Conjugated linoleic acid modulation of cell membrane in leukemia cells

Agatha, Gerhard; Voigt, Astrid; Kauf, E; Zintl, F

doi:10.1016/j.canlet.2003.11.022

Cited by 41 publications

(37 citation statements)

References 26 publications

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“…1, 2 ). As we could defrom other groups showing that this CLA isomer in particular is well metabolized up to CFA 20:3 and 20:4 ( 14,29,30 ). In contrast, the t9, t11-CLA isomer is only elongated to CFA 20:2 and CFA 22:2, but it is not desaturated.…”

Section: Discussionmentioning

confidence: 56%

“…Concerning CLA metabolism, it has been shown that c9,t11 -CLA incorporates into phospholipids of leukemia cells and can be found in plasma and cellular lipids of healthy men supplemented with a diet enriched with this CLA isomer ( 14,15 ). However, so far the effects of c9,t11 -CLA on individual phospholipid classes, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), and its cellular distribution are not precisely described, and data on the metabolism of the t9,t11 -CLA isomer are completely unavailable.…”

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confidence: 99%

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Differential effects of conjugated linoleic acid isomers on macrophage glycerophospholipid metabolism

Ecker

Liebisch

Scherer

et al. 2010

Journal of Lipid Research

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This article is available online at http://www.jlr.org ( c9,. Many CLA isomers have been described; however, the major isomer occurring in meat and dairy products is c9,t11 -CLA ( 2-4 ). It is produced in the rumen through microbial biohydrogenation of linoleic acid and in tissues by delta-9 desaturation of rumen-derived transvaccenic acid ( t11 -FA 18:1) ( 5 ). In contrast, t9,t11 -CLA is the predominant isomer found in dietary oils, as it is generated during partial hydrogenation of vegetable oils and oil refi ning ( 6, 7 ). Several data from in vitro and animal studies show that dietary CLAs are benefi cial and infl uence the progression of several diseases, including cardiovascular and infl ammatory diseases and cancer ( 8-11 ).We have previously reported that two isomers in particular, c9,t11 -and t9,t11 -CLA, have specifi c and even contrasting effects on gene expression associated with lipid metabolism of human macrophages ( 12, 13 ). Concerning CLA metabolism, it has been shown that c9,t11 -CLA incorporates into phospholipids of leukemia cells and can be found in plasma and cellular lipids of healthy men supplemented with a diet enriched with this CLA isomer ( 14, 15 ). However, so far the effects of c9,t11 -CLA on individual phospholipid classes, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), and its cellular distribution are not precisely described, and data on the metabolism of the t9,t11 -CLA isomer are completely unavailable.Therefore we investigated c9,t11 -CLA and t9,t11 -CLA metabolism by quantitative lipid mass spectrometry. As a cellular model, human primary monocyte-derived macrophages were chosen because these cells are highly active with respect to fatty acid and phospholipid metabolism ( 16 ). Moreover macrophages, which are multifunctional cells present in all tissues of the human body, play important roles in several metabolic diseases, including atheroAbstract Conjugated linoleic acids (CLA) are dietary fatty acids. Whereas cis-9,trans-11-(c9,t11)-CLA can be found in meat and dairy products, trans-9,trans-11-(t9,t11) -CLA is a constituent of vegetable oils. Previous studies showed that these two isomers activate different nuclear receptors and, thus, expression of genes related to lipid metabolism. Here we show that these CLA isomers are differentially elongated and desaturated in primary monocyte-derived macrophages isolated from healthy volunteers by using gas chromatographymass spectrometry (GC-MS). We further demonstrate that c9,t11 -CLA incorporates in phosphatidylcholine (PC) and phosphatidylethanolamine (PE) species and activates de novo glycerophospholipid synthesis by quantitative electrospray ionization-tandem mass spectrometry (ESI-MS/MS). c9,t11 -CLA leads to strong shifts of the species profi les to PC 18:2/18:2 and PE 18:2/18:2, which are due to de novo synthesis and fatty acid remodeling. In contrast, t9,t11 -CLA is preferentially bound to neutral lipids, including triglycerides and cholesterol esters.Taken together our results show that ...

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Section: Discussionmentioning

confidence: 56%

mentioning

confidence: 99%

Differential effects of conjugated linoleic acid isomers on macrophage glycerophospholipid metabolism

Ecker

Liebisch

Scherer

et al. 2010

Journal of Lipid Research

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“…In the human leukemia cells, the supplementation with CLA resulted in the accumulation of the corresponding fatty acid with conjugated double bonds by desaturation/elongation system [26]. However, a little amount of metabolites of CLA with long-chain and/or higher degree of unsaturation was detected in the total lipids of human colon cancer cells (Caco-2) supplemented with CLA (200 µM) for 72 h incubation.…”

Section: Discussionmentioning

confidence: 99%

“…Agatha et al [26] reported that c9,t11-CLA and c9,c11-CLA supplemented in the culture medium was readily incorporated and esterified into a human leukemia cellular phospholipids in a concentration-and time-dependent manner. The cellular phospholipids contained high level of CLA (range: 32-63 g/100g total phospholipids).…”

