Conjugated polymer sensitized hyperbranched titanium dioxide based photoelectrochemical biosensor for detecting AFP in serum

Mo, Xinxin; Wang, Yu; Xiao, Qiyou; Zhou, Xitong; Li, He

doi:10.1016/j.surfin.2021.101103

Cited by 14 publications

(6 citation statements)

References 23 publications

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“…Table 1 summarizes the comparison between AFP detection performance using different electrodes through photoelectrochemical (PEC), electrochemical (EC), or electrochemiluminescence (ECL) detection [43][44][45][46][47][48]. The LOD of the fabricated sensor is lower than that obtained using the AFP antibody/conjugated polymers/titanium dioxide/fluorine-doped tin oxide conductive glass electrode (Ab/CP/TiO 2 /FTO) [43], sandwich analysis based on poly-(2-hydroxyethyl methacrylate)-g-graphene/the second antibody/AFP/BSA/the first antibody/gold nanoparticle-reduced graphene oxide-modified GCE (P(VT-co-HEMA)-g-GO/Ab 2 /AFP/BSA/Ab 1 /AuNPs-rGO/GCE) [44], metal-organic framework-antibody/ AFP/BSA/aptamer-SH/gold nanoparticle/flexible polydimethylsiloxane-modified GCE (MOF-Ab/AFP/BSA/Apt-SH/Au/PDMS/GCE) [45], or antibody/carbodiimide hydrochloride-N-hydroxysuccinimide/ferroferric oxide/carboxylated carbon nanotubes/gold nanoparticlemodified GCE (Ab/EDC-NHS/Fe 3 O 4 /MWCNTs-COOH/AuNPs/GCE) [46] but higher than that obtained on AFP/BSA/AFP antibody/reduced graphene oxide@gold nanoparticle @Ru(bpy) 3 2+ -doped silica nanoparticle-modified GCE (AFP/BSA/Ab/rGO@Au@Ru-SiO2/GCE) [47] and fluorescent microspheres based on 4-vinylphenylboronic acid and divinylbenzene-AFP/AFP antibody/Ti, N/Mg co-doped carbon dot-modified GCE (Poly (DVB-Co-PBA)-AFP/Ab/Ti,Mg@N-CDs/GCE) [48]. Compared with other electrodes that need antibody/DNA to be fixed at the recognition interface or sandwich detection modes, the homogeneous electrochemical sensor constructed by this method has the advantages of simple preparation and high sensitivity.…”

Section: Electrochemical Determination Of Afp Using the Fabricated Ho...mentioning

confidence: 99%

Label-Free Homogeneous Electrochemical Aptasensor Based on Size Exclusion/Charge-Selective Permeability of Nanochannel Arrays and 2D Nanorecognitive Probe for Sensitive Detection of Alpha-Fetoprotein

Zhang,

Liu

et al. 2023

Molecules

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The labeling-free and immobilization-free homogeneous aptamer sensor offers advantages including simple operation, low cost, and high sensitivity, demonstrating great potential in rapid detection of tumor biomarkers in biological samples. In this work, a labeling-free and immobilization-free homogeneous aptamer sensor was conveniently fabricated by combining size exclusion and charge-selective penetration of a nanochannel-modified electrode and two-dimensional (2D) nanorecognition probe which can realize selective and highly sensitive detection of alpha-fetoprotein (AFP) in serum. Vertically ordered mesoporous silica film (VMSF) with ultra-small, uniform, and vertically aligned nanochannels was easily grown on the simple, low-cost, and disposable indium tin oxide (ITO) electrode. Through π-π interaction and electrostatic force, the AFP aptamer (Apt) and electrochemical probe, tris(bipyridine)ruthenium(II) (Ru(bpy)32+), were coloaded onto graphene oxide (GO) through simple incubation, forming a 2D nanoscale recognition probe (Ru(bpy)32+/Apt@GO). Owing to the size exclusion effect of VMSF towards the 2D nanoscale probe, the electrochemical signal of Ru(bpy)32+/Apt@GO could not be detected. In the presence of AFP, the specific binding of AFP to the aptamer causes the dissociation of the aptamer and Ru(bpy)32+ from GO, resulting in their presence in the solution. The efficient electrostatic enrichment towards Ru(bpy)32+ by negatively charged VMSF allows for high electrochemical signals of free Ru(bpy)32+ in the solution. Linear determination of AFP ranged from 1 pg/mL to 1000 ng/mL and could be obtained with a low limit of detection (LOD, 0.8 pg/mL). The high specificity of the adapter endowed the constructed sensor with high selectivity. The fabricated probe can be applied in direct determination of AFP in serum.

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Section: Electrochemical Determination Of Afp Using the Fabricated Ho...mentioning

confidence: 99%

Label-Free Homogeneous Electrochemical Aptasensor Based on Size Exclusion/Charge-Selective Permeability of Nanochannel Arrays and 2D Nanorecognitive Probe for Sensitive Detection of Alpha-Fetoprotein

Zhang,

Liu

et al. 2023

Molecules

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“…In addition, in high-risk but asymptomatic populations, AFP detection indicators are vital for identifying early curable tumors and reducing disease-related mortality in a cost-effective manner. The development of AFP detection technology with high sensitivity is necessary for the early detection of some cancers and the clinical detection of AFP [ 1 , 2 , 3 , 4 ]. To this end, a variety of methods for detecting AFP have been developed, such as enzyme-linked immunosorbent assay [ 5 ], radioimmunoassay [ 6 ], fluorescence immunoassay [ 7 ], electrochemiluminescence [ 8 ], Raman spectroscopy [ 9 ], electrochemical immunosensing [ 10 ], and so on.…”

Section: Introductionmentioning

confidence: 99%

Detection of Alpha Fetoprotein Based on AIEgen Nanosphere Labeled Aptamer Combined with Sandwich Structure of Magnetic Gold Nanocomposites

et al. 2023

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As a biomarker, alpha-fetoprotein (AFP) is valuable for detecting some tumors in men, non-pregnant women, and children. However, the detection sensitivity in some methods needs to be improved. Therefore, developing a simple, reliable, and sensitive detection method for AFP is important for non-malignant diseases. An aptamer binding was developed based on aggregation-induced emission luminogen (AIEgen) nanosphere labeled with Fe3O4@MPTMS@AuNPs. AFP was detected with a sandwich structure of AuNPs magnetic composite particles. An aggregation-induced emission (AIE) molecule and polystyrene (PS) nanosphere complex were assembled, enhancing the fluorescence and improving the sensitivity of detection. The limit of detection (LOD) was at a given level of 1.429 pg/mL, which can best be achieved in serum samples. Finally, the results obtained showed the complex to be promising in practical applications.

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“…Chitosan, a biodegradable material renowned for its superior biocompatibility, exhibits a high degree of compatibility with silver and cerium oxide. Moreover, the porous nature of chitosan enhances the electrode's surface area available for loading, resulting in an amplified response signal [17].…”

Section: Introductionmentioning

confidence: 99%

A simple, sensitive, label-free electrochemical immunosensor based on the chitosan-coated silver/cerium oxide (CS@Ag/CeO₂) nanocomposites for the detection of alpha-fetoprotein (AFP)

et al. 2023

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Metal oxide-based sensors have the benefit of inexpensive, quick response, and high sensitivity in detecting specific biological species. In this article, a simple electrochemical immunosensor was fabricated using antibody-chitosan coated silver/cerium oxide (Ab-CS@Ag/CeO2) nanocomposites on a gold electrode for sensitive alpha-fetoprotein (AFP) diagnosis in human serum samples. Successfully synthesis of AFP antibody-CS@Ag/CeO2 conjugates was confirmed through FTIR spectra of the product. The amine coupling bond chemistry was then used to immobilize the resultant conjugate on a gold electrode surface. It was observed that the interaction of the synthesized Ab-CS@Ag/CeO2 nanocomposites with AFP prevented an electron transfer and reduced the voltammetric Fe(CN)63-/4- peak current, which was proportional to the amount of AFP. The linear ranges of AFP concentration were found from 10-12-10-6 gmL-1. The limit of detection was calculated using the calibration curve and came out to be 0.57pgmL-1. The designed label-free immunosensor successfully detected AFP in human serum samples. As a result, the resulting immunosensor is a promising sensor plate form for AFP detection and could be used in clinical bioanalysis.

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Conjugated polymer sensitized hyperbranched titanium dioxide based photoelectrochemical biosensor for detecting AFP in serum

Cited by 14 publications

References 23 publications

Label-Free Homogeneous Electrochemical Aptasensor Based on Size Exclusion/Charge-Selective Permeability of Nanochannel Arrays and 2D Nanorecognitive Probe for Sensitive Detection of Alpha-Fetoprotein

Label-Free Homogeneous Electrochemical Aptasensor Based on Size Exclusion/Charge-Selective Permeability of Nanochannel Arrays and 2D Nanorecognitive Probe for Sensitive Detection of Alpha-Fetoprotein

Detection of Alpha Fetoprotein Based on AIEgen Nanosphere Labeled Aptamer Combined with Sandwich Structure of Magnetic Gold Nanocomposites

A simple, sensitive, label-free electrochemical immunosensor based on the chitosan-coated silver/cerium oxide (CS@Ag/CeO₂) nanocomposites for the detection of alpha-fetoprotein (AFP)

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Conjugated polymer sensitized hyperbranched titanium dioxide based photoelectrochemical biosensor for detecting AFP in serum

Cited by 14 publications

References 23 publications

Label-Free Homogeneous Electrochemical Aptasensor Based on Size Exclusion/Charge-Selective Permeability of Nanochannel Arrays and 2D Nanorecognitive Probe for Sensitive Detection of Alpha-Fetoprotein

Label-Free Homogeneous Electrochemical Aptasensor Based on Size Exclusion/Charge-Selective Permeability of Nanochannel Arrays and 2D Nanorecognitive Probe for Sensitive Detection of Alpha-Fetoprotein

Detection of Alpha Fetoprotein Based on AIEgen Nanosphere Labeled Aptamer Combined with Sandwich Structure of Magnetic Gold Nanocomposites

A simple, sensitive, label-free electrochemical immunosensor based on the chitosan-coated silver/cerium oxide (CS@Ag/CeO2) nanocomposites for the detection of alpha-fetoprotein (AFP)

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A simple, sensitive, label-free electrochemical immunosensor based on the chitosan-coated silver/cerium oxide (CS@Ag/CeO₂) nanocomposites for the detection of alpha-fetoprotein (AFP)