Congruence of community structure between taxonomic identification and T-RFLP analyses in free-living soil nematodes

George, Paul B. L.; Lindo, Zoë

doi:10.1016/j.pedobi.2015.04.003

Cited by 2 publications

(2 citation statements)

References 33 publications

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“…One limitation of PCR‐based molecular methods is that not actual abundances of the specimen but rather their relative number of DNA copies are assessed (Porazinska et al, ; Waite et al, ). However, there is recent evidence that molecular methods might give similar ecological patterns as traditional methods (Geisen et al, ; George & Lindo, ; Hamilton, Strickland, Wickings, Bradford, & Fierer, ; Porazinska, Sung, Giblin‐Davis, & Thomas, ; Quist et al, ). Hence, amplicon sequencing has high potential to assess soil management effects on nematode communities across multiple field experiments.…”

Section: Introductionmentioning

confidence: 99%

Reduced tillage, but not organic matter input, increased nematode diversity and food web stability in European long‐term field experiments

et al. 2019

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Soil nematode communities and food web indices can inform about the complexity, nutrient flows and decomposition pathways of soil food webs, reflecting soil quality. Relative abundance of nematode feeding and life‐history groups are used for calculating food web indices, i.e., maturity index (MI), enrichment index (EI), structure index (SI) and channel index (CI). Molecular methods to study nematode communities potentially offer advantages compared to traditional methods in terms of resolution, throughput, cost and time. In spite of such advantages, molecular data have not often been adopted so far to assess the effects of soil management on nematode communities and to calculate these food web indices. Here, we used high‐throughput amplicon sequencing to investigate the effects of tillage (conventional vs. reduced) and organic matter addition (low vs. high) on nematode communities and food web indices in 10 European long‐term field experiments and we assessed the relationship between nematode communities and soil parameters. We found that nematode communities were more strongly affected by tillage than by organic matter addition. Compared to conventional tillage, reduced tillage increased nematode diversity (23% higher Shannon diversity index), nematode community stability (12% higher MI), structure (24% higher SI), and the fungal decomposition channel (59% higher CI), and also the number of herbivorous nematodes (70% higher). Total and labile organic carbon, available K and microbial parameters explained nematode community structure. Our findings show that nematode communities are sensitive indicators of soil quality and that molecular profiling of nematode communities has the potential to reveal the effects of soil management on soil quality.

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Section: Introductionmentioning

confidence: 99%

Reduced tillage, but not organic matter input, increased nematode diversity and food web stability in European long‐term field experiments

et al. 2019

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“…Higher PPI calculated values than MI values means that plant-parasitic nematode populations dominated the community of free-living nematodes (bacterivores, fungivores, and omnivores). Bacterivorous and fungivorous nematodes contribute to soil fertility (Ferris & Matute, 2003;Neher, 2001;Chen & Ferris, 2000;Ingham et al, 1985), therefore soils that create microenvironments conducive for the proliferation of bacterial and fungal feeding nematodes are desirable (Matute & Anders, 2012). Also, omnivorous nematodes are suppressive to pest species, for example the plant-parasitic nematodes (Matute et al, 2013;Matute, 2013).…”

Section: Ecological Functionsmentioning

confidence: 99%

Nematode Composition and Soil Conditions in Plots under a Wheat Crop in Colfax, Washington State

Matute¹,

Carter²,

Sherman³

2015

JAS

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Plant parasitic nematodes have been identified as a major problem to wheat (Triticum aestivum L.) production in the Pacific Northwest (PNW) states of the USA. Nematicide control of these parasites has been successful. We were, however, interested in understanding how the soil nematode-nematode interactions and the nematode-host plant interactions affect nematode populations of different feeding groups and how the soil nematode composition could be used to elucidate soil conditions in a winter wheat crop. Our results revealed that the ratio of the plant-parasite index (PPI) to the maturity index (MI = free-living nematodes) was high, meaning we had higher populations of the herbivorous nematodes than all of the other feeding groups combined. The low populations of the free-living nematodes meant little or no nitrogen mineralization by the bacterivorous and fungivorous nematodes, and also a lack of meaningful predation of the plant-parasitic nematodes by the pest suppressive species i.e omnivores and carnivores. The high plant-parasitic nematode populations and low populations of the free-living nematodes may have also interfered with nutrient uptake by the plants and resulted in stressed soils in the plots studied. Based on our results, farming methods that will reverse the PPI/MI ratio will be a natural and ecologically friendly way of reducing populations of the parasitic nematodes in the wheat plots e.g. rotations and soil amendments. During the different sampling periods, the soil conditions changed from basal, to enriched, and then maturing, based on the nematode composition. Thus the soil nematode composition can be used for the biomonitoring of soil conditions in wheat plots.

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Congruence of community structure between taxonomic identification and T-RFLP analyses in free-living soil nematodes

Cited by 2 publications

References 33 publications

Reduced tillage, but not organic matter input, increased nematode diversity and food web stability in European long‐term field experiments

Reduced tillage, but not organic matter input, increased nematode diversity and food web stability in European long‐term field experiments

Nematode Composition and Soil Conditions in Plots under a Wheat Crop in Colfax, Washington State

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