, Song

doi:10.1006/jcis.1999.6159

Cited by 120 publications

(90 citation statements)

References 36 publications

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“…Consequently, the state of HPF adsorbed onto nanosilica is close to the native state. This is in agreement with published results on the adsorption of HPF on a hydrophilic surface with oxidized Si [40] or titania [21]. Consequently, nanosilica is characterized by certain biocompatibility with respect to proteins.…”

Section: Adsorption Of Hpfsupporting

confidence: 93%

“…Carbonyl stretching vibrations of HPF at 1655 cm −1 shifted slightly towards lower wavenumbers (1640-1637 cm −1 ) in comparison with alone HPF (Fig. 4) [21,40]. Upon adsorption, there is an increase in turn structure and a decrease in β-sheet structure.…”

Section: Adsorption Of Hpfmentioning

confidence: 92%

“…1a) or other structures could form the RN-H + · ··O(H)Si≡ bonds. Upon HPF adsorption the content of α-helix slightly decreased [21]. Consequently, peptide bonds could take part in the binding of HPF to the silica surface.…”

Section: Adsorption Of Hpfmentioning

confidence: 98%

“…HPF molecules in the adsorbed state look 'thinner' due to interaction with the surface, which can also result in conformational changes of adsorbed and desorbed proteins [21]. AFM investigations of the HPF adsorption to a silica surface show that HPF molecules mainly adsorb through their D and E globules [22].…”

Section: Introductionmentioning

confidence: 99%

“…AFM investigations of the HPF adsorption to a silica surface show that HPF molecules mainly adsorb through their D and E globules [22]. The HPF adsorption onto titania and hydroxyapatite leaded to a decrease in ordered secondary structure of the protein; the HPF conformation changes further upon washing and desorption, because the α-helix content decreases gradually during the adsorption, washing, and desorption processes [21]. Adsorption of proteins at a hydrophobic surface of silylated silica is irreversible [23].…”

Section: Introductionmentioning

confidence: 99%

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Interaction of fibrinogen with nanosilica

et al. 2007

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Interaction of human plasma fibrinogen (HPF) with fumed nanosilica A-300 in a phosphate buffer solution (PBS) was studied using 1 H NMR spectroscopy with layer-by-layer freezing-out of bulk and interfacial water in the temperature range of 210-273 K, TSDC (90 < T < 265 K), adsorption, FTIR, and UV spectroscopy methods. An increase in concentration of HPF in the PBS leads to a decrease in amounts of structured water (frozen at T < 273 K) because of coagulation of HPF molecules. Addition of nanosilica to the HPF solution strongly reduces the amounts of structured water because of adsorption interaction of HPF molecules with silica nanoparticles, self-association of HPF molecules, formation of denser packed hybrid agglomerates with HPF and silica, and lastly, because of conformational changes of HPF. A monolayer adsorption capacity of A-300 corresponds to 156 mg of HPF per gram of silica. The FTIR and UV spectra show that the HPF adsorption on silica leads to structural changes of the protein molecules. These changes and formation of hybrid HPF/A-300 aggregates can increase the rate of clotting that is of importance on nanosilica application as a component of tourniquet preparations.

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Section: Adsorption Of Hpfsupporting

confidence: 93%

Section: Adsorption Of Hpfmentioning

confidence: 92%

Section: Adsorption Of Hpfmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Interaction of fibrinogen with nanosilica

et al. 2007

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Nano‐Bio‐Chemical Braille for Cells: The Regulation of Stem Cell Responses using Bi‐Functional Surfaces

Chrzanowski

Lee

Kondyurin

et al. 2014

Adv Funct Materials

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Insufficient integration with local host tissues is a significant problem that adversely affects the performance of implanted biomedical devices. Poor tissue integration leaves patients susceptible to complications associated with adverse foreign body reactions and infections that typically mandate expensive and elevated‐risk revision surgery. The aging population and growing incidence of medical implants makes the development of bio‐functional implant surfaces a high priority research imperative. Here multifunctional surfaces are reported that are capable of regulating cell adhesion and triggering cell differentiation to facilitate osseointegration of implantable devices. The approach described is universal, cost‐ and time‐effective. It relies on a unique combination of two advances: i) a reactive interface provided by a plasma activated coating (PAC) that covalently immobilises bioactive molecules with significantly higher efficiency than conventional technologies, and ii) multifunctional molecules (bi‐functional fusion‐proteins) that regulate multiple cellular responses. Covalent linking of the molecules, their high density, and desired orientation are demonstrated. The effectiveness of these functional interfaces to regulate mesenchymal stem cell attachment and differentiation is confirmed suggesting the ability to regulate osseointegration. This method is a leap forward in the fabrication of truly biofunctional materials tailored for particular applications.

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Integrierte Hybridsysteme aus Nanopartikeln und Biomolekülen: Synthese, Eigenschaften und Anwendungen

2004

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Metall‐ oder Halbleiter‐Nanopartikel und Nanostäbchen haben ähnliche Dimensionen wie Proteine (Enzyme, Antigene, Antikörper) oder DNA. Die Integration von Nanopartikeln (mit ihren besonderen elektronischen, photonischen und katalytischen Eigenschaften) mit Biomaterialien (mit ihren einzigartigen Erkennungsmechanismen sowie katalytischen und inhibierenden Fähigkeiten) ergibt hybride Nanobiomaterialien mit synergistischen Eigenschaften und Funktionen. Dieser Aufsatz beschreibt die aktuellsten Entwicklungen bei der Synthese von Hybridsystemen aus Biomolekülen und Nanopartikeln oder Nanostäbchen und deren Anwendung zum Aufbau von geordneten 2D‐ und 3D‐Strukturen in Lösung und auf Oberflächen. Schwerpunkte sind der Einsatz von Biomolekül‐Nanopartikel‐Konjugaten für die Bioanalytik sowie zur Herstellung bioelektronischer Funktionseinheiten und Nanoleiterbahnen aus Metall, Halbleitern oder magnetischen Materialien.

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Conformational Changes of Fibrinogen Adsorption onto Hydroxyapatite and Titanium Oxide Nanoparticles

Cited by 120 publications

References 36 publications

Interaction of fibrinogen with nanosilica

Interaction of fibrinogen with nanosilica

Nano‐Bio‐Chemical Braille for Cells: The Regulation of Stem Cell Responses using Bi‐Functional Surfaces

Integrierte Hybridsysteme aus Nanopartikeln und Biomolekülen: Synthese, Eigenschaften und Anwendungen

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