Section: Discussionmentioning

confidence: 99%

Potent inhibitory effect of trans9, trans11 isomer of conjugated linoleic acid on the growth of human colon cancer cells

Beppu

Hosokawa

Tanaka

et al. 2006

The Journal of Nutritional Biochemistry

122

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This study compared the growth inhibitory effects of pure conjugated linoleic acid (CLA) isomers (cis(c)9,c11-CLA, c9,trans(t)11-CLA, t9,t11-CLA, and t10,c12-CLA) on human colon cancer cell lines (Caco-2, HT-29, and DLD-1). When Caco-2 cells were incubated up to 72 h with 200 M each isomer even in the presence of 10% fetal bovine serum (FBS), cell proliferation was inhibited by all CLA isomers in a time dependent manner. The strongest inhibitory effect was shown by t9,t11-CLA, followed by t10,c12-CLA, c9,c11-CLA, and c9,t11-CLA, respectively. The strongest effect of t9,t11-CLA was also observed in other colon cancer cell lines (HT-29 and DLD-1). The order of the inhibitory effect of CLA isomer was confirmed in the presence of 1% FBS. CLA isomers supplemented in the culture medium were readily incorporated into the cellular lipids of Caco-2 and changed their fatty acid composition. The CLA contents in cellular lipids were 26.2+2.7% for t9,t11-CLA, 35.9+0.3% for c9,t11-CLA, and 46.3+0.8% for t10,c12-CLA, respectively. DNA fragmentation was clearly recognized in Caco-2 cells treated with t9,t11-CLA. This apoptotic effect of t9,t11-CLA was dose-and time-dependent. DNA fragmentation was also induced by 9c,11t-CLA and t10,c12-CLA. However, fragmentation levels with both isomers were much lower than that with t9,t11-CLA.t9t11-CLA treatment of Caco-2 cells decreased Bcl-2 levels in association with apoptosis, whereas Bax levels remained unchanged. These results suggest that decreased expression of Bcl-2 by t9t11-CLA might increase the sensitivity of cells to lipid peroxidation and to programmed cell death, apoptosis. AbstractThis study compared the growth inhibitory effects of pure conjugated linoleic acid (CLA) isomers (cis(c)9,c11-CLA, c9,trans(t)11-CLA, t9,t11-CLA, and t10,c12-CLA) on human colon cancer cell lines (Caco-2, HT-29, and DLD-1). When Caco-2 cells were incubated up to 72 h with 200 µM each isomer even in the presence of 10% fetal bovine serum (FBS), cell proliferation was inhibited by all CLA isomers in a time dependent manner. The strongest inhibitory effect was shown by t9,t11-CLA, followed by t10,c12-CLA, c9,c11-CLA, and c9,t11-CLA, respectively. The strongest effect of t9,t11-CLA was also observed in other colon cancer cell lines (HT-29 and DLD-1). The order of the inhibitory effect of CLA isomer was confirmed in the presence of 1% FBS. * Manuscript 2CLA isomers supplemented in the culture medium were readily incorporated into the cellular lipids of Caco-2 and changed their fatty acid composition. The CLA contents in cellular lipids were 26.2+2.7% for t9,t11-CLA, 35.9+0.3% for c9,t11-CLA, and 46.3+0.8% for t10,c12-CLA, respectively. DNA fragmentation was clearly recognized in Caco-2 cells treated with t9,t11-CLA. This apoptotic effect of t9,t11-CLA was doseand time-dependent. DNA fragmentation was also induced by 9c,11t-CLA and t10,c12-CLA. However, fragmentation levels with both isomers were much lower than that with t9,t11-CLA. t9t11-CLA treatment of Caco-2 cells decreased Bcl-2 levels in...

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“…No entanto, este composto também possui efeito inibitório sobre o crescimento de outros tipos de células cancerígenas, como as leucêmicas, as de câncer de próstata e gástrico. Tanto o isômero trans-10, cis-12 quanto o cis-9, trans-11, por mecanismos distintos, possuem efeito antiproliferativo sobre estes tipos de cânceres, mecanismos estes que envolvem controle do ciclo celular, indução da apoptose e controle do metabolismo do ácido araquidônico [55][56][57][58][59] .…”

Section: Cla E Câncerunclassified

Os efeitos do ácido linoléico conjugado no metabolismo animal: avanço das pesquisas e perspectivas para o futuro

2008

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Conjugated linoleic acid modulation of cell membrane in leukemia cells

Cited by 41 publications

References 26 publications

Differential effects of conjugated linoleic acid isomers on macrophage glycerophospholipid metabolism

Differential effects of conjugated linoleic acid isomers on macrophage glycerophospholipid metabolism

Potent inhibitory effect of trans9, trans11 isomer of conjugated linoleic acid on the growth of human colon cancer cells

Os efeitos do ácido linoléico conjugado no metabolismo animal: avanço das pesquisas e perspectivas para o futuro

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