Confounding Factors in Bioassays with Freshwater and Marine Organisms

Postma, J.F.; Valk, S. de; Dubbeldam, Marco; Maas, J.L.; Tonkes, Marcel; Schipper, C.A.; Kater, Belinda J.

doi:10.1006/eesa.2002.2195

Cited by 68 publications

(44 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ammonia and conductivity are confounding factors that may interfere with the bioeffects of micropollutants. Postma et al 2002 indicate that the highest values at which they observed no significant effects were ammonia 13-60 mg L -1 and conductivity \650 lS cm -1 . On this basis, ammonia and conductivity in our study (0.73 mg L -1 and 143.2 lS cm -1 , respectively) are not confounding factors.…”

Section: Discussionmentioning

confidence: 87%

Oxidative stress induced in Hyalella azteca by an effluent from a NSAID-manufacturing plant in Mexico

et al. 2016

View full text Add to dashboard Cite

Production in the pharmaceutical industry has increased and along with it, the amount of wastewater of various characteristics and contaminant concentrations. The main chemicals in these effluents are solvents, detergents, disinfectants-such as sodium hypochlorite (NaClO)-and pharmaceutical products, all of which are potentially ecotoxic. Therefore, this study aimed to evaluate the oxidative stress induced in the amphipod Hyalella azteca by the effluent from a nonsteroidal anti-inflammatory drug (NSAID)-manufacturing plant. The median lethal concentration (72 h-LC50) was determined and H. azteca were exposed to the lowest observed adverse effect level (0.0732 %) for 12, 24, 48 and 72 h, and biomarkers of oxidative stress were evaluated [hydroperoxide content (HPC), lipid peroxidation (LPX), protein carbonyl content (PCC), and the activity of the superoxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)]. Statistically significant increases with respect to the control group (P < 0.05) were observed in HPC, LPX and PCC in H. azteca at all exposure times. Antioxidant enzymes activity SOD, CAT and GPx activity also increased significantly (P < 0.05) with respect to the control group. In conclusion, the industrial effluent analyzed in the present study contains NSAIDs and NaClO, and induces oxidative stress in H. azteca.

show abstract

Section: Discussionmentioning

confidence: 87%

Oxidative stress induced in Hyalella azteca by an effluent from a NSAID-manufacturing plant in Mexico

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Several factors may complicate the interpretation of sediment tests, including uncertainty (Vorhees et al, 2002) and confounding factors (e.g., grain size, ammonia, sulfides, indigenous animals) (Postma et al, 2002). Grain size, for instance, is a well-known confounding factor in sediment tests (US EPA, 1994; US EPA/ACE, 2001) that can cause amphipod mortality by physical or energetic stress when outside species-specific tolerance ranges (Emery et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

A comparison of acute and chronic toxicity methods for marine sediments

Kennedy

Steevens

Lotufo

et al. 2009

Marine Environmental Research

View full text Add to dashboard Cite

Sediment toxicity tests are valuable tools for assessing the potential effects of contaminated sediments in dredged material evaluations because they inherently address complexity (e.g., unknown contaminants, mixtures, bioavailability). Although there is a need to understand the chronic and sublethal impacts of contaminants, it is common to conduct only short-term lethality tests in evaluations of marine sediments. Chronic toxicity methods for marine sediments have been developed but the efficacy of these methods is less documented. In this evaluation of marine sediments collected from the New York/New Jersey (NY/NJ) Harbor, three 10-d acute toxicity test methods (Ampelisca abdita, Leptocheirus plumulosus, Americamysis bahia) and three chronic and sublethal test methods (28-d L. plumulosus, 20- and 28-d Neanthes arenaceodentata) were applied by three testing laboratories. Although the N. arenaceodentata and A. bahia tests did not indicate significant toxicity for the sediments tested in this study, these methods have been reported useful in evaluating other sediments. The 10-d A. abdita, 10-d L. plumulosus and 28-d L. plumulosus tests were comparable between laboratories, indicating 29-43%, 29%, and 43-71% of the tested sediments as potentially toxic. The 28-d L. plumulosus method was the only chronic toxicity test that responded to the test sediments in this study. The 28-d L. plumulosus endpoint magnitudes were related to sediment chemistry and the sublethal endpoints were reduced as much or more than acute lethality endpoints. However, intra-treatment sublethal endpoint variability was greater, compromising detection of statistical significance. In this study, the chronic L. plumulosus test method was less consistent among laboratories relative to acute test methods, identifying potential for toxicity in a similar number (or slightly more) NY/NJ Harbor sediments.

show abstract

“…l N was never significantly lower than l control ), and while millimolar concentrations of NH 4 + have been shown to inhibit the growth of some marine bacteria (Toussaint et al, 1995;Kadam and Boone, 1996;Postma et al, 2002), the concentration used in this study was in the micromolar range and is typical of many coastal oceans. Generally, bacterial growth was limited by inorganic N during AMT16 (May/June) and co-limited by organic C and inorganic N during AMT17 (October/November) (Fig.…”

Section: Inorganic Limitation and Co-limitation Of Bacterial Growthmentioning

confidence: 96%

Meridional patterns of inorganic nutrient limitation and co-limitation of bacterial growth in the Atlantic Ocean

Hale

Rivkin

2017

Progress in Oceanography

View full text Add to dashboard Cite

a b s t r a c tGrowth of heterotrophic bacteria is generally considered to be controlled by temperature and the availability of organic substrates, however there is evidence that bacterial growth can also be limited by the concentrations or supply rate of inorganic nutrients (i.e. nitrogen, phosphorus or iron). We examined spatial and seasonal patterns of organic carbon and inorganic nutrient (N and P) limitation of bacterial growth along each of two meridional transects through the Atlantic Ocean, during contrasting seasons.Here we used nutrient bioassays to demonstrate widespread inorganic nutrient limitation and colimitation with organic carbon in the oligotrophic temperate, tropical and subtropical ocean. There were distinct seasonal and spatial differences in the inorganic and organic nutrient limitation of bacterial growth, with inorganic nitrogen as the primary limiting nutrient in May/June, and inorganic nitrogen and organic carbon co-limiting growth in October/November. There was no evidence that the availability of inorganic phosphorus limited bacterial growth in the Southern Hemisphere. We propose that the patterns of nutrient-dependent bacterial growth reflect seasonal and spatial differences in aeolian inputs and the quality of dissolved organic matter, and that bacteria directly compete with autotrophs for inorganic nutrients in the oligotrophic regions of the World Ocean. The findings of this study have important implications for understanding the balance between the biological and microbial carbon pumps, and the modelling of the net metabolic balance of the Ocean in response to climate-driven changes in nutrient inputs.Crown

show abstract

Confounding Factors in Bioassays with Freshwater and Marine Organisms

Cited by 68 publications

References 44 publications

Oxidative stress induced in Hyalella azteca by an effluent from a NSAID-manufacturing plant in Mexico

Oxidative stress induced in Hyalella azteca by an effluent from a NSAID-manufacturing plant in Mexico

A comparison of acute and chronic toxicity methods for marine sediments

Meridional patterns of inorganic nutrient limitation and co-limitation of bacterial growth in the Atlantic Ocean

Contact Info

Product

Resources

About