Conformity of Fine Needle Aspiration Biopsy (FNAB) and Core Needle Biopsy (CNB) in peripheral lung tumor patients: A cross-sectional study

Marhana, Isnin Anang; Widianiti, Kadek; Kusumastuti, Etty Hary

doi:10.1016/j.amsu.2022.103423

Cited by 6 publications

(8 citation statements)

References 19 publications

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“…Specimens from the periphery of the chest cavity were obtained by core biopsies using ultrasonography or computed tomography (CT) guidance, while specimens from the central chest cavity were acquired by forceps biopsies and bronchoalveolar lavage. The aforementioned methods are considerably safe and feasible to use in collecting specimens for pathological or molecular diagnosis (Marhana et al 2022). However, these methods are quite invasive and can result in difficulties, such as too few specimens or insufficient exfoliated cells for pathological diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Expression of Melanoma Antigen Genes A11 and A12 in Non-Small Cell Lung Cancer

Gondo Mastutik,

Alphania Rahniayu,

Isnin Anang Marhana

et al. 2023

FMI

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Highlights:1. In this study, new primers designed using the semi-nested polymerase chain reaction (PCR) method were utilized to identify MAGE-A11 and MAGE-A12 expressions in specimens collected from core biopsy, forcep biopsy, and bronchoalveolar lavage.2. The histopathological analysis revealed positive expressions of MAGE-A11 and MAGE-A12 in specimens diagnosed with non-small cell lung cancer (NSCLC) as well as in specimens with no malignant cells. AbstractThe melanoma antigen gene (MAGE) belongs to the group of cancer testis antigens that are exclusively expressed in germ cells but may be re-expressed in cancer cells. The highly expressed MAGE-A subfamily in lung cancer may potentially be a diagnostic and prognostic biomarker. This study aimed to identify MAGE-A11 and MAGE-A12 expressions in lung tumors obtained from core biopsy, forceps biopsy, and bronchoalveolar lavage specimens. A cross-sectional observational study was conducted on 90 patients clinically diagnosed with lung tumors. These patients received core biopsy, forceps biopsy, and bronchoalveolar lavage interventions after ethical approval was obtained. The complementary deoxyribonucleic acid (cDNA) quality was assessed by the polymerase chain reaction (PCR) of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The assessment was performed to ascertain if all specimens exhibited positive PCR amplification of the GAPDH gene. MAGE-A11 and MAGE-A12 were identified through a semi-nested reverse transcription PCR. The positive results were detected by measuring the PCR products, with MAGE-A11 and MAGE-A12 measuring at base pairs (bp) of 858 and 496 in the first and second rounds, respectively. The expressions of MAGE-A11 and MAGE-A12 were observed in 3 (3.33%) and 40 (44.44%) out of 90 specimens, respectively. The prevalence rate of non-small cell lung cancer (NSCLC) was 31.11% (28/90). Among these cases, 3.57% (1/28) showed the expression of MAGE-A11, while 32.14% (9/28) exhibited the expression of MAGE-A12. Sixty-two (68.89%) out of 90 patients were diagnosed with no tumor cell malignancy. Out of 62 cases, 2 (3.23%) exhibited the expression of MAGE-A11, while 31 (50%) demonstrated the expression of MAGE-A12. MAGE-A11 and MAGE-A12 were detected in NSCLC and in certain specimens with a pathological diagnosis that indicated the absence of malignant cells. In conclusion, MAGE A11 and MAGE A12 have potential markers that can improve the pathological diagnosis of lung cancer. Further investigation is necessary to explore the expression of MAGE-A in correlation with lung cancer progression.

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Section: Discussionmentioning

confidence: 99%

Expression of Melanoma Antigen Genes A11 and A12 in Non-Small Cell Lung Cancer

Gondo Mastutik,

Alphania Rahniayu,

Isnin Anang Marhana

et al. 2023

FMI

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“…It is because patients with lung cancer do not show clear symptoms at an early stage. The new symptoms appear and can only be felt after cancer has reached the advanced stage and patients have a poor prognosis [ 3 , 5 ]. In addition, the life expectancy of lung cancer sufferers is very low, only about 16% survive for up to 5 years despite receiving treatment, including surgery [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, CT-guided is commonly used to diagnose peripheral lung cancer [ 7 ]. For central lung tumors, lung tissue collection is usually carried out using bronchoscopy, such as forceps biopsy (FB) or bronchoalveolar lavage (BAL) [ 5 ]. The FB has been performed to collect the lung specimens to diagnose malignancy with minimal complications such as minor bleeding that could be spontaneously resolved or treated using ice-cold saline or an agent for vasoconstriction [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…In general, bronchoscopy with FB and BAL is a safe technique and allows for obtaining specimens for histopathological examination [ 4 ]. However, this method is invasive [ 6 , 9 ] and sometimes encounters obstacles [ 5 ], such as inadequate cell number for a histopathological diagnosis. In addition, there is also an increased risk of bleeding in the very fragile cancerous tissue [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

