Conformational fluctuations in a green fluorescent protein-like Akane family protein: a high-pressure fluorescence study at 0.1–700 MPa

Maeno, Akihiro; Kato, Yuko; Jimbo, Mitsuru; Amada, Kei; Mita, Hajime; Akasaka, Kazuyuki

doi:10.1080/08957959.2017.1293049

Cited by 2 publications

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“…The Akane family also has a rigid structure against high pressure. [ 16 ] To the best of our knowledge, the characteristics mentioned above with pH on Alc‐Orange have never been reported. DsRed's green and red forms do not show an apparent change in green and red emission spectra with pH.…”

Section: Resultsmentioning

confidence: 99%

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Different properties of two types of red fluorescent proteins in octocoral, Scleronephthya spp. as Akane families

et al. 2022

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We report the different properties of two types of red fluorescent proteins (RFP), undescribed species, extracted from two octocorals, Scleronephthya sp. 1 (S. sp. 1) and S. sp, 2 (Alcyonacea, Nephtheidae). S. sp. 1, named Alc-Orange, emits strong green emission at 492 nm and weak red emission at 590 and 630 nm when excited at 449 and 574 nm, respectively. S. sp. 2, LS-Red, emits strong deep red at 642 nm and weak green at 480 and 510 nm when excited at 574 nm and 434 nm, respectively. LS-Red has a very large Stokes shift of about 208 nm emitting at 642 nm when excited at 434 nm. Interestingly, LS-Red shows some emissions at 480 (blue emission), 514 (green emission), 563 (orange emission), and 642 nm (deep red emission) continuously at pH 7.5, which means multicolored fluorescence protein by one excitation at 434 nm. In pH dependence of fluorescence of Alc-Orange (pH 13 to 3.5), no relation between 'green and red FPs' was observed, whereas LS-Red showed the interconversion between 'green and red forms' depending on pH (11.5 to 4.5).

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Section: Resultsmentioning

confidence: 99%

“…It was found that the red brown‐Akane consisting of its β‐can structure is not broken even at 680 MPa, retaining intense green emission at 510 nm and weak red emissions at 590 and 630 nm after returning to 1 MPa at 25°C. [ 16 ]…”

Section: Introductionmentioning

confidence: 99%

Different properties of two types of red fluorescent proteins in octocoral, Scleronephthya spp. as Akane families

et al. 2022

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Characterization of recombinant photoconverting green fluorescent Akanes

Jimbo,

Otake,

Amano

et al. 2023

The Journal of Biochemistry

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Summary Akanes are fluorescent proteins that have several fluorescence maxima. In this report, Akane1 and Akane3 from Scleronephthya gracillima were selected, successfully overexpressed in Escherichia coli, and purified by affinity chromatography. Fluorescence spectra of the recombinant Akanes matured in darkness, or ambient light were found to have several fluorescence peaks. SDS-PAGE analysis revealed that Akanes matured in ambient light have two fragments. MS/MS analysis of Akanes digested with trypsin showed that the cleavage site is the same as observed for the photoconvertible fluorescent protein Kaede. The differences between the calculated masses from the amino acid sequence of Akane1 and the measured masses of Akane1 fragments obtained under ambient light coincided with those of Kaede. In contrast, a mass difference between the measured N-terminal Akane3 fragment and the calculated mass indicated that Akane3 is modified in the N-terminal region. These results indicate that numerous peaks in the fluorescent spectra of Akanes partly arise from isoproteins of Akanes and photoconversion. Photoconversion of Akane1 caused a fluorescence change from green to red, which was also observed for Akane3; however, the fluorescent intensity decreased dramatically when compared with that of Akane3.

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Conformational fluctuations in a green fluorescent protein-like Akane family protein: a high-pressure fluorescence study at 0.1–700 MPa

Cited by 2 publications

References 17 publications

Different properties of two types of red fluorescent proteins in octocoral, Scleronephthya spp. as Akane families

Different properties of two types of red fluorescent proteins in octocoral, Scleronephthya spp. as Akane families

Characterization of recombinant photoconverting green fluorescent Akanes

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