Conjugated Network Supporting Highly Surface-Exposed Ru Site-Based Artificial Antioxidase for Efficiently Modulating Microenvironment and Alleviating Solar Dermatitis

Abstract

Solar dermatitis, a form of acute radiation burn that affects the skin, results from overexposure to ultraviolet B (UVB) radiation in strong sunlight. Cell damage caused by the accumulation of reactive oxygen species (ROS) produced by UVB radiation plays an important role in UVBinduced inflammation in the skin. Here, for efficiently scavenging excess ROS, modulating the microenvironment, and alleviating solar dermatitis, a π-conjugated network polyphthalocyanine supporting a highly surfaceexposed Ru active site-based artificial antioxidase (HSE-PPcRu) is designed and fabricated with excellent ROS-scavenging, antioxidant, and antiinflammatory capabilities. In photodamaged human keratinocyte cells, HSE-PPcRu could modulate mitogen-activated protein kinase (MAPK) and nuclear factor kappa-B signaling pathways, prevent DNA damage, suppress apoptosis, inhibit pro-inflammatory cytokine secretion, and alleviate cell damage. In vivo animal experiments reveal the higher antioxidant and antiinflammatory efficacies of HSE-PPcRu by reversing the activation of p38 and c-Jun N-terminal kinase, inhibiting expression of cyclooxygenase-2, interleukin-6, interleukin-8, and tumor necrosis factor-α. This work not only provides an idea for alleviating solar dermatitis via catalytically scavenging ROS and modulating the microenvironment but also offers a strategy to design an intelligent conjugated network-based artificial antioxidase with a highly surface-exposed active site.

High-performance colorimetric sensor based on PtRu bimetallic nanozyme for xanthine analysis

“…As shown in Fig. S6a, the absorbance of Pt 91 Ru 9 @C 3 N 4 /TMB/H 2 O 2 remained nearly unchanged upon the addition of sodium azide (NaN 3 ), while a noticeable decline was observed after the introduction of p-benzoquinone (p-BQ), indicating superoxide radical ( 1 O 2 ) as the active intermediate in Pt 91 Ru 9 @C 3 N 4 /H 2 O 2 ( Yang, Bi, et al, 2024 ; Yang, Yuan, et al, 2024 ). Terephthalic acid (TA) was then utilized for additional confirmation of the presence of hydroxyl radicals (·OH) ( Zhou, Chen, et al, 2022 ; Zhou, Wang, et al, 2022 ).…”

“…The UV–vis absorbances at 652 nm were measured 30 s post-reaction. Finally, the K m and V max values were obtained through double-reciprocal Lineweaver-Burk plots using the following equation ( Chai et al, 2023 ; Yang, Yuan, et al, 2024 ): where V 0 denotes the initial rate, V max is the maximal reaction velocity, [ S ] refers to the substrate concentration, and K m denotes the Michaelis constant.…”