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Expression of MAGE A1 to MAGE A10 in the Forceps Biopsy and Bronchoalveolar Lavage Specimens from Patients with the Central Lung Tumor

Mastutik,

Rahniayu,

Marhana

et al. 2024

Asian Pac J Cancer Prev

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Objective: The objective was to evaluate the expression of the MAGE A subtypes family in the central lung tumor patients from the forceps biopsy (FB) and bronchoalveolar lavage (BAL) specimens and to analyze its association with the histopathological examination. Methods: An observational study was conducted on 32 FB and 43 BAL specimens from patients with central lung tumors. All samples were assessed for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression by reverse transcription (RT) polymerase chain reaction (PCR) and samples showing a positive result were examined for MAGE A subtypes family expression by nested-RT PCR. Result: The MAGE A1 to MAGE A10 genes were highly expressed in the FB and BAL specimens from patients with central lung tumors. The MAGE A1 to MAGE A10 gene and MAGE A1 to MAGE A6 gene were expressed in 60/75 (80%) and 16/75 (21.3 %), respectively. MAGE A8 , MAGE A9 , and MAGE A10 were the most commonly expressed. In FB specimens diagnosed without malignant cells, MAGE A1 to MAGE A10 and MAGE A1 to MAGE A6 were positive in 16/18 (88.9 %) and 1/18 (5.6 %), respectively. In all BAL specimens were diagnosed with no malignant cells, but MAGE A1 to MAGE A10 and MAGE A1 to MAGE A6 showed positive results in 36/43 (83.7%) and 9/43 (20.9%) %), respectively. There was a significant association between MAGE A1 to MAGE A6 expression with histopathological diagnosis. Conclusion: The MAGE A subtype family genes are highly expressed in central lung tumor patients from FB and BAL specimens, even in specimens that were diagnosed with no malignant cells. All BAL specimens were diagnosed as no malignant cells, but expression of the MAGE A subfamily genes was found in more than 80% of the specimens. These observations suggest that combining histopathological and molecular examination could improve the diagnosis of lung malignancy.

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“…Terakhir membuat hapusan, fiksasi dan melakukan pulasan. Jika diperlukan, prosedur ini dapat diulang untuk kedua atau ketiga kalinya sesuai dengan penilaian awal oleh ahli Patologi Anatomi [6]…”

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Prototipe Lung Tumor e-mannequin untuk Pelatihan Keterampilan Fine Needle Aspiration Biopsy

Retnani,

Anita,

Sulistyarini

et al. 2024

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Kanker paru merupakan penyebab kematian nomer 2 setelah kanker payudara dengan insiden 11,4% dari penyakit keganasan. Pengambilan spesimen untuk menegakkan diagnosis kanker paru oleh dokter spesialis Patologi Anatomik dapat dilakukan melalui prosedur Fine Needle Aspiration Biopsy (FNAB) menggunakan jarum halus melalui tuntunan imaging dari USG atau CT scan. Berbasis integrasi teknologi 3D Printer dengan manekin berbasis elektronik peneliti melakukan penelitian eksperimental dengan tujuan membuat e-mannequin yang dilengkapi dengan tumor paru untuk pelatihan keterampilan prosedur FNAB oleh mahasiswa Pendidikan Dokter Spesialis Patologi Anatomik sebelum melakukan tindakan pada pasien. Pembuatan e-mannequin menggunakan bahan filamen, tumor berbahan Thermoplastic Polyurethane (TPU) diletakkan pada lokasi paru kiri dan kanan dengan konsistensi kenyal yang dapat dimasukkan jarum halus dan dipasang sensor lampu sebagai validator ketepatan tindakan FNAB pada tumor paru.

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Conformity of Fine Needle Aspiration Biopsy (FNAB) and Core Needle Biopsy (CNB) in peripheral lung tumor patients: A cross-sectional study

Cited by 6 publications

References 19 publications

Expression of Melanoma Antigen Genes A11 and A12 in Non-Small Cell Lung Cancer

Expression of Melanoma Antigen Genes A11 and A12 in Non-Small Cell Lung Cancer

Expression of MAGE A1 to MAGE A10 in the Forceps Biopsy and Bronchoalveolar Lavage Specimens from Patients with the Central Lung Tumor

Prototipe Lung Tumor e-mannequin untuk Pelatihan Keterampilan Fine Needle Aspiration Biopsy

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