Conformations of different gelatins in solutions and in films an analysis of circular dichroism (CD) measurements

Wetzel, R.; Buder, E.; Hermel, H.; Hüttner, A.

doi:10.1007/bf01417460

Cited by 22 publications

(19 citation statements)

References 56 publications

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“…The triple helical structure of gelatin can be quantified by using CD measurements [15]. In the present study, the CD spectra of gelatin showed two peaks, a negative peak at 205 nm suggesting a random coil conformation, and a positive peak at 222 nm characteristic of the triple-helial conformation of gelatin [16–19].…”

Section: Resultsmentioning

confidence: 74%

Applications of Circular Dichroism for Structural Analysis of Gelatin and Antimicrobial Peptides

Gopal

Park

Seo

et al. 2012

IJMS

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Circular dichroism (CD) is a useful technique for monitoring changes in the conformation of antimicrobial peptides or gelatin. In this study, interactions between cationic peptides and gelatin were observed without affecting the triple helical content of the gelatin, which was more strongly affected by anionic surfactant. The peptides did not adopt a secondary structure in the presence of aqueous solution or Tween 80, but a peptide secondary structure formed upon the addition of sodium dodecyl sulfate (SDS). The peptides bound to the phosphate group of lipopolysaccharide (LPS) and displayed an alpha-helical conformation while (KW) 4 adopted a folded conformation. Further, the peptides did not specifically interact with the fungal cell wall components of mannan or laminarin. Tryptophan blue shift assay indicated that these peptides interacted with SDS, LPS, and gelatin but not with Tween 80, mannan, or laminarin. The peptides also displayed antibacterial activity against P. aeruginosa without cytotoxicity against HaCaT cells at MIC, except for HPA3NT3-analog peptide. In this study, we used a CD spectroscopic method to demonstrate the feasibility of peptide characterization in numerous environments. The CD method can thus be used as a screening method of gelatin-peptide interactions for use in wound healing applications. OPEN ACCESSInt. J. Mol. Sci. 2012, 13 3230

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Section: Resultsmentioning

confidence: 74%

Applications of Circular Dichroism for Structural Analysis of Gelatin and Antimicrobial Peptides

Gopal

Park

Seo

et al. 2012

IJMS

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“…However, presumably the involvement of hydrogen bonds is lower in dried films, in which most of the water has been removed and the storage temperature is around 20°C, which may act to cause the differences in imino acid content between the two gelatins to be less appreciable. In this connection, the mechanical properties of gelatin films have been related to the triple helix content of the gelatin employed (Bigi et al, 2004); however, it is known to decrease upon isothermal dehydration of films (Wetzel et al, 1987).…”

Section: Characterization Of the Filmsmentioning

confidence: 99%

Physico-chemical and film-forming properties of bovine-hide and tuna-skin gelatin: A comparative study

Gómez-Estaca¹,

Montero²,

Fernández-Martı́n³

et al. 2009

Journal of Food Engineering

142

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“…However, this difference was not enough to impart significantly higher strength to the mammalian-gelatin films, probably because the stabilizing role of water molecules and cold temperature is less important in the films than it is in the hydrogels. In this connection, the triple helical structure 10 content has been shown to decrease upon isothermal dehydration of films (Wetzel, Buder, Hermel & Huttner, 1987). In addition, the larger amounts of β components in fish gelatins could also appreciably strengthen the corresponding films.…”

mentioning

confidence: 93%

Fish gelatin: a renewable material for developing active biodegradable films

Gómez‐Guillén

Pérez‐Mateos

Gómez-Estaca

et al. 2009

Trends in Food Science & Technology

430

208

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Most films used to preserve foodstuffs are made from synthetic plastic materials.However, for environmental reasons, attention has recently turned to biodegradable films. Gelatin has been extensively studied for its film forming capacity and applicability as an outer covering to protect food against drying, light, and oxygen. Moreover, it is 5 one of the first materials proposed as a carrier of bioactive components. Gelatins from alternatives to mammalian species are gaining prominence, especially gelatins from marine fish species. Because of their good film-forming abilities, fish gelatins may be a good alternative to synthetic plastics for making films to preserve foodstuffs. The mechanical and barrier properties of these films depend largely on the physical and 10 chemical characteristics of the gelatin, especially the amino acid composition, which is highly species specific, and the molecular weight distribution, which depends mainly on processing conditions. Different film formulations can be developed to extend the films' physical and chemical properties and to add new functional attributes. This paper reviews the most recent scientific literature dealing with films based on gelatins from 15 different fish species and considers various strategies intended to improve the physical properties of such films by combining fish gelatins with such other biopolymers as soy protein isolate, oils and fatty acids, and certain polysaccharides. The use of plasticizers and cross-linking agents is also discussed. Specific attributes, such as antimicrobial and antioxidant activities, may be also conferred by blending the gelatin 20 with chitosan, lysozyme, essential oils, plant extracts, or vitamin C to produce an active packaging biomaterial.

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Conformations of different gelatins in solutions and in films an analysis of circular dichroism (CD) measurements

Cited by 22 publications

References 56 publications

Applications of Circular Dichroism for Structural Analysis of Gelatin and Antimicrobial Peptides

Applications of Circular Dichroism for Structural Analysis of Gelatin and Antimicrobial Peptides

Physico-chemical and film-forming properties of bovine-hide and tuna-skin gelatin: A comparative study

Fish gelatin: a renewable material for developing active biodegradable films

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