Congenital
            <i>Trypanosoma cruzi</i>
            Infection in Neonates and Infants from Two Regions of Chile Where Chagas' Disease Is Endemic

Jercic, María Isabel; Mercado, Rubén; Villarroel, Rodrigo

doi:10.1128/jcm.00048-10

Cited by 11 publications

(5 citation statements)

References 8 publications

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“…In Argentina the transmission rate is estimated between 2–12% [ 12 ]. In Chile, in two of the endemic regions (IV and V regions), the congenital transmission rate of the parasite is 8.4% [ 13 ]. According to WHO/PAHO the number of infected women at fertile age is approximately 1.8 million and it is estimated that 14,400 neonates are being infected each year [ 14 ], this is another reason why this form of transmission becomes epidemiologically more important.…”

Section: Congenital Chagas Diseasementioning

confidence: 99%

Mechanism ofTrypanosoma cruziPlacenta Invasion and Infection: The Use of Human Chorionic Villi Explants

Fretes

Kemmerling

2012

Journal of Tropical Medicine

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Congenital Chagas disease, a neglected tropical disease, endemic in Latin America, is associated with premature labor and miscarriage. During vertical transmission the parasite Trypanosoma cruzi (T. cruzi) crosses the placental barrier. However, the exact mechanism of the placental infection remains unclear. We review the congenital transmission of T. cruzi, particularly the role of possible local placental factors that contribute to the vertical transmission of the parasite. Additionally, we analyze the different methods available for studying the congenital transmission of the parasite. In that context, the ex vivo infection with T. cruzi trypomastigotes of human placental chorionic villi constitutes an excellent tool for studying parasite infection strategies as well as possible local antiparasitic mechanisms.

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Section: Congenital Chagas Diseasementioning

confidence: 99%

Mechanism ofTrypanosoma cruziPlacenta Invasion and Infection: The Use of Human Chorionic Villi Explants

Fretes

Kemmerling

2012

Journal of Tropical Medicine

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“…Transmission is mainly through hematophagous insect vectors (Brisse et al 2000;Teixeira et al 2006), although other transmission mechanisms exist, such as congenital infection (Jercic et al 2010) and by ingestion of food contaminated with infective forms of the parasite (Ramírez et al 2013). Due to the migration of infected persons, this disease is spread to non-endemic regions through transfusion and congenital transmission (Jannin and Villa 2007).…”

Section: Introductionmentioning

confidence: 99%

Expression, purification, and biochemical characterization of recombinant DNA polymerase beta of the Trypanosoma cruzi TcI lineage: requirement of additional factors and detection of phosphorylation of the native form

et al. 2015

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Chagas disease, caused by the protozoan Trypanosoma cruzi, is a major parasitic disease that affects millions of people in America. However, despite the high impact of this disease on human health, no effective and safe treatment has been found that eliminates the infecting parasite from human patients. Among the possible chemotherapeutic targets that could be considered for study in T. cruzi are the DNA polymerases, in particular DNA polymerase beta (polß), which previous studies have shown to be involved in kinetoplast DNA replication and repair. In this paper, we describe the expression, purification, and biochemical characterization of the Miranda clone polß, corresponding to lineage T. cruzi I (TcI). The recombinant enzyme purified to homogeneity displayed specific activity in the range described for a highly purified mammalian polß. However, the trypanosome enzyme exhibited important differences in biochemical properties compared to the mammalian enzymes, specifically an almost absolute dependency on KCl, high sensitivity to N-ethylmaleimide (NEM), and low sensitivity to ddTTP. Immuno-affinity purification of T. cruzi polymerase beta (Tcpolß) from epimastigote extracts showed that the native enzyme was phosphorylated. In addition, it was demonstrated that Tcpolß interacts with some proteins in a group of about 15 proteins which are required to repair 1-6 bases of gaps of a double strand damaged DNA. It is possible that these proteins form part of a DNA repair complex, analogous to that described in mammals and some trypanosomatids.

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“…prevalence increases dramatically when a higher number of FTA sections are analyzed (Cox et al 2010). Further studies should consider analyzing multiple FTA sections and a complementary PCR protocol that targets host DNA to avoid false-negatives (Molaei et al 2008; Jercic et al 2010; Schijman et al 2011). …”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology of Trypanosomatids and Trypanosoma cruzi in Primates from Peru

Aysanoa

Mayor

Mendoza³

et al. 2017

EcoHealth

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We determined the prevalence rate and risk of infection of Trypanosoma cruzi and other trypanosomatids in Peruvian non-human primates (NHPs) in the wild (n = 126) and in different captive conditions (n = 183). Blood samples were collected on filter paper, FTA cards, or EDTA tubes and tested using a nested PCR protocol targeting the 24Sα rRNA gene. Main risk factors associated with trypanosomatid and T. cruzi infection were genus and the human–animal context (wild vs captive animals). Wild NHPs had higher prevalence of both trypanosomatids (64.3 vs 27.9%, P < 0.001) and T. cruzi (8.7 vs 3.3%, P = 0.057), compared to captive NHPs, suggesting that parasite transmission in NHPs occurs more actively in the sylvatic cycle. In terms of primate family, Pitheciidae had the highest trypanosomatid prevalence (20/22, 90.9%) and Cebidae had the highest T. cruzi prevalence (15/117, 12.8%). T. cruzi and trypanosomatids are common in Peruvian NHPs and could pose a health risk to human and animals that has not been properly studied.

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Congenital Trypanosoma cruzi Infection in Neonates and Infants from Two Regions of Chile Where Chagas' Disease Is Endemic

Cited by 11 publications

References 8 publications

Mechanism ofTrypanosoma cruziPlacenta Invasion and Infection: The Use of Human Chorionic Villi Explants

Mechanism ofTrypanosoma cruziPlacenta Invasion and Infection: The Use of Human Chorionic Villi Explants

Expression, purification, and biochemical characterization of recombinant DNA polymerase beta of the Trypanosoma cruzi TcI lineage: requirement of additional factors and detection of phosphorylation of the native form

Molecular Epidemiology of Trypanosomatids and Trypanosoma cruzi in Primates from Peru

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