Conjugates of Synthetic Lymphocyte-Activating Lipopeptides with Segments from HIV Proteins Induce Protein-Specific Antibody Formation

Loleit, Manuel; Tröger, Wilfried; Wiesmüller, Karl‐Heinz; Jung, Günther; Strecker, M; Bessler, Wolfgang G.

doi:10.1515/bchm3.1990.371.2.967

Cited by 27 publications

(9 citation statements)

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“…After staining the gel with Coomassie Blue, HMG protein bands were cut from the gel and electroeluted with a Biotrap apparatus (Schleicher & Schuell, Dassel, FRG). Immunization of Balb/c mice with 10-20 lag of this pure HMG protein and preparation of the antisera were performed according to Loleit et al (1990).…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of high-mobility-group proteins from maize

Grasser¹,

Wurz²,

Feix³

1991

Planta

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Chromosomal nonhistone high-mobility-group (HMG) proteins were purified from nuclei of maize (Zea mays L. cv. A619) endosperm and leaf tissue. Tissuespecific differences were observed in their polypeptide patterns, in in-vitro phosphorylation experiments with a casein-kinase type II, and by Western blot analysis with antisera against different HMG proteins. Gelfiltration chromatography demonstrated that maize HMG proteins occur as monomers. By measuring the capacity of the HMG proteins to bind to the 5' flanking region of a zein gene, the sensitivity of the proteins to different temperatures, salt concentrations and pH values was determined.

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Section: Methodsmentioning

confidence: 99%

Isolation and characterization of high-mobility-group proteins from maize

Grasser¹,

Wurz²,

Feix³

1991

Planta

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“…It has recently been shown that association of either HIV-I gp160 or influenza virus hemagglutinin with lipid structures [22,63,64] permits efficient triggering of both virus-specific neutralizing Ab and Cd8+ CTL. Use of immune stimulating complexes [22] or lipopeptide derivatives [64] as immunogens should make it possible to enter the endogeneous and exogenous pathways of antigen processing in order to induce both Tand B cell immunity.…”

Section: Biommentioning

confidence: 99%

“…Use of immune stimulating complexes [22] or lipopeptide derivatives [64] as immunogens should make it possible to enter the endogeneous and exogenous pathways of antigen processing in order to induce both Tand B cell immunity.…”

Section: Biommentioning

confidence: 99%

Identification of a major human immunodeficiency virus‐1 reverse transcriptase epitope recognized by mouse CD4⁺ T lymphocytes

Haas

David

Frank³

et al. 1991

Eur J Immunol

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Delineation of major T helper cell recognition sites of human immunodeficiency virus (HIV-1) proteins represents one important step in the design of an efficient acquired immune deficiency syndrome (AIDS) vaccine. Towards this end, we have explored the immunogenicity of HIV-1BRU proteins in the mouse model. Preliminary experiments revealed that inbred mice primed with whole inactivated HIV-1 developed strong CD4+ T cell proliferative responses to a variety of recombinant viral proteins including reverse transcriptase (RT). To characterize further the mouse T cell responses to this protein, several Ad- or Ed-restricted T hybridoma cells (THC) were established from BALB/c or DBA/2 mice. These THC were tested for their capacity to recognize a series of 15-mer synthetic overlapping peptides spanning three segments of HIV-1 RT that had been preselected on the basis of either alpha-helicity, amphipaticity, and/or for containing rare amino acid sequence patterns. Peptides corresponding to a C-terminal region (residues 528-560) of RT were recognized by several of the THC established from RT-primed mice. Furthermore, a non-alpha-helical peptide from this region (A3, 528-543) was capable of priming mice with different H-2 haplotypes for both peptide A3 and native RT CD4+ T cell recognition. In addition to the recently identified RT determinant 203-219 capable of triggering both mouse and human CD8+ CTL, the present results identify a good candidate for an immunodominant RT epitope capable of eliciting RT-specific T helper cell responses.

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“…In contrast to the protocols mentioned above, haptens or low‐molecular‐weight antigens were covalently linked to the non‐immunogenic, synthetic lipopeptides, which served as carriers with built‐in adjuvant activity, and these conjugates were used in immunization studies. High antibody titres were obtained often after only one immunization [1], [5–7]. The same approach was also used for the production of human monoclonal antibodies after in vitro immunization with antigen–adjuvant conjugates [8].…”

Section: Introductionmentioning

confidence: 99%

Induction of an epitope-specific humoral immune response by lipopeptide—hapten conjugates: enhancement of the anti-melittin response by a synthetic T helper (T_h)-cell epitope

Hoffmann

Loleit

Mittenbühler

et al. 1997

FEMS Immunol Med Microbiol

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Lipopeptides of bacterial origin constitute potent immunoadjuvants when combined with antigens. After the immunization with lipopeptides covalently coupled to non-immunogenic low-molecular-mass antigens or haptens, a hapten-specific humoral immune response can often be obtained. The response against synthetically prepared melittin fragments was further enhanced by the additional introduction of a T helper (Th)-cell epitope into the lipopeptide-hapten conjugate. The Th-cell epitope applied, which is presented by the MHC class II molecule of the BALB/c (H-2d) haplotype, consisted of a synthetic 16-amino-acid oligopeptide derived from sperm whale myoglobin. The immune-enhancing effect was most pronounced for the melittin-derived peptide fragments [Mel(1-16)] and [Mel(17-26)-CONH2]. Antibodies obtained after 3 immunizations with the conjugates recognized the synthetic as well as the native melittin molecule. Our results show that it is possible to markedly enhance a weak hapten-specific immune response by coupling the haptens to a lipopeptide conjugated to a haplotype-specific T helper-cell epitope. The novel conjugates are well suited for the optimization of immunization procedures, and for the development of novel synthetic vaccines.

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Conjugates of Synthetic Lymphocyte-Activating Lipopeptides with Segments from HIV Proteins Induce Protein-Specific Antibody Formation

Cited by 27 publications

References 1 publication

Isolation and characterization of high-mobility-group proteins from maize

Isolation and characterization of high-mobility-group proteins from maize

Identification of a major human immunodeficiency virus‐1 reverse transcriptase epitope recognized by mouse CD4⁺ T lymphocytes

Induction of an epitope-specific humoral immune response by lipopeptide—hapten conjugates: enhancement of the anti-melittin response by a synthetic T helper (T_h)-cell epitope

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Conjugates of Synthetic Lymphocyte-Activating Lipopeptides with Segments from HIV Proteins Induce Protein-Specific Antibody Formation

Cited by 27 publications

References 1 publication

Isolation and characterization of high-mobility-group proteins from maize

Isolation and characterization of high-mobility-group proteins from maize

Identification of a major human immunodeficiency virus‐1 reverse transcriptase epitope recognized by mouse CD4+ T lymphocytes

Induction of an epitope-specific humoral immune response by lipopeptide—hapten conjugates: enhancement of the anti-melittin response by a synthetic T helper (Th)-cell epitope

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Identification of a major human immunodeficiency virus‐1 reverse transcriptase epitope recognized by mouse CD4⁺ T lymphocytes

Induction of an epitope-specific humoral immune response by lipopeptide—hapten conjugates: enhancement of the anti-melittin response by a synthetic T helper (T_h)-cell epitope