2014
DOI: 10.1021/ja411633w
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Conformational Dynamics of a Seven Transmembrane Helical Protein Anabaena Sensory Rhodopsin Probed by Solid-State NMR

Abstract: Permanent WRAP URL:http://wrap.warwick.ac.uk/62548 Copyright and reuse:The Warwick Research Archive Portal (WRAP) makes this work by researchers of the University of Warwick available open access under the following conditions. Copyright © and all moral rights to the version of the paper presented here belong to the individual author(s) and/or other copyright owners. To the extent reasonable and practicable the material made available in WRAP has been checked for eligibility before being made available.Copies … Show more

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Cited by 81 publications
(145 citation statements)
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“…We first measured the bulk 15 N T 1ρ as a function of the spinlock amplitude (Figure S9). In agreement with measurements in GB1 and membrane protein ASR,16, 17 we found a relaxation plateau for spinlock fields from about 10 to 25 kHz, yielding a bulk 15 N T 1ρ of approximately 100 ms for the TM part of KcsA. The site‐resolved 15 N T 1ρ relaxation rates (R 1ρ ) show a rather uniform dynamic regime for the lipid‐confined TM part, which is in line with the measurements in micelles18 (Figures 3 A, B).…”
supporting
confidence: 89%
“…We first measured the bulk 15 N T 1ρ as a function of the spinlock amplitude (Figure S9). In agreement with measurements in GB1 and membrane protein ASR,16, 17 we found a relaxation plateau for spinlock fields from about 10 to 25 kHz, yielding a bulk 15 N T 1ρ of approximately 100 ms for the TM part of KcsA. The site‐resolved 15 N T 1ρ relaxation rates (R 1ρ ) show a rather uniform dynamic regime for the lipid‐confined TM part, which is in line with the measurements in micelles18 (Figures 3 A, B).…”
supporting
confidence: 89%
“…Provided that high resolution can be achieved either by selective labeling37 or quasi-crystalline preparation of the membrane protein of interest383940, even side-specific dynamics information such as segmental order parameters can be determined. In the case of G protein-coupled receptors, spectral resolution is limited and only a single case has been reported in the literature where a large amount of sequential assignments have been achieved41.…”
Section: Resultsmentioning
confidence: 99%
“…There is a growing number of interesting dynamics studies that address proteins of increasing complexity and biological interest, such as membrane proteins [104,184,185] and amyloid fibrils [106,186,187]. For reasons of limited space we focus here only on one particular aspect of biomolecular dynamics.…”
Section: Selected Examples Of Recent Applications: How Do Protein Motmentioning
confidence: 99%