Coniferyl alcohol metabolism in conifers — I. Glucosidic turnover of cinnamyl aldehydes by UDPG: coniferyl alcohol glucosyltransferase from pine cambium

Steeves, Valerie; Förster, Hartmut; Pommer, U.; Savidge, Rodney Arthur

doi:10.1016/s0031-9422(01)00107-8

Cited by 44 publications

(40 citation statements)

References 51 publications

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“…In the control cultures of KT root line CA was detected, also after hydrolysis and in this case probably in the form of coniferin which is considered to be a storage or transported form of CA (Savidge 1989;Whetten and Sederoff 1995) hydrolyzed by specific β-glucosidase to CA (Steevens at al. 2001).…”

Section: Lignan Accumulation In Control Cultures Of Atma and Kt Hairymentioning

confidence: 99%

Lignan accumulation in two-phase cultures of Taxus x media hairy roots

Sykłowska-Baranek

Łysik

Jeziorek

et al. 2018

Plant Cell Tiss Organ Cult

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The biosynthetic potential for six lignans accumulation in two lines of Taxus x media hairy roots was investigated. The cultures of KT and ATMA hairy root lines were supplemented with precursors: coniferyl alcohol (CA 1, 10 or 100 µM) and/or l-phenylalanine (100 µM PHEN) and/or methyl jasmonate (100 µM MeJa). Moreover the two-phase in vitro cultures supported with perfluorodecalin (PFD) as a gas carrier and in situ extrahent were used. The hairy root lines differed in lignan production profiles. In the control untreated cultures KT roots did not accumulate secoisolariciresinol and lariciresinol while ATMA roots did not accumulate matairesinol. In ATMA roots the treatment with CA (1 or 10 µM) resulted in the production of lariciresinol and secoisolariciresinol whereas solely lariciresinol was present after 100 µM CA application. Elicitation with 1 µM CA and MeJa yielded with hydroxymatairesinol aglyca and lariciresinol glucosides with their highest content 37.88 and 3.19 µg/g DW, respectively. The stimulatory effect of simultaneous treatment with 1 µM CA, PHEN and MeJa on lignan production was observed when the cultures were supplemented with PFD-aerated or degassed. In ATMA root cultures these applied conditions were the most favourable for matairesinol content which amounted to 199.86 and 160.25 µg/g DW in PFD-aerated and PFD-degassed supported cultures, respectively. In KT root cultures solely, hydroxymatairesinol and coniferin/CA content was enhanced with their highest yield 59.29 and 134.60 µg/g DW in PFD-aerated and PFD-degassed cultures, respectively.

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Section: Lignan Accumulation In Control Cultures Of Atma and Kt Hairymentioning

confidence: 99%

Lignan accumulation in two-phase cultures of Taxus x media hairy roots

Sykłowska-Baranek

Łysik

Jeziorek

et al. 2018

Plant Cell Tiss Organ Cult

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“…Ralph et al [5] emphasized the need for more research on monolignol transport mechanisms, since lignin formation is controlled by the supply of the monomers to the lignifying zones. Phenylpropanoid glycosides [38] such as coniferin and syringin (glycosides of coniferyl and syringyl alcohols) have been synthesized [39] and also been isolated in high amounts from plant tissues [38], leading to suggestions that these compounds might be the storage and/or transport forms of the corresponding monolignols [12]. Glucosyl transferases [38] and β-glucosidases might regulate the availability of monolignols during lignification [40,41].…”

Section: Monolignol Transportmentioning

confidence: 99%

“…Phenylpropanoid glycosides [38] such as coniferin and syringin (glycosides of coniferyl and syringyl alcohols) have been synthesized [39] and also been isolated in high amounts from plant tissues [38], leading to suggestions that these compounds might be the storage and/or transport forms of the corresponding monolignols [12]. Glucosyl transferases [38] and β-glucosidases might regulate the availability of monolignols during lignification [40,41]. Cell wall-associated β-glucosidases might release the aglycone (monolignols) for subsequent polymerization during lignin synthesis.…”

Section: Monolignol Transportmentioning

confidence: 99%

Supramolecular Self-Assembled Chaos: Polyphenolic Lignin’s Barrier to Cost-Effective Lignocellulosic Biofuels

et al. 2010

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Phenylpropanoid metabolism yields a mixture of monolignols that undergo chaotic, non-enzymatic reactions such as free radical polymerization and spontaneous selfassembly in order to form the polyphenolic lignin which is a barrier to cost-effective lignocellulosic biofuels. Post-synthesis lignin integration into the plant cell wall is unclear, including how the hydrophobic lignin incorporates into the wall in an initially hydrophilic milieu. Self-assembly, self-organization and aggregation give rise to a complex, 3D network of lignin that displays randomly branched topology and fractal properties. Attempts at isolating lignin, analogous to archaeology, are instantly destructive and nonrepresentative of in planta. Lack of plant ligninases or enzymes that hydrolyze specific bonds in lignin-carbohydrate complexes (LCCs) also frustrate a better grasp of lignin. Supramolecular self-assembly, nano-mechanical properties of lignin-lignin, ligninpolysaccharide interactions and association-dissociation kinetics affect biomass deconstruction and thereby cost-effective biofuels production. OPEN ACCESSMolecules 2010, 15 8642

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“…The MLGs are considered to be storage and transport form of the monolignols, and the glucosidation and deglucosidation steps may regulate lignin biosynthesis, while the former is mediated by a uridine diphosphate glucose (UDP-glucose): monolignol glucosyltransferase and the latter is induced by 4-O-β-D-glucosidase (Ibrahim and Grisebach 1976;Ibrahim 1977;Terazawa et al 1984;Dharmawardhana et al 1995;Dharmawardhana and Ellis 1998;Steeves et al 2001;Samuels et al 2002;Lanot et al 2006;Tsuyama and Takabe 2015).…”

Section: Introductionmentioning

confidence: 99%

Monolignol glucosides as intermediate compounds in lignin biosynthesis. Revisiting the cell wall lignification and new ¹³C-tracer experiments with Ginkgo biloba and Magnolia liliiflora

Terashima¹,

Matsushita

et al. 2016

Holzforschung

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Monolignol glucosides as intermediate compounds in lignin biosynthesis. Revisiting the cell wall lignification and new 13C-tracer experiments with Ginkgo biloba and Magnolia liliiflora DOI 10.1515/hf-2015-0224 Received October 17, 2015 accepted February 9, 2016; previously published online March 14, 2016 Abstract: A large amount of monolignol glucosides (MLGs: p-glucocoumaryl alcohol, coniferin, syringin) are found in lignifying soft xylem near cambium and they disappear with the progress of lignification. Recently, it became a matter of debate whether those MLGs are real intermediates in lignin biosynthesis or only a storage form of monolignols outside of the main biosynthetic pathway. The latter is partly based on a misinterpretation of 14 C-tracer experiments and partly on the simple generalization of the results of gene manipulation experiments concerning the flexible and complex lignification. In the present paper, it could be confirmed by the most reliable 13 C-tracer method that MLGs are real intermediates in the pathway from l-phenylalanine to macromolecular ligninpolysaccharides complexes in the cell walls. This pathway via MLGs is essential for transport and programmed delivery of specific monolignols in a stable form from intracellular space to specific lignifying sites within the cell wall. The pool size of MLGs is large in most gymnosperm trees and some angiosperm species that emerged in an early stage of phylogeny, while the pool size is small in most angiosperms. This difference in pool size is reasonably understandable from the viewpoint of plant evolution, in the course of which the role of MLGs changed to meet variation in type of major cells, reaction wood formation, and postmortem lignification.

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Coniferyl alcohol metabolism in conifers — I. Glucosidic turnover of cinnamyl aldehydes by UDPG: coniferyl alcohol glucosyltransferase from pine cambium

Cited by 44 publications

References 51 publications

Lignan accumulation in two-phase cultures of Taxus x media hairy roots

Lignan accumulation in two-phase cultures of Taxus x media hairy roots

Supramolecular Self-Assembled Chaos: Polyphenolic Lignin’s Barrier to Cost-Effective Lignocellulosic Biofuels

Monolignol glucosides as intermediate compounds in lignin biosynthesis. Revisiting the cell wall lignification and new ¹³C-tracer experiments with Ginkgo biloba and Magnolia liliiflora